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14 Cards in this Set
- Front
- Back
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Rate of Migration
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Depends on size, shape, and charge of molecule
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Support Medium
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Cullulose acetate or agarose
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Buffer
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Barbital buffer, pH8.6
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Charge
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At pH 8.6, proteins are negatively charged and move toward the anode
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Order of Migration (fastest to slowest)
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Albumin (Largest Fraction), alpha-1 globulin, alpha-2 globulin, beta globulin, gamma globulin
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Urine
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Must be concentrated first because of low protein concentration. Bence Jones proteins migrate to gamma region
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CSF
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Must be concentrated first because of low protein concentration. CSF has a prealbumin band.
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Acute Inflammation
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Increased alpha-1 and alpha-2
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Chronic Infection
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Increased alpha-1, alpha-2, and gamma
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Cirrhosis
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Polyclonal increase in gamma with beta-gamma bridging
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Polyclonal gammopathy
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Diffuse increase in gamma.
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Nephrotic Syndrome
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Decreased albumin. Increased alpha-2.
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Hemolyzed Specimen
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Increased beta or unusual band between alpha-2 beta.
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Plasma
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Extra band between beta and gamma (fibrinogen).
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