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94 Cards in this Set
- Front
- Back
drops of blood obtained by?
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puncturing/ incision with lancet or other sharp devise
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collection sites for blood droplets?
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fingers(adults) and heels (infants)
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lancet?
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sterile disposable sharp pointed or bladed instrument
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selection of a lancet?
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age of PT
collection site volume required puncture depth needed to collect adequate specimen without injury to bone |
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lasette
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peforates the skin with a laser instead of a sharp instrument
disposable insert prevents cross contamination |
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microcollection devices
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microtubes often refered to as bullets
small plastic tubes used to collect tiny amounts of blood obtained from capillary punctures |
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microcollection devices color coding?
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correspond to ETS blood collection tubes..measured in microliters
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microhematocrit tubes
description |
disposable, narrow bore plastic or plastic clad glass tubes that fill by capillary action
hold 50 to 75 µl of blood |
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microhematocrit tubes used for
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manual hematocrit(Hct)
also called packed cell volume(PVC) |
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microhematocrit tubes color bands
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heparin tubes-red band
nonadditive- blue band |
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microhematocrit tubes ofeten used for age?
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infant and child anemia
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microhematocrit tubes seal with?
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clay or plastic sealants
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CBG (capillary blood gas)
tube description |
long thin narrow-bore capillary tubes..normally plastic
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CBG (capillary blood gas)
common length normal band color? what addative? |
100µl
green heparin |
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CBG (capillary blood gas)
stirrers? |
small metal filings
magnents |
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CBG (capillary blood gas)
end caps? |
plastic to maintain anaerobic conditions
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microscope slides
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blood films for hematology
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terestital fluid
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fluid in tissue spaces between cells
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intracelluar fluid
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fluid within cells
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capillary specimens are a mixture of?
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arterial,venous,capillary blood
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capillary specimens contain a higher proportion of what type blood?
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arterial
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what is falsly elevated if there is tissue fluid contamination or hemolysis
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potassium values
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what are the reasons to choose capillary puncture on adults ot older children?
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no accessible veins
fragile veins veins saved for other procedures thrombotic or clot forming tendencies obtain blood gor POCt(glucose monitoring) |
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why is capillary method preferred for infants/very young children
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small blood volume
(Lg quan removed rapid can cause cardic arrest) difficult other may damage veins injury by restraint preferred specimen for newborn screening |
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what test cannot be performed by skin puncture?
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erythrocyte ded rates
coagulation studies blood cultures any test that requre large vol of blood |
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order of draw for capillary puncture
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1. EDTA
2. other additive 3. Serum |
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capillary puncture steps
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same as others
1. review and accession test required 2. approach, identify, prepare PT 3. verify diet restrictions and latex sensitivity 4. sanitize hands and put on gloves |
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cyanotic
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bluish in color from lack of oxygen
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selection of puncture site for capillary incision
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warm
pink or normal color free of scars, cuts, bruises, rashes NO: cyantotic, edematous, infected |
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recommened site capillary puncture adults and children 1 yr+
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palmar surface of distal or end segment of middle finger or ring finger of nondominat hand
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puncture site capillary puncture adults and children 1 yr+
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central
fleshy portion of finger slightly to side of center perpendicular to grooves in the whorls of fingerprints |
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DO NOT collect capillary puncture from fingers of
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same side as mastectomy
infants and children under 1 yrs side or very tip of finger index finger fifth(little finger) thumb parallel to groves of fingerprint |
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infant capillary puncture recommened site in infants less than 1 yr
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plabntar surface of heel
medial or lateral medial millde great toe to heel lateral-between fouth and fith toe to heel |
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osteomyelitis
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inflammation of bone marrow and adjacent bone
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osteochondtitis
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inflammation of bone and cartilage
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calcaneus
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heel bone
(small or premature infants 2.0 mm below skin surface) |
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posterior curvature infant heel?
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back of heel
puncture may cause bone damage |
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infant capillary puncture precautions
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DO not puncute:
earlobes deeper than 2.0 mm previous puncture sites area between imaginary boundaries posterior curvature of heel area of arch and other areas of foot severly bruised areas(painful, impaired circulation0 |
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Warming area for capillary puncture
Why? |
increases blood flow up to sevenfold(does not significantly alter results)
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specimen from warm site is describes as/
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arterialized (essential when collecting capillary pH or Blood Gases)
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residual alcohol in addition to causing stinging causes?
interfers with? |
causes rapid hemolysis
interferes with glucose testing |
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finger puncture
how to puncture the site? |
flat against skin, in central, fleshy pad, slightly to the side of center and perpendicular to the fingerprint whorls
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heel puncture
how to puncture the site? |
encircle heel, wrap index finger around arch and thumb around bottom, other fingers top of foot
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why wipe away first drop of blood on a capillary collection?
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normally contaminated with excess tissue fluid and may contain alcohol residue (keeps from forming well rounded drop and hemolyze)
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where on the site do you apply pressure to form blood drop for collection?
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gentle intermittent pressure
-tissue surrounding heel -proximal to a finger puncture Do not "milk" site |
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why apply gentle pressure and not "milk" site..
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hemolysis and tissue fluid contamination can result
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CLSI order of draw for capillary specimens
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slides, platelet counts, other hemotology(to aviod the effects of platelet aggregation{clumping} and clotting
next , other anticoagulant containers last serum specimens |
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microhematocrit
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narrow-bore capillary tube
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microhematocrit fill procedures
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hold vertical above drop or horizonal beside drop(will auto fill, may have to have to lower opposite endand bring back into position,while maintain contact with drop)
DO NOT remove from drop or cintinually hold or tip below the site(rusult in air spaces) plug opposite or dry end with clay or sealant when full |
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blood film procedures
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thouch appropiate area of slide to drop
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microcollection or microtube procedures
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hold upright below drop, touch tip of scoop to drop, allow blood to run down inside wall of tube(should only touch blood and not skin..activates platelets/clump/hemolyse)
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microhematocrit can be placed in?? for labeling
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non additive tube or appropate size aliquot tube
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special handling?
ammonia |
crushed ice
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special handling?
cold aggulatinin |
body temp
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special handling?
bilirubin |
light protection
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bleeding persists longer than ??min?
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5 min
notify nurse or phys (notify Pt that bandage kept in place 15 min |
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CBG's
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capillary blood gas specimens
(rarely on adults mostly small children and infants) |
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over production of bilirubin occurs from
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HDN-
accelerated red blood cell hemolysis associated with hemolytic disease of the newborn |
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high levels of bilirubin result in
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jaundice(yellow skin color)
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-impaired bilirubin excretion often results from temporary
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abnormal liver function
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bilirubin can cross the blood-brian barrier in infants causing
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accumulation to toxic levels
brain damage death |
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microhematocrit can be placed in?? for labeling
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non additive tube or appropate size aliquot tube
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special handling?
ammonia |
crushed ice
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special handling?
cold aggulatinin |
body temp
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special handling?
bilirubin |
light protection
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bleeding persists longer than ??min?
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5 min
notify nurse or phys (notify Pt that bandage kept in place 15 min |
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bilirubin breaks down in presence of
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LIGHT
(collect quickly to minimize exposure) (amber tubes) (accurate timing) |
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newborn/neonatal testing is routine and is looking for the presence of?
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genetic disorders(inheritied)
metabolic disorders(chemical changes within liiving cells) hormonal disorders infectious agents (toxoplasma and HIV) phenylketonuria (PKU) galactosemia hypothyroidism (29 specific disorders including hearing loss) functional disorders |
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newborn/neonatal testing is ideally performed when?
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24-72 hrs old
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PKU requires retest. when?
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2 weeks old
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phenylketonuria (PKU)
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genetic disorder-caracterized by a defect in the enzyme that breaks down the amino acid phenylalanine..converts to amino acid tyrosine
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galactosemia
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inherited disorder characterized by lack of enzyme needed to cover milk sugar galactose in to glucose
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hypothyroidism
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disorder characterized by insufficent levels of thyroid hormaines
can be inherited or noninherited |
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phenylketonuria (PKU)
treatment? untreated? |
No but can be treated..diel low in phenylalanine
untreated can lead to toxic levels..brian damage..mental retardation |
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hypothyroidism
treatment? untreated? |
treated bu supplying TSH hormone orally
untreated it hinders growth and brain development |
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galactosemia
treatment? untreated? |
remove all milk and dariy products from diet
untreated..within a weekk, fail to thrive (anorexia, diarrrhea,vomiting..may starve to death..fail to grow..mentally handicapped..cataracts |
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blood spot collection
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absorption onto circles printed on special type of filter paper (blood filled circles are refered to "blood spots")
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blood spot collection procedure
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fill from one side only..use one drop to fill circle..allow to air dry in elevated horizonal position..no heat or sunlight..touch with gloves only..do not stack can cross contaminate..place in special envelope when dry
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blood film or smear
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drop of blood spread thin on a microscope slide (stained)
usually made from EDTA tube in the lab |
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blood film or smear used for?
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manual differential(diff)
(number,type,characteristics of blood cells are determined |
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leukocyte alkaline phosphate (LAP) stain ore score
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blood smear from a fresh frop of blood from a fingertip (nto in contact with EDTA) collect forts to avoid platelt clumping
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what is a acceptable blood film or smear
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1/2 to 3/4 of surface of slide
no holes,lines,or jagged edges |
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blood film or smear "feather" refer to?
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thinnest area of a properly made smear, one cell thick,where the differential preformed
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thick blood smear used for
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detect presence of malaria
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malaria
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disorder caused by four species of parasitic sporozoan (types of portozoa) organisms called plasmodia
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malaria transmitted by
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infected female anopheles mosquitoes
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malaria symptoms
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fever, chills at regular intervals
..progressive destructtion of RBCs in certain types causes severe anemia.. |
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malaria smears collected?
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stat or timed intervals
-most commonly just prior to onset of fever and chills |
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thick smear preparation
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very large drop of blood placed in center of glass slide and spread with corner of another slide or cover slip until size of dime..dry 2hrs then fresh diluted giemsa stained
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giemsa stain
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water based stain that lysis the RBCs and makes organism easier to see
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what test requires arterialized specimen
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CBGs
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concentration of this substance is higher in capillary blood than in venous blood
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glucose
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heel puncture lancet should not be deeper than
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2.0mm
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when making a routime blood smear the pusher slide is normally held at an angle of
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30degrees
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