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94 Cards in this Set

  • Front
  • Back
drops of blood obtained by?
puncturing/ incision with lancet or other sharp devise
collection sites for blood droplets?
fingers(adults) and heels (infants)
lancet?
sterile disposable sharp pointed or bladed instrument
selection of a lancet?
age of PT
collection site
volume required
puncture depth needed to collect adequate specimen without injury to bone
lasette
peforates the skin with a laser instead of a sharp instrument
disposable insert prevents cross contamination
microcollection devices
microtubes often refered to as bullets
small plastic tubes used to collect tiny amounts of blood obtained from capillary punctures
microcollection devices color coding?
correspond to ETS blood collection tubes..measured in microliters
microhematocrit tubes
description
disposable, narrow bore plastic or plastic clad glass tubes that fill by capillary action
hold 50 to 75 µl of blood
microhematocrit tubes used for
manual hematocrit(Hct)
also called packed cell volume(PVC)
microhematocrit tubes color bands
heparin tubes-red band
nonadditive- blue band
microhematocrit tubes ofeten used for age?
infant and child anemia
microhematocrit tubes seal with?
clay or plastic sealants
CBG (capillary blood gas)
tube description
long thin narrow-bore capillary tubes..normally plastic
CBG (capillary blood gas)
common length
normal band color?
what addative?
100µl
green
heparin
CBG (capillary blood gas)
stirrers?
small metal filings
magnents
CBG (capillary blood gas)
end caps?
plastic to maintain anaerobic conditions
microscope slides
blood films for hematology
terestital fluid
fluid in tissue spaces between cells
intracelluar fluid
fluid within cells
capillary specimens are a mixture of?
arterial,venous,capillary blood
capillary specimens contain a higher proportion of what type blood?
arterial
what is falsly elevated if there is tissue fluid contamination or hemolysis
potassium values
what are the reasons to choose capillary puncture on adults ot older children?
no accessible veins
fragile veins
veins saved for other procedures
thrombotic or clot forming tendencies
obtain blood gor POCt(glucose monitoring)
why is capillary method preferred for infants/very young children
small blood volume
(Lg quan removed rapid can cause cardic arrest)
difficult
other may damage veins
injury by restraint
preferred specimen for newborn screening
what test cannot be performed by skin puncture?
erythrocyte ded rates
coagulation studies
blood cultures
any test that requre large vol of blood
order of draw for capillary puncture
1. EDTA
2. other additive
3. Serum
capillary puncture steps
same as others
1. review and accession test required
2. approach, identify, prepare PT
3. verify diet restrictions and latex sensitivity
4. sanitize hands and put on gloves
cyanotic
bluish in color from lack of oxygen
selection of puncture site for capillary incision
warm
pink or normal color
free of scars, cuts, bruises, rashes
NO: cyantotic, edematous, infected
recommened site capillary puncture adults and children 1 yr+
palmar surface of distal or end segment of middle finger or ring finger of nondominat hand
puncture site capillary puncture adults and children 1 yr+
central
fleshy portion of finger
slightly to side of center
perpendicular to grooves in the whorls of fingerprints
DO NOT collect capillary puncture from fingers of
same side as mastectomy
infants and children under 1 yrs
side or very tip of finger
index finger
fifth(little finger)
thumb
parallel to groves of fingerprint
infant capillary puncture recommened site in infants less than 1 yr
plabntar surface of heel
medial or lateral
medial millde great toe to heel
lateral-between fouth and fith toe to heel
osteomyelitis
inflammation of bone marrow and adjacent bone
osteochondtitis
inflammation of bone and cartilage
calcaneus
heel bone
(small or premature infants 2.0 mm below skin surface)
posterior curvature infant heel?
back of heel
puncture may cause bone damage
infant capillary puncture precautions
DO not puncute:
earlobes
deeper than 2.0 mm
previous puncture sites
area between imaginary boundaries
posterior curvature of heel
area of arch and other areas of foot
severly bruised areas(painful, impaired circulation0
Warming area for capillary puncture
Why?
increases blood flow up to sevenfold(does not significantly alter results)
specimen from warm site is describes as/
arterialized (essential when collecting capillary pH or Blood Gases)
residual alcohol in addition to causing stinging causes?
interfers with?
causes rapid hemolysis
interferes with glucose testing
finger puncture
how to puncture the site?
flat against skin, in central, fleshy pad, slightly to the side of center and perpendicular to the fingerprint whorls
heel puncture
how to puncture the site?
encircle heel, wrap index finger around arch and thumb around bottom, other fingers top of foot
why wipe away first drop of blood on a capillary collection?
normally contaminated with excess tissue fluid and may contain alcohol residue (keeps from forming well rounded drop and hemolyze)
where on the site do you apply pressure to form blood drop for collection?
gentle intermittent pressure
-tissue surrounding heel
-proximal to a finger puncture
Do not "milk" site
why apply gentle pressure and not "milk" site..
hemolysis and tissue fluid contamination can result
CLSI order of draw for capillary specimens
slides, platelet counts, other hemotology(to aviod the effects of platelet aggregation{clumping} and clotting
next , other anticoagulant containers
last serum specimens
microhematocrit
narrow-bore capillary tube
microhematocrit fill procedures
hold vertical above drop or horizonal beside drop(will auto fill, may have to have to lower opposite endand bring back into position,while maintain contact with drop)
DO NOT remove from drop or cintinually hold or tip below the site(rusult in air spaces)
plug opposite or dry end with clay or sealant when full
blood film procedures
thouch appropiate area of slide to drop
microcollection or microtube procedures
hold upright below drop, touch tip of scoop to drop, allow blood to run down inside wall of tube(should only touch blood and not skin..activates platelets/clump/hemolyse)
microhematocrit can be placed in?? for labeling
non additive tube or appropate size aliquot tube
special handling?
ammonia
crushed ice
special handling?
cold aggulatinin
body temp
special handling?
bilirubin
light protection
bleeding persists longer than ??min?
5 min
notify nurse or phys
(notify Pt that bandage kept in place 15 min
CBG's
capillary blood gas specimens
(rarely on adults mostly small children and infants)
over production of bilirubin occurs from
HDN-
accelerated red blood cell hemolysis associated with hemolytic disease of the newborn
high levels of bilirubin result in
jaundice(yellow skin color)
-impaired bilirubin excretion often results from temporary
abnormal liver function
bilirubin can cross the blood-brian barrier in infants causing
accumulation to toxic levels
brain damage
death
microhematocrit can be placed in?? for labeling
non additive tube or appropate size aliquot tube
special handling?
ammonia
crushed ice
special handling?
cold aggulatinin
body temp
special handling?
bilirubin
light protection
bleeding persists longer than ??min?
5 min
notify nurse or phys
(notify Pt that bandage kept in place 15 min
bilirubin breaks down in presence of
LIGHT
(collect quickly to minimize exposure)
(amber tubes)
(accurate timing)
newborn/neonatal testing is routine and is looking for the presence of?
genetic disorders(inheritied)
metabolic disorders(chemical changes within liiving cells)
hormonal disorders
infectious agents (toxoplasma and HIV)
phenylketonuria (PKU)
galactosemia
hypothyroidism
(29 specific disorders including hearing loss)
functional disorders
newborn/neonatal testing is ideally performed when?
24-72 hrs old
PKU requires retest. when?
2 weeks old
phenylketonuria (PKU)
genetic disorder-caracterized by a defect in the enzyme that breaks down the amino acid phenylalanine..converts to amino acid tyrosine
galactosemia
inherited disorder characterized by lack of enzyme needed to cover milk sugar galactose in to glucose
hypothyroidism
disorder characterized by insufficent levels of thyroid hormaines
can be inherited or noninherited
phenylketonuria (PKU)
treatment?
untreated?
No but can be treated..diel low in phenylalanine
untreated can lead to toxic levels..brian damage..mental retardation
hypothyroidism
treatment?
untreated?
treated bu supplying TSH hormone orally
untreated it hinders growth and brain development
galactosemia
treatment?
untreated?
remove all milk and dariy products from diet
untreated..within a weekk, fail to thrive (anorexia, diarrrhea,vomiting..may starve to death..fail to grow..mentally handicapped..cataracts
blood spot collection
absorption onto circles printed on special type of filter paper (blood filled circles are refered to "blood spots")
blood spot collection procedure
fill from one side only..use one drop to fill circle..allow to air dry in elevated horizonal position..no heat or sunlight..touch with gloves only..do not stack can cross contaminate..place in special envelope when dry
blood film or smear
drop of blood spread thin on a microscope slide (stained)
usually made from EDTA tube in the lab
blood film or smear used for?
manual differential(diff)
(number,type,characteristics of blood cells are determined
leukocyte alkaline phosphate (LAP) stain ore score
blood smear from a fresh frop of blood from a fingertip (nto in contact with EDTA) collect forts to avoid platelt clumping
what is a acceptable blood film or smear
1/2 to 3/4 of surface of slide
no holes,lines,or jagged edges
blood film or smear "feather" refer to?
thinnest area of a properly made smear, one cell thick,where the differential preformed
thick blood smear used for
detect presence of malaria
malaria
disorder caused by four species of parasitic sporozoan (types of portozoa) organisms called plasmodia
malaria transmitted by
infected female anopheles mosquitoes
malaria symptoms
fever, chills at regular intervals
..progressive destructtion of RBCs in certain types causes severe anemia..
malaria smears collected?
stat or timed intervals
-most commonly just prior to onset of fever and chills
thick smear preparation
very large drop of blood placed in center of glass slide and spread with corner of another slide or cover slip until size of dime..dry 2hrs then fresh diluted giemsa stained
giemsa stain
water based stain that lysis the RBCs and makes organism easier to see
what test requires arterialized specimen
CBGs
concentration of this substance is higher in capillary blood than in venous blood
glucose
heel puncture lancet should not be deeper than
2.0mm
when making a routime blood smear the pusher slide is normally held at an angle of
30degrees