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13 Cards in this Set
- Front
- Back
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What are needed for protein synthesis?
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1) Ribosomes
2) Initiation Factors 3) Elongation Factors 4) Release Factors 5) ATP and GTP |
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What is the structure of tRNA?
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"Clover leaf"
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The codon on the mRNA is recognized by the ....... of tRNA.
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Anticodon
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During aminoacylation of a tRNA, what substrates does the synthetase recognize?
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Amino acid and cognate tRNA
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Is the aminoacyl-tRNA bond a high-energy bond?
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Yes
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Why is it said that "Aminoacyl-transfer RNA synthetases read the genetic code"?
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Synthetases ensure the correct match of amino acids to anticodons and then to codons.
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What are the sedimental values of prokaryotic ribosomal subunits?
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50S and 30S
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What are the sedimental values of eukaryotic ribosomal subunits?
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60S and 40S
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What are some inhibitors of eukaryotic translation?
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Cycloheximide and Diptheria toxin
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Which direction (N-->C or C-->N) are peptides synthesized during translation?
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N-->C
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In which direction in mRNA read (5'-->3' or 3'-->5')?
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5'-->3'
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What is the first amino acid? Which tRNA carries it? What is its code?
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Methionine carried by tRNAmet. Coded by AUG.
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Why do the 5' cap and 3' poly(A) tail enhance translation synergistically?
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1) Form closed ring during translation
2) Recruit ribosomes for multiple rounds of translation without having to start from scratch |
