A plasmid is circular, relatively small in size, double stranded as a DNA molecule, and is physically distinct from the chromosomal DNA of a cell. Innately found in prokaryotic bacterial cells, plasmids can sometimes be found in eukaryotes as well 1. Plasmids are self-replicating; therefore, when a plasmid is formed, infinite copies of the plasmid can be created if the plasmid is grown in bacteria. 2 Because of the size of plasmids, which can range anywhere from one thousand base pairs up to hundreds of thousands of base pairs, they are relatively easy to work with as modern technology has made the process of both establishing and adjusting plasmids to incorporate the genetic material of interest rather easy. 2One key factor of plasmids is their capability to independently replicate from chromosomal DNA. This replication of plasmids takes place in three
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5For numerous reasons, Type II restriction enzymes are found to be the most valuable in the laboratory. Several of the reasons making these enzymes the most suitable is that no ATP is required when cutting DNA, the cuts made are steady and dependable as they remain on or very close to the recognition sites, and they all merely have restriction activity and no modification activity.4 Within this experiment, three Type II restriction enzymes were used and they include Bgl I, Hind III, and PVU II. Hind III, which is created by Haemophilus influenza, reacts at a temperature of 37°C and cleaves DNA at the particular sequence 5’AAGCTT 3’ when Mg2+ is present.6 PVU II, which is produced from Proteus vulgaris, both recognizes then cuts the DNA sequence, which is double stranded, at 5’ CAGCTG 3’. In addition, PVU cleaves after G-3. Bgl I, which is created from Baccilus globigii, reacts at a temperature of 37°C and cuts the DNA at the specific sequence 5’ AGATCT
5For numerous reasons, Type II restriction enzymes are found to be the most valuable in the laboratory. Several of the reasons making these enzymes the most suitable is that no ATP is required when cutting DNA, the cuts made are steady and dependable as they remain on or very close to the recognition sites, and they all merely have restriction activity and no modification activity.4 Within this experiment, three Type II restriction enzymes were used and they include Bgl I, Hind III, and PVU II. Hind III, which is created by Haemophilus influenza, reacts at a temperature of 37°C and cleaves DNA at the particular sequence 5’AAGCTT 3’ when Mg2+ is present.6 PVU II, which is produced from Proteus vulgaris, both recognizes then cuts the DNA sequence, which is double stranded, at 5’ CAGCTG 3’. In addition, PVU cleaves after G-3. Bgl I, which is created from Baccilus globigii, reacts at a temperature of 37°C and cuts the DNA at the specific sequence 5’ AGATCT