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10 Cards in this Set
- Front
- Back
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Biotechnology |
Application of biological systems to obtain a product |
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Recombinant DNA technology |
Procedures by which a fragment of DNA of one organism is incorporated into the genome of a different one |
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Restriction enzyme |
DNA cutting enzyme that cut specific fragments of DNA, leaving single stranded sticky ends |
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DNA gel electrophoresis |
Separation of DNA fragments based on their size. DNA fragments are subjected to an electrical current and move toward the positive pole. Smaller fragments move faster. |
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Nucleic acid hybridization |
A fragment of a single stranded nucleic acid can hybridize with another fragment that has a complementary sequence of nucleotides. |
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DNA sequencing |
Process in which exact sequence of nucleotides in a DNA segment is determined. |
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Polymerase chain reaction |
Technique by which small amount of specific DNA fragments can be amplified in vitro |
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PCR has 3 steps: |
Denaturation (separation of DNA strands), priming (primer attaches to strand of DNA), and extension (DNA polymerase extends molecule) |
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Vector |
DNA molecules into which a segment of foreign DNA can be incorporated. |
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Transgenic organisms |
Recombinant plants and animals altered by addition of genes from other organisms. |
