Osler - Cp - Blood Bank 1

Front 1

What antibody is good at agglutination?

Back 1

IgM is better than IgG

Front 2

What antibody is better at hemolyzing red cells?

Back 2

IgM is better than IgG

Front 3

Something that only reacts on immediate spin?

Back 3

Cold antibody

Front 4

What does a negative gel test look like?

Back 4

Cells in a button t the bottom of the microtube

Front 5

What does a positive gel test look like?

Back 5

Cells spread in varying degrees through the microtube

Front 6

What does a negative solid phase test look like?

Back 6

Cells in a button at the bottom of the microplate, because the indicator cells don't bind to the test RBC's on the microplate wall

Front 7

What does a positive solid phase test look like?

Back 7

Have cells spread in a carpet all along the microplate wall

Front 8

Indirect antiglobulin test?

Back 8

Uncoated red cells + antibody + AHG

Front 9

Direct antiglobulin test?

Back 9

Washed patient red cells + AHG

Front 10

What neutralizes ABO?

Back 10

Saliva (secretor)

Front 11

What neutralizes Lewis?

Back 11

Saliva (secretor for Lewis B)

Front 12

What neutralies P1

Back 12

Hydatid cyst fluid, Pigeon egg fluid

Front 13

What neutralizes Sd?

Back 13

Urine

Front 14

What neutralizes Chido, Rogers

Back 14

Serum

Front 15

Facts about warm reactive antibodies (5)

Back 15

IgG
Require Exposure
Cause HDN
Cause HTRs
Significant

Front 16

Facts about cold reactive antibodies (5)

Back 16

IgM
Naturally occuring
No HDN
No HTRs
Insignificant

ABO IS THE HUGE EXCEPTION

Front 17

Type 1 ABO chains?

Back 17

Glycoproteins

Front 18

Type 2 ABO Chains?

Back 18

Glycosphingolipids

Front 19

What gene allows a person to make A or B antigens in their saliva?

Back 19

Secretor gene (chr 19)

Front 20

The Se gene codes for what enzyme?

Back 20

fucosyltransferase (FUT2)

Front 21

Action of FUT2 on type 1 chains?

Back 21

Adds fucose to terminal galactose, H antigen on type I chains

Front 22

What does the H gene do?

Back 22

It's product adds fucose to terminal galactose of type II chains, which is the type II H Antigen

Front 23

Enzyme enhanced?

Back 23

ABO, Rh, KIdd

Front 24

Enzyme decreased?

Back 24

MNS, Duffy

Front 25

Enzyme unaffected?

Back 25

Kell

Front 26

Where are type I chains found?

Back 26

In secretions

Front 27

Where are type II chains found?

Back 27

Bound to cells

Front 28

What is the group A sugar added to the H antigen?

Back 28

N-acetylgalactosamine

Front 29

What is the group B sugar added to the H antigen?

Back 29

Galactose

Front 30

What are the relative amounts of H by blood group?

Back 30

O > A2 > B > A1 > A1B

Front 31

What agglutinates cells with abundant H antigen?

Back 31

Lectin of Ulex europaeus

Front 32

What lectin agglutinates A1?

Back 32

Dolichos biflorus

Front 33

What lectin has specificity for the N blood group?

Back 33

Vicea graminea

Front 34

What is the difference between A1 and A2 blood groups?

Back 34

A1 has 4 times more A antigen
Small % of A2 form anti-A1

Front 35

The antibodies in blood group O individuals are different how?

Back 35

Predominately IgG, not IgM like other groups.
IgG can cross the placenta, HDN in group O moms.

Front 36

Most common cause of HDN?

Back 36

ABO.. not Rh

Front 37

When is acquired B phenotype seen?

Back 37

In contact with enteric gram negatives: colon cancer, intestinal obstruction, gram negative sepsis

Front 38

How does forward grouping and reverse grouping look like with acquired B phenotype?

Back 38

Forward: Anti-A 4+, Anti B 1-2+
Reverse: A1 cells - 0, B cells 4+

They are true blood group A

Front 39

Pathophysiology of acquired B?

Back 39

Bacteria deacetylate GalNac exposing galactosamine, which cross reacts with reagent anti-B

Front 40

Lewis acts on what chains?

Back 40

Type I chains

Front 41

How is Lewis A made?

Back 41

Adds fucose to subterminal GlcNAc

Front 42

How is Lewis B made?

Back 42

The SE gene and Lewis gene compete. Secretor gene adds fucose, making H, and lewis gene adds its fucose.

Front 43

If your type I chain is acted by on by the lewis gene before the Se gene, what do you get?

Back 43

Lewis A, no H

Front 44

If your type I chain is acted on by the lewis gene AFTER the se gene, what do you get?

Back 44

Lewis B and H antigen

Front 45

Patients who are Se and Le type what for lewis A and B?

Back 45

Lewis a-b+ because the secretor gene is much better at adding its fucose

Front 46

Do lewis a- b+ patients make anti-lewis A

Back 46

No! they still have a small amount, they have the gene to make lewis A but are also secretors and make H first---lewis B

Front 47

Only people to make anti-lewis antibodies?

Back 47

a-b- patients

Front 48

Pregnancy has what effect on Lewis?

Back 48

Patients may become a-b- and have transient insignificant lewis antibodies

Front 49

What is the relationship of H. pylori to the lewis blood group?

Back 49

It may attach via the Lewis b antigen

Front 50

Auto-Anti-I association?

Back 50

Mycoplasma, cold agglutinin disease

Front 51

Auto-Anti-i association

Back 51

EBV

Front 52

What is the parvovirus B19 receptor?

Back 52

the P antigen

Front 53

Paroxysmal cold hemoglobinuria is associated with what blood group?

Back 53

Biphasic IgG with anti-P specificity
Classically associated with viral infections in children and syphilis
(Donath Landsteiner biphasic hemolysin)
Bind cold, hemolyse warm

Front 54

R1?

Back 54

DCe

Front 55

R2

Back 55

DcE

Front 56

R0

Back 56

Dce

Front 57

Rz

Back 57

DCE

Front 58

r'

Back 58

dCe

Front 59

r"

Back 59

dcE

Front 60

r (wiener)

Back 60

dce

Front 61

r^y

Back 61

dCE

Front 62

Frequency of Rh in whites and blacks?

Back 62

Whites: R1>r>R2>R0
Blacks: R0>r>R1>R2

Front 63

Weak D testing requirements?

Back 63

All D negative blood donors
Not required for D negative recipients

Front 64

Anti D in a D-positive person?

Back 64

Partial D

Front 65

Only patients who definitely need weak D testing?

Back 65

D-negative babies with D-negative moms (apparent)

Front 66

Fact: Roughly 80% of people will make an anti-D after 1 unit

Back 66

Just a fact to know.

Front 67

Red cells that are really D positive that don't agglutinate?

Back 67

Weak D

Front 68

Which is more common Jka or b?

Back 68

A is slightly more common

Front 69

What is unique about the IgG in Kidd abs?

Back 69

Good at fixing compliment

Front 70

Facts about Kidd (3)

Back 70

Delayed HTRs (flashlight in eyes)
Anamnestic response
Mild HDFN

Front 71

What carries M and N antigen?

Back 71

Glycophorin A

Front 72

What carries S s and U antigens?

Back 72

Glycophorin B

Front 73

what is more common s or S?

Back 73

little s

Front 74

Anti M and anti N , cold or warm?

Back 74

cold

Front 75

Anti S s and U, cold or warm

Back 75

warm, significant, IgG, exposure

Front 76

African americans are more likely to have what duffy phenotype?

Back 76

a-b- in 68%

Front 77

Duffy a-b- have what protection?

Back 77

vivax and knowlesi malaria infection

Front 78

K antigen expression in whites and AA?

Back 78

9% whites, 2% blacks

Front 79

What is the most common non-abo antibody after anti-D

Back 79

Anti-K