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13 Cards in this Set
- Front
- Back
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Molecular genetics |
Studying molecular structure and function of genes and genomes by experimenting in DNA and RNA |
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List the tree steps of brick six acid isolation |
1)Physical or chemical lysis of cells in buffer with detergent. Addition of protease to degrade histones 2) remove proteins by precipitation, organic extraction, binding of brick sic acid to a solid matrix 3) precipitation of nucleic acid with alcohol or elation from solid matrix |
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What is the purpose of restriction enzymes |
Cut up foreign DNA, protect bacteria from viral DNA infection |
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What are the two ways restriction enzymes can act |
As a protein As a homodimer |
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How do bacteria protect their own DNA from restriction enzymes |
Methylation |
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What three things are restriction enzymes used for |
Generate fragments for cloning Determine position of RE sites Confirm identity of DNA molecule |
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How are restriction enzymes named |
Species (Eco = ecoli) Strain (R) Order of discovery (1) |
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How do restriction enzymes differ from each other |
Differ by the site we recognize and the types of ends that result |
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What is the significance of U units to measure restriction enzymes |
1 unit of restriction enzymes digest 1mg of DNA in 1 hour |
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Electrophoresis |
Separation of charged molecules by an electric field |
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What are the limits of agarose and acrylamide |
Agarose for over 100 Acrylamide for under 1000 |
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What must the gel include |
Molecules that bind to DNA |
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Shorter bands travel ... |
Faster |
