The Effect Of Temperature On The Activity Of An Enzyme

In this lab we learned the basics of enzymes and how they work. We were able to perform a quantitative assay of the activity of an enzyme in a tissue extract using a spectrophotometer. Also, we had to organize the data that was provided into tables and graphs so we could have the ability to test a few hypotheses. For example, we tested the whether the rate of the reaction was influenced by enzyme concentration, whether the activity of the enzyme was influenced by temperature and also too see if the activity of the enzyme was influenced by the pH of the solution, which you will then see the results on the graphs and charts that I provided. In figure one we show results of the trial run from the enzyme activity where the absorbance was at 500nm. …show more content…
Now, the fourth figure provides information based on the reaction rate vs. time. Out of all of the figures provided I think this is the one that actually shows drastic change. For example, we are able to see how the reaction rate vs. temperature starts off high and increase gradually for a little bit and then it decreases drastically. It seems as the temperature increases the reaction rate slows down each time. Then, in figure five I am able to provide information regarding the absorbance vs. time for pH. In this figure we are able to see that all of the absorbance rates start at the same level and then gradually increase, except for the one with the level of pH 9. PH 9 is the only rate in this figure that increases and then decreases drastically only to increase again. This can show us that although a couple of absorbance levels might drop but will increase again. Lastly, in figure 6 I am able to provide information regarding the reaction rate vs. pH. Both the graph and chart show us that the reaction rate increases rapidly at first and then it begins to gradually