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268 Cards in this Set

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Definitive host

Defined as the host that harbours the Adult parasite or the Sexual stage

Intermediate Host

Defined as the host that harbours the larval stage or the Asexual stage

Nematodes - Roundworms


Trematodes - Flukes


Cestodes - Tapeworms

Other common names for the helminthes?

E. histolytica

What is the only pathogenic amoeba?

Balantidium coli

The only important parasite under ciliates?

A.lumbricoides

Giant intestinal roundworm/eelworm?

A.lumbricoides

Largest nematode?

S.stercoralis

Smallest nematode?

D.latum

Longest tapeworm?

E.granulosus

Shortest tapeworm according to length?

Trichuris trichiura

Whipworm?

E.vermicularis

Pinworm/Seatworm?

Capillara philippinensis

Pudoc worm?

Trichina worm or pork muscle round worm

T.spiralis is also known as?

Strongyloides stercoralis

Threadworm?

N.americanus

New world hookworm?

A.duodenale

Old world hookworm?

A.caninum

Dog hookworm?

A.braziliense

Cat hookworm?

T.canis

Ascaris in dogs?

T.cati

Ascaris in cats?

W.bancrofti

Bancroft's filaria?

B.malayi

Malayan filaria?

Eyeworm

Loaloa? other name?

O.volvulus

Convuluted filarial?

M.ozzardi

Ozzard's filarial?

M.perstans

Persistent filarial?

Dragon worm, Guinea worm, serpent worm

other name for D.medinensis?

Rat lung worm

common name for A.cantonensis

Dog heart worm

other name for D.immitis?

S.japonicum

Oriental blood fluke?

S.mansoni

Manson's Blood fluke

Vesical Blood fluke

Other name for S.haematobium?

P.westermani

Oriental lung fluke?

Sheep Liver fluke

Other name for F.hepatica?

Chinese liver fluke/ Oriental liver fluke

Other name for C.sinensis?

O.felineus

Cat liver fluke?

Garrison's fluke

other name for E.illocanum?

Busk Fluke


Giant Intestinal Fluke

other name for F.buski?

Von siebold fluke


Dwarf fluke

Other name for H.heterophyes?

Yokogawai fluke

Common name for M.yokogawai?

Fish broad tapeworm


Russian broad tapeworm

Common name of D. latum?

Pork Tapeworm or Armed tapeworm

common name of T.solium?

Beef tapeworm or unarmed tapeworm

common name of T.saginata?

H.diminuta

Rat tapeworm?

H.nana

Dwarf tapeworm?

Dog tapeworm or Double pired tapeworm

Common name of D.caninum?

Hydatid worm

Common name of E.granulosus?

-Non Motile


-Usually the infective form


-Found in formed feces


-Smaller than trophozoite


-Multi-nucleated


-Resistant to damage


-Use Iodine for better visualization

Describe cyst

If the protozoa has cyst then it is the infective form if it only has trophozoite then the infective form is trophozoite

Rule for protozoans in terms of infectivity

Metacyst

Transitory stage prior to excystation

Precyst

Transitory stage prior to encystation?

Explain transitory stages for amoebas?

Naegleria


Acantamoeba

2 Exampkes of facultative amoebas (could live outside body)

Entamoeba gingivalis



Habitat: Buccal Cavity

All amoebas lives in the colon except_______ w/c lives in ______

Entamoeba histolytica

The only pathogenic amobe?

-Entamoeba gingivalis -Dientamoeba fragilis



They only have trophozoite forms

All amoebas have cyst and troph forms except?________

-E. gingivalis & D.fragilis



Since they only have the trophozoite form, it is their sole infective stage

All amoebas have cyst as infective form except?

E. gingivalis



Since its the only one w/ mouth as its habitat it is transmitted through Oral Contact

All Amoebas have Fecal-Oral Route as MOT except?

Dientamoeba fragilis



Stained by trichrome stain

No longer an amoeba but now classified as a flagellate? Eggs of E.vermicularis might carry this causing double infection? Also could be stained by what?

-Motile


-Usually the Non-infective form


-Found in Liquid or Watery stool


-Larger than cyst


-Usually uni-nucleated


-Susceptible to damage


-Not suited for: Concentration Techniques

Describe a trophozoite

Permanent stain



E.g: Gomori's Trichrome, Iron Hematoxylin

Trophozoites are better visualized using? Examples of which?

FALSE



Iodine makes trophozoites disappear

Iodine could not cause disappearance of Trophozoite true or false?

4



E.histolytica has 1-4 nuclei but only those w/ 4 nuclei are considered infective

At what number of nuclei of E.histolytica is it considered infective?

Entamoeba nana

Smallest intestinal protozoa?

E.nana

Amoeba with 1-8 nuclei?

E.nana

Amoeba with 4 nuclei but with Karyosome on the end causing it to be called "CROSS-EYED CYST"

Iodamoeba butschlii

Amoeba with only 1 nuclei?

Stained by IRON HEMATOXYLIN



Color: Black

Chromatoidal bodies of protozoan cysts are stained with _____ causing ______ color

E.histolytica

Cigar shaped or Sausage shaped chromatoidal bodies?

Entamoeba coli

Broomstick or Splintered glass like chromatoidal bodies in cyst?

E.nana

Small and spherical chromatoidal bodies in cysts belong to?

Iodamoeba butschlii

No chromatoidal bodies but has a big glycogen mass instead that could be stained w/ iodine causing this to be called "IODINE CYST"

Pseudopodia

Organ of trophozoites that makes movement possible?

-E.histolytica - long & finger like pseudopia



-D.fragilis - hyaline, multiple, leaf like pseudopodia

All trophozoite have blunt & rounded pseudopodia except?

E. histolytica



D.fragilis

Progressive & Directed motility of trophozoite?

E.coli

Sluggish & Non-directed movement of trophozoite

E.nana & I.butschlii

Sluggish but progressive movement of trophozoite

E.gingivalis



It has multiple pseudopodia that is why it is quite active

Moderately active movement of trophozoite

E.histolytica

Bull's eye karyosome is found in what trophozoite?

D.fragilis

Tetrakaryosome trophozoite is found on what?

E.histolytica

Amoeba with ingested rbcs on their cytoplasm?

E.gingivalis

Amoeba with ingested WBCs and food vacuoles in their cytoplasm?

E.dispar


-Currently closest morphology w/E. histolytica


-To differentiate do PCR or Immunoassay



E.hartmanni


-"SMALL RACE HISTOLYTICA"


-Previously closest to E.histolytica but does not ingest RBC



E.polecki


-Parasitize monkeys & pigs


-Has only one nuclei

3 amoebas that closely resembles E.histolytica?

Entamoeba nana (smallest)



Balantidium coli (largest)

Smallest intestinal protozoa?



Largest intestinal protozoa?

Balantidium coli

Directional & tumbling motility of trophozoite?

Balantidium coli

Causes intraintestinal lesions?

Large intestine

Habitat of B.coli?

Macro-nucleus : for vegetation



Micro-nucleus: for reproduction

Balantidium coli cyst has distinguishing feature that is only unique to it. What is it?

Stool exam



Entero test


-


2 Tests used to diagnose G.lamblia

Trichomonas specie

What are the only flagellates that consist of only the trophozoite form?

Trichomonas tenax


Trichomonas Vaginalis

What are the species considered as atrial flagellates?

G.lamblia


C.mesnilii


T.hominis

Luminal flagellates?

Giardia lamblia

The only small intestine inhabiting protozoa?

Trichomonas tenax



-Also called "Flagellate of the mouth"

All flagellates are transmitted through fecal-oral route except?

Steatoherric / Contains fats



-Because of sucking discs of G.lamblia that causes malabsorption

Distinguishing feature of stool infected with G.lamblia?

Giardia lamblia

Bilaterally symmetrical, "KITE-LIKE MOTILITY" "FALLING-LEAF LIKE MOTILITY" "OLD MAN WITH GLASSES" Is used to describe what?

Gardia lamblia

Causes GAY-BOWEL DISEASE?

Chilomastix mesnilii

cyst is commonly referred to as "NIPPLE-LEMON LIKE"

Chilomastix mesnilii

Trophozoite showing a "SHEPHERD-CROOK'S APPEARANCE" "CORK-SCREW" "SPIRAL-JERKY"

Trichomonas vaginalis

Only pathogenic trichomonas

LONGEST - T.hominis


SHORTEST - T.vaginalis


AS LONG AS COSTA - T.tenax

Umdulating membrane of trichomonas species?

T.vaginalis

"FAST-JERKY MOVEMENT"

Urine


Vaginal discharge

Specimen for diagnosis of T.vaginalis?

Enteromonas hominis


-Binucleate, Rapid-Jerky Movement



Retortomonas intestinalis


-"BIRD'S BEAK FIBRILLAR APPEARANCE"

2 rare intestinal flagellates and their distinguishing characteristic?

L.donovani- mucosal organs


L.tropica- Lymphoid tissue


L.braziliense- Nose, mouth pharynx

Location of Leishmania species?

Amastigote -Diagnostic and seen in man intracellularly



Promastigote- Develop in vectors

2 Developmental stages of Leishmania species?

Sandflies (Phlebotomus papatazi)

Vector for Leishmania?

Skin Inoculation

MODE OF TRANSMISSION FOR LEISHMANIA?

Leishmania donovani

What causes Kala-azar fever?/Dum-Dum fever/Black disease/ Visceral Leishmaniasis

Leishmania donovani

Causes worst Leishmaniasis?

Leishmania tropica

Causes cutaneous leishmaniasis/oriental sore/Aleppo/Baghdad boil/Delhi ulcer

Leishmania braziliense

Causes muco-cutaneous leishmaniasis/espundia/Chiclero ulcer/UTA

Leishmania braziliense

Causes leprosy like lesions, "TAPIR NOSE"

EXTRACELLULAR


-Trypanosoma gambiense


-Trypanosoma rhodesiense



INTRACELLULAR


-Trypanosoma cruzi

Species of trypanosomes

Tse-tse fly

Vector of EXTRACELLULAR Trypanosomes? (T.rhodisiense, T.gambiense)

Epimastigote -> Metacyclic Trypanosome -> Trypomastigote



Metacylcic Trypanosome = Infective to man



Trypomastigote = Diagnostic & develop in man

Life cycle of trypanosomes

Trypanosome chancre -Develops in bite site


Winterbottom sign - enlargement lymphnodes


Kerandell sign- All signs related to CNS

African Sleeping sickness?

Assasin, Cone-Nose, Kissing, Triatoma, Redavid, Panstrongylus,Rhonius Bug

Vector of INTRACELLULAR Trypanosomes? (T.cruzi)

T.gambiense


T.rhodisiense

What are considered mmembers of the Brucei complex?

West African Sleeping Disease / Old world trypanosomiasis/ Gambian Trypanosomiasis

T.gambiense causes what disease?

East african trypanosomiasis/ Rhodesian trypanosomiasis

T.rhodesiense causes what disease?

Chaga's disease/ South American trypanosomiasis/ New World trypanosomiasis

Trypanosoma cruzi causes what disease?

Diagnostic stage: Trypomastigote & Amastigote



Infective stage: Metacyclic Trypanosome

Diagnostic stage in T.cruzi and Infective stage

Chagoma (Lesions at bite size)


Romana's Sign (Swelling of eyelids)

Symptoms of T.cruzi?

C, S, U shape

Trypomastigote of T.cruzi assumes what shape?

Female anopheles species

Vector for the plasmodium species?

Plasmodium knowlesi

Pllasmodium specie that infects old world monkeys/ machques

Plasmodium falciparum

Causes the most severe form of malaria?

Plasmodium falciparum

Infects all ages of RBCs

Plasmodium ovale , Plasmodium vivax

Infects young, juvenile forms of RBCs?

Plasmodium malaria

Affects mature RBCs?

P.falciparum - No effect


P.malariae - Reduced RBCs


P.vivax - Enlarge smooth RBCs


P.ovale- Enlarge fimbriated RBCs

Effects to RBCs of Plasmodium species?

Plasmodium falciparum - maurer's dots



Plasmodium malaria- Ziemann's dots



Plasmodium vivax - Schauffner's dots



Plasmodium ovale- James dots or Schauffner's (If no james is available)

Cytoplasmic inclusions or malarial stipplings of Plasmodium?

T.rhodisiense



-Patient dies before CNS signs starts appearing

Trypanosome causing rapid and fast death?

SCHIZOGONY (Asexual stage) - Happens in Man



SPOROGONY (Sexual Stage) - Happens in Vectors

2 life cycles of plasmodium species and their location

P.falciparum - 36 Hours


P.malaria - 72 Hours


P.ovale & P.vivax - 48 Hours

Time of paroxysm or the moment where RBCs burst? For the plasmodium species?

Sporozoites

infective to man form of the plasmodium?

EXO-ERYTHTOCYTIC CYLCE


Sporozoites -> Liver -> Cryptozoic Schizonts -> Merozoites -> Infected cell ruptures merozoites liberated



ERYTHROCYTIC CYCLE


Merozoites infect RBC -> Ring form trophozoites -> Schizont -> Merozoite -> infected cell bursts liberating merozoites



Merozoites not becoming schizonts will eventually become Gametocytes which will infect female anopheles

2 cycle of a Plasmodium specie

Gametocytes

Infective form of Plasmodium to vector

Gametocytes -> Mature gametes -> Zygote egg -> Ookinete -> Oocyst -> sporozoites within sporocyst -> rupture of sporocyst liberating sporozoites -> migration of sporozoites on the salivary glands of anopheles

explain the sporogony cycle in female anopheles

Thick smear


Thin smear

Gold standard in detection of malaria?

Plasmodium vivax


Plasmodium ovale



-This two species causes reoccurring malaria because of hidden hypnozoites in liver cells

Hypnozoites are part of tge lifecycle of what plasmodium species?

P.falciparum - Malignant tertian malaria


P.vivax- Benign tertian malaria


P.ovale- Ovale malaria


P.malaria - Quartan malaria

Plasmodium species and the malaria they cause?

P.falciparum



-Blackwater syndrome is where there is massive hemoglobinuria

Black water fever is also caused by?

P.vivax

Duffy negative individuals shows resistance to?

P.falciparum

people having Sickle cell trait anemia are resistant to?

Relapse of fever due to P.falciparum being increase in too many stages of rbcs

What is Recrudesence?

Before height of fever

When is the best time to collect blood for the diagnosis of malaria?

Thin smear is for identification


Thick smear is for rapid diagnosis

Thin smear is for ____ while thick smear is for ____

Giemsa:


(Thick) - Dehemohlobinize by Distilled H2O



(Thin) - Fixed by Alcohol

In giemsa method thick smear is DEHEMOGLOBINIZE by ______ while thin smear is fixed by ____


Fixation

In wright's stain _____ is no longer needed

2% Formalin with 1% Acetic acid

In Delafield Hematoxylin to dehemoglobinize use?

Ringed form & Gametocytes

What stages are seen in blood in P.falciparum infection?

Plasmodium malariae

Rosette/ daisy formation of merozoites are found in?

Plasmodium falciparum

Aplique/ Accole/ Headset form of ring form trophozoite is found where?

Plasmodium malariae

Band form trophozoite is found where?

Plasmodium falciparum

Crescent form/Sausage form/ Banana shaped gametocytes is found where?

Babesia microti

same cycle as plasmodium specie but no hepatic cycle?

Babesia microti

Maltese cross merozoite?

It is transmitted through tick bites


by Tick genus: Exodes

How is B.microti transmitted?


Vector of Babesia microti?

Cyst: "OUTER WRINKLED WALL"


Culture: Non nutrient agar with E.coli


Disease: Causes infection among soft contact lense wearers

Characteristic of Acantamoeba ?

Naegleria

An amoebo-flagellate that has two trophozoite form the 1.) Amoeboid form 2.) The Flagellated form

T.gondii



DH: Cats


IH:Man


Infective stage: Oocyst


Mode of transmission: Ingestion of oocyst from cat litter



Other MOT: Inhalation, Transplacental, Organ transplant,Ingestion of oocyst from infected animals

Toxoplasma gondii hosts? DH?IH? Infective stage?Most common mode of MOT?

Sabin Feldman Dye test


-Sero test


- (+) result : Lost affinity for methylene blue

Lab diagnosis of T.gondii?Name of test?

Cryptosporidium parvum

What is diagnosed through DFS, Conc. technique like Sheeters Concentration , modified AF staining?

Vacuolated

Most common form of Blastocystis Hominis?

Male : Curved Tail


Female : Straight tail

In Males & Females of nematodes what are the distinguishing characteristics?

Cytosome -mouth


Cytopyge- Anus

What do you call the mouth and anus of B.coli?

Cytosome -mouth


Cytopyge- Anus

What do you call the mouth and anus of B.coli?

Cephalic receptor - Amphids


Caudal receptor - Phasmids

Cephalic (Head) chemoreceptor is called?


Caudal (Tail) Chemoreceptor is called?

All have Amphids (Head receptor) but not all have Phasmids (Tail receptors)

In nematodes all have _____ but not all have _____

PHASMIDS (S.H.A.E)


S - S.stercoralis


H - All Hookworms


A - Ascaris lumbricoides


E - Enterobius vermicularis



APHASMIDS (T.T.C)


T - Trichuris trichiura


T - Trichinella spiralis


C - Capillara philippinensis

What are examples of Phasmids and Aphasmids?

SMALL INTESTINE (C.A.S.H)


C - C.philippinensis


A - A.lumbricoides


S - S.stercoralis


H - Necator & A.duodonale



LARGE INTESTINE (T.E)


T - Trichuris trichiura


E - Enterobius vermicularis



EXTRAINTESTINAL


-All Filariae


-Trichinella spiralis is both intestinal & extraintestinal

Location of Nematodes summarize?

OVIPAROUS(H.A.T)- eggs w/ no larvae


H - Hookworms


A - A.lumbricoides


T - Trichuris trichiura



OVOVIPAROUS/OVIVIPAROUS (S.E) - Eggs w/Larva


.vermicularis



VIVIPAROUS/LARVIPAROUS -no egg just larva


S - S.stercoralis E- E.vermicularisVIVIPAROUS/LARVIPAROUS -no egg just larva -Trichinella spiralis-All filarial worms-D.medinensis


-Trichinella spiralis


-All filarial worms


-D.medinensis


Classification of female nematodes & their samples?

Embryonated egg

Infective stage of A.lumbricoides, Hookworms, E.vermicularis?

Egg -> Larva -> Man & woman -> Egg

life cycle of Nematodes?

A.lumbricoides

Only nematode with trilobate lips?

Fertilized (Corticated) : ovoid, thick shell, has mamilliary coat outside


*Only fertilized eggs continue on the life cycle



Unfertilized (Uncorticated) : Irregular ovoid, thin shell , unorganize germ cell



240,000 Eggs / day

Eggs of ascaris lumbricoides? differentiate? How many?

Male: Dies after mating


Females: At night lays all her eggs causing anus pruritus then dies



4,000-6,000 EGGS / Day

Distinguishing characteristics of male and female E.vermicularis?

A.lumbricoides


T.trichiura


E.vermicularis


T.canis


T.cati

Acquired through ingestion of embryonated egg?

T.spiralis

Acquired through ingestion of encysted larva?

C.philippinensis


D.medinensis


A.cantonensis

Acquired thru ingestion of ingested larva

S.stercoralis


Hookworms

Acquired through filariform larval skin penetration?

W.bancrofti


B.malayi


L.loa


O.volvulus


M.ozzardi


M.perstans

Acquired thru inoculation by vectors?

Undergoes : Embryonation


No : No lung migration

T.trichiura infection undergoes?_____ but no _____

Autoinfection: Hand to Mouth contamination


Retroinfection: Re-entry if parasites through anus


Pruritus ani: When itching at your anus during scratching



Are all present in? E.vermicularis

Autoinfection:


Retroinfection:


Pruritus ani:



Are all present in?

Tichuris trichiura

Has Japanese lantern ova?

Trichuris trichiura

Most difficult to expel using anti-helminthic drug?

Larva: Migrates to lungs


Embryonation: (+)


Dse cause: Ascariasis & Pneumonitis


Spx: Stool (Fertilized&Unfertilized) & Sputum (Larva)

Life cycle of A.lumbricoides?

No

does E.vermicularis have embryonation & lung migration?

Diagnostic: Eggs in stool


Has lung migration

C.philippinensis?


Dx:


Is lung migration present?

Borborygmi = Gurgling of the stomach

Capillaria philippinensis causes capillariasis a mystery diseasr in which the characteristic symptoms is?

TYPICAL


-Peanut - shaped


-Flattened polar plugs


-Striated shell


-With single non-segmented cell



ATYPICAL


-not peanut shaped


-no flattened polar plugs


-contains multi-segmented cell or even larva


-Shell not striated

2 Types of OVA of C.philippinensis?

Typical female: Female whose uterus contains 1 row of eggs



Atypical female: Female with uterus containing 2-3 rows of eggs

2 types of females of C.philippinensis?

Egg -> Rhabditiform -> Filariform -> Adult

Life cycle of strongyloides & hookworm?

RHABDITIFORM


-Is considered as Feeding stage


-It has open mouth


-Shorter & robust


-Not infective


-Considered as diagnostic in S.stercoralis



FILARIFORM


-Nonfeeding form


-Closed mouth


-Longer & slender than rhabditiform


-Usually infective


-Could penetrate skin

Differentiate Rhabditiform & Filariform?

D.immitis

"COIN LESIONS" is characteristic of what?

A.cantonensis

"BARBED POLE APPEARANCE" characteristic of?

A.castillani

Amoebic KERATITIS Is caused by what?

Unsheated; nuclei extend to tip of hooked tail

What is the characteristic tail morphology of Mansonella streptocerca?

Leishmania spp: Sandfly (Phlebotomus)


T.brucei complex: TseTse Fly


T.cruzi: Bug


Plasmodium spp: Female Anopheles


B.microti: Exodes (tick)


W.bancrofti: Aedes, Culex, Anopheles (A.C.A)


B.malayi: Mansonia (Fresh water plant)


Loaloa: Mango/Deer/Chrysops Fly


O.volvulus: Black/Simullium Fly


M.ozzardi: Cullicoides Fly


M.perstans: Cullicoides Fly

Provide the vectors of each


Leishmania spp:


T.brucei complex:


T.cruzi:


Plasmodium spp:


B.microti:


W.bancrofti:


B.malayi


Loaloa


O.volvulus:


M.ozzardi:


M.perstans:

Of Skin penetrstion of Hookworms

Koolie itch / Dew itch happens because?

N.americanus - 10,000 eggs/day


A.duodenale - 20,000 eggs / day

Number of eggs for each?


N.americanus?


A.duodenale?

Direct cycle: (Parasitic Cycle)


-Pathenogenic female


-no male


-Lung migration (+)


-No embryonation


-Eggs hatch in small intestine


Indirect Cycle:


-if soil is moist Rhabditiform will grow to Adult already


Autoinfection:


-Rhabditiform becomes filariform in small intestine


Life cycles of Srrongyloides stercoralis?

Eggs -> Rhabditiform -> Goes to soil -> Feeds -> Shreds -> Filariform -> Lungs -> S.I -> Adult



(+)for lung migration & embryonation

Lifecycle hookworms

Differentiate the hookworm species according to teeth

Differentiate Necator americanus from A.duodenale according to dorsal rays & copulatory spicules

Differentiate rhabditiform of hookworm and strongyloides stercoralis

Differentiate Filariform of S.stercoralis & hookworm

Ascaris, Hookworm, Trichuris

Unholy three?

Ascaris, strongyloides, hookworm

What parasite does lung migration?

Adult parasite : S.I


Larva: Found in Striated muscle

2 locations of Trichinella spiralis?

Infective form: Larva


Female: Larviparous


MOT: Ingestion of larva from uncooked pork


Gravid female lays:1500 larvas / day


d female lays:1500 larvas / day



Infective stage of trichinella? what type o female? MOT?How many larvas delivered per day?

4 weeks

How many weeks does the eaten larva of T.spiralis mature in the small intestine?

Trichenella spiralis

What parasite infectipn wherein man is considered as tje Terminal Host?'

Periorbital edema

What do you call the encysment of larva of trichina worm in the eyes?

Muscle Biopsy- Most accurate


Bentonite flocculation test- BACHMAN

2 ways to diagnose trichinosis?

Adult -> Rhabditiform/L3 -> Microfilaria -> Adult



L3- Infective form


Microfilaria- Diagnostic to man


Lifecycle of Filariforms? Infective form? Diagnostic form?

W.bancrofti: Lower lymphatics


B.malayi: Upper lymphatics


Loaloa: Subcutaneous tse


O.volvulus: Subcutaneous tse


M.ozzardi: Body cavities


M.perstans:Body cavities

Summarize the areas that the filarial worms affects

W.bancrofti-Aedes, Culex, Anopheles


B.malayi- Mansonia


Loaloa- Mangofly/Deerfly/Chrysops fly


O.volvulus- Simullium fly/ Black fly


M.ozzardi-Cullicoides


M.perstans-Cullicoides

Give the vectors for each microfilariae

W.bancrofti- SHEATED; nuclei NOT extending to tip


B.malayi- SHEATED; TWO nuclei at terminal


Loaloa- SHEATED; nuclei EXTENDS to tip


O.volvolus- UNSHEATED; nuclei NOT Extending to tip


M.ozzardi- UNSHEATED; nuclei NOT extending to tip


M.perstans- UNSHEATED; nuclei EXTENDS To tip


Note the Sheath & Presence of nuclei for each filaria

*W.bancrofti- NOCTURNAL (8:00PM-2:00AM or 10:00pm-4:00am)


*B.malayi- NOCTURNAL-SUBPERIODIC (Day or night much at night)


*Loaloa- DiURNAL (Day time 11:00am-1:00pm)


*O.volvulus- DOES NOT TRAVEL TO BLOOD


*M.ozzardi- NO PERIODICITY (Any time)


*M.perstans- NO PERIODICITY

Periodicity of each microfilaria?

KNOTT'S CONC. TECHNIQUE


Rgt: 2 ml formalin 8-10ml blood



Gold standard:ELISA

Concentration technique used to detect filariasis?reagents?



Gold standard to dx filariasis?

Visceral larva migrans: T.catis & T.canis


Cutaneous larva migrans (Creeping eruption): A.duodenale & A.caninum

What causes these?


Visceral larva migrans?


Cutaneous larva migrans?

BLOOD FLUKES OTHER FLUKE


-Cercaria has -Cercaria has


Forked tail straight tail


-No Redia -Has redia


-1 IH -2 IH


-Cercaria is -Metacercaria


Infective form is infective form


-Non operculated- operculated


Egg egg


-non-hermaph -hermaphroditic


roditic

Differentiate blood flukes and other flukes

S.japonicum(rbc may attach to shell) : minute/indistinct lateral knob


S.mansoni: lateral spine


S.haematobium: terminal spine

Differentiate the blood flukes

S.intercalatum- like S.haematobium, terminal spine with equitorial bulge (like shoulder) and shed in feces



S.mekongi- like S.japonicum

The schistosoma look alike?

S.japonicum - Oncomelania


S.mansoni- Biomphalaria, Planorbis, Tropicorbis


S.mansoni- Bulinus, Physopsis

Intermediate hosts of the blood flukes?

Eggs-> miracidium develops in eggs -> contact w/ H20 miracidium (with cilia) swims in water -> infects IH(Appropriate Snail) -> in snail miracidium becomes sporocyst -> becomes cercaria -> penetrates man skin causing swimmer's itch -> cercaria looses tail -> becomes schistosomule -> infects site whilr travelling causes Katayama fever

Life cycle of schistosoma?

LEAF SHAPED

Shape of Other flukes? (ALL except schistosoma)

Gynecophoric canal- "SCHIST"

Male schostosoma has this to hold the females duting copulation?

E.ilocanum

Fluke that has oral sucker with spine?

P.westermani

Fluke with coffee bean shape? And paralobule testes?

F.hepatica F.buski


-Cephalic cone -No cephalic


(Shoulder) cone


-intestinal ceca, -intestinal


Testes & ovaries ceca is


Branched/Dendritic unbranched

Differentiate F.hepatic & F. Buski since they are so alike

H.heterophyes

With 3rd sucker so called as "genital sucker"

C.sinensis O.felineus


Testes: Dendritic Lobed


Vitilaria: Granular Transverse

Differentiate C.sinensis & O.felineus?

E.ilocanum

Require two snails as a host?

P.westermani: (1st)Brotia asperata (2nd) Fresh Crabs


E.ilocanum: (1st) Gyrolus (2nd) another snail


F.hepatica: (1st)Lymnea philippinensis (2nd) Fresh H20 vegetation


F.buski: (1st) segmentia hyputis


(2nd) Fresh H2O vegetation


C.sinensi,O.felineus,M.yokogawai, H.heterophyes : (1st) Snail (2nd) Fishes



Generally: (1st IH) Snail (2nd IH) others

Intermediate host of other flukes

Eggs-> Miracidium-> contact w/H2O-> miracidium penetrates snail (1st IH) -> becomes sporocyst -> REDIA -> Cercaria (straight tail) -> leaves snail-> infects (2nd IH) -> becomes Metacercaria -> ingested by man (DH)

Life cycle of other flukes

C.sinensis

Resembles "OLD FASHIONED ELECTRIC BULB" ova

Halzoun

An arabic term for suffocation may happen due to lodgement of worms in the pharynx

F.hepatica

Causes liver rot?

Purely hermaphroditic


Can live up to 25 yrs


Flat segmented ribbon like bodies

Characteristic of Cestodes?

Longest: D.latum


Shortest: E.granulosus

Longest & shortes cestode by length?

H.nana

Only tapeworm that could live and exist in one host?

Head/Scolex -For attachment


Neck- Region of growth it is where segments originate


Segment/proglottid

Parts of a tapeworm?(Cestodes) and their purpose or content

With W/out


-T.solium -T.saginata


-H.nana -H.diminuta


-D.caninum


-E. granulosus

Tapeworm scolex with and without hooklets?

Diphyllobotrium latum

Only tapeworm that is psedocyclophillidea & spatulste/spoon/diamond head?

Immature - undeveloped F & M reproductive organs


Mature- well developed reproductive organs (1 testes 2 ovaries 1 genital pore) with excemptions


Gravid/Ripe- contains egg filled uterus



Excemptions:


T.solium- 3rd ovary , Accessory Ovarian lobe


D.caninum- Double pore tapeworm/ Dog tapeworm

Types of segments / proglottids?

D.latum

Rosette uterus is found where?

T.solium: 8-12, 8-15


T.saginata: 15-30

Differentiate uterine branches of T.solium & T.saginata?

H.nana & H.diminuta

Sac like uterus?

E.granulosus

Loosely twisted or coiled uterus?

D.caninum

Vase shaped or pumpkin seed segment?

E.granulosus

Only tapeworm that doesn't cause intestinal infection?

Egg pocket

In gravid females uterus id divided each fragment is called?

D.latum - non operculated egg

All tapeworms have non operculated eggs except?

P.westermani

D.latum produces eggs similar to?

H.nana: Has polar thickenings & polar filaments extending from it



H.diminuta: Has polar thickenings & no polar filaments extension, "FRIED EGG APPEARANCE"

Differentiate H.nana & H.diminuta egg

Loaloa

Causes calibar swelling/ fugative swelling abd hard too catch? But doesn't cause blindness?

Schistosoma

Causes Katayama fever?

E.granulosus

Eggs that are NEVER SEEN in human feces

Adult-> Egg (Oncosphere) -> Cysticercus



Cysticercus solium = T.solium


Cysticercus saginata = T.bovis



Ingestion of OVA = CYSTICERCOSIS



Ingestion of CYSTICERCOID = TAENIASIS


Life cycle of Taenia? Disease they cause?

Egg -> Procercoid -> Plerocercoid



Ingestion of Procercoid : Sparganosis



Ingestion of Plerocercoid: Diphylobotriasis

Life cycle of D.latum? Disease they cause?

Coenurus

Larval form of multiceps multiceps?

What are the probable parasites found on urin, stool, blood etc?

Parasite located in different places