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39 Cards in this Set

  • Front
  • Back
Generation time
Time it takes for bacteria to grow in optimal conditions
Generation time
The counting of the cells
Plate Count
directly couning on the plate
Optical Density
Counting based on how clear the fluid is
Direct Microscope count
Counting on the microscope
Growth Phases
Lag- Inital statge..Lots of division
Log- Acitivity during growth
Stationary- Producing at the same rate they are dying
Death- Nutrients decline, reproduce slower than they die.
Thermophiles
Like increased temo 45-80c.
mesophiles
Human body temps- 20-50c
Psychrophiles
Cold temps- burried under ice
Obligate Aerobe
Must have O2
Microaerophile
Likes just a little less O2 in the environment
Obligate Anaerobe
Does not like any O2 in the environement
Aerotolerant
Does not mind O2 in the environment
facultative Anaerobe
Doesnt mind O2 in the environment but perfers O2.
Capnophiles
Grows better with with more than normal Co2 in the environment. 5% more.
Why is O2 dangerous?
Due to SOD turning Co2 into H2O2. A catalase then turns it into H20 + O2 to make it safe again.
Acidophiles
Like PH level between 0-5
Neutrophiles
Like PH level between 5-8
Alkalinophiles
Like PH level between 8-12
Activity of H2O (AW0
Foods with high water concentrate will spoil faster due to increase bacteria growth
UV Light
required by some organisms to live.
UV safe or dangerous? WHY?
Dangerous due to the fact that it kills DNA
What is the hardest to kill?
Endospores
Basic Growth factors?
CHNOPS, K, Mg, Fe, Ca, Mn, Zn, Cu
Activation energy
The amount of energy needed to bring molecules together to initate reactions.
Concentration
the # of enzymes per reaction impacts the time.
PH Concentration
Bell Cure.... the abily to have a range with the PH
temp Concentration
Curve with a Drop due to proteins being denatured at a certain temp
Allosteric regulation
how it physically affects the enzymes ability to work.
Anabolism
Building of new molecules
catabolism
Breaking down of molecules
Catabolism 3 big stages
Glycolysis, Kreb Cycle, and ETC
Substrate level phosphorylation
ATP is releasing a (P) and goes directly to the enzyme, enzyme can add that (P) to another molecule
carrier Molecules
NAD & FAD
Oxidation
Loss of electrons, gain of O2
reduction
Storing energy gain of electrons
Use of NAD & FAD
to break up glucose molecules for energy
Oxidation formula of NAD & FAD
NADH & FADH2
Where does cellular respiration happen?
Glycolysis happens in Cytoplasm