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15 Cards in this Set
- Front
- Back
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E. coli can be engineered to make insulin for purification and use in the treatment of diabetes because
A) It needs to regulate its cell-glucose level. B) It's an ancient gene that now has no function. C) The insulin gene was inserted into it. D) It picked up the insulin gene from another cell. |
C) The insulin gene was inserted into it.
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Different pieces of DNA representing the pieces of the a genome of an organism and is stored in bacterial cells are called a
A) Library. B) Clone. C) Vector. D) Southern blot. E) PCR. |
A) Library.
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A population of cells carrying a desired plasmid is called a
A) Library. B) Clone. C) Vector. D) Southern blot. E) PCR. |
B) Clone.
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A technique used to identify bacteria carrying a specific gene is
A) Southern blot. B) Shotgun sequencing. C) Transformation. D) Cloning. |
A) Southern blot.
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Which of the following techniques is used to alter one amino acid in protein?
A) Cloning B) PCR C) Restriction enzymes D) Selection E) Site-direct mutagenesis |
E) Site-direct mutagenesis
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A source of heat-stable DNA polymerase is
A) Agrobacterium tumefaciens. B) Thermus aquaticus. C) Saccharomyces cerevisiae. D) Bacillus thuringiensis. E) Pseudomonas. |
B) Thermus aquaticus.
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Which organism naturally possesses the Ti plasmid?
A) Agrobacterium tumefaciens B) Thermus aquaticus C) Saccharomyces cervisiae D) Bacillus thuringiensis E) Pseudomonas |
A) Agrobacterium tumefaciens
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Which organism degrades PCBs and has been engineered to produce BT toxin?
A) Agrobacterium tumefaciens B) Thermus aquaticus C) Saccharomyces cerevisiae D) Bacillus thuringiensis E) Pseudomonas |
E) Pseudomonas
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A eukaryote used in genetic engineering is
A) Agrobacterium tumefaciens. B) Thermus aquaticus. C) Saccharomyces cerevisiae. D) Bacillus thuringiensis. E) Pseudomonas. |
C) Saccharomyces cerevisiae.
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The purpose of using an antibiotic resistance gene on a plasmid used in genetic engineering makes
A) Replica plating possible. B) Direct selection possible. C) The recombinant cell dangerous. D) The recombinant cell unable to survive. E) All of the above. |
B) Direct selection possible.
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A specific gene can be directly inserted into a cell by all of the following except
A) Protoplast fusion. B) a gene gun. C) Microinjection. D) Electroporation. E) Agrobacterium. |
A) Protoplast fusion.
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PCR can be used to identify a bacterium because
A) The DNA primers are specific. B) DNA polymerase will replicate DNA. C) DNA can be electrophoresed. D) All cells have DNA. E) None of the above. |
A) The DNA primers are specific.
Feedback: The DNA primer is a specific sequence of DNA that will bind to complementary DNA. It will only bind to the DNA if it matches. So because the DNA primer is specific, it will be know from which bacteria the complementary DNA comes from that it binds to. See the discussion on PCR. |
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Restriction enzymes are
A) Bacterial enzymes that splice DNA. B) Bacterial enzymes that destroy phage DNA. C) Animal enzymes that splice RNA. D) Viral enzymes that destroy host DNA. |
B) Bacterial enzymes that destroy phage DNA.
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The best definition of biotechnology is
A) The development of genetic engineering. B) The use of living organisms to make desired products. C) Curing diseases. D) The use of microorganisms in sewage treatment. E) All of the above. |
B) The use of living organisms to make desired products.
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The necessary ingredients for DNA synthesis can be mixed together in a test tube. The DNA polymerase is from Thermus aquaticus and the template is from a human cell. The DNA synthesized would be most similar to
A) Human DNA. B) T. aquaticus DNA. C) A mixture of human and T. aquaticus DNA. D) Human RNA. E) T. aquaticus RNA. |
A) Human DNA.
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