Micro Lab Practical

Front 1

These enzymes excreted outside cell breakdown cell walls and are called

Back 1

exoenzymes

Front 2

Some enzymes assisted in reactions by

Back 2

coenzymes

Front 3

bacteria obtain energy by

Back 3

respiration and fermenatation

Front 4

Shuttling down of o2 down electron transport chain involvign cytochromes facilitaties movement of protons to outside wall is called

Back 4

oxidative phosphorylation

Front 5

In Oxidative phosphorlation ________ is converted to _____________

Back 5

O2

water

Front 6

In _________breakdown orgainic molecules to Alcohols, aldehydes acids and gases

Back 6

fermentation

Front 7

This test determines if bacteria capable of carrying out various fermentation reactions

Back 7

Fermentation tests

Front 8

This test determines if bacteria carry out respirator y metoabolism

Back 8

Oxidative

Front 9

This test determines between two modes of metabolism and ascertain if organism is oxidative, fermentative or capable of both kinds of metabolism

Back 9

Glucose

Front 10

The fermentation test used for mixed acid fermentation is

Back 10

Methyl Red

Front 11

The fermentation test used for butandiol fermentation is

Back 11

Vogus Proskauer

Front 12

If glucose test determines bacteria is oxidative and not capable of fermenting surgrs then bacteria ______ be identified by fermenation tests

Back 12

cannot

Front 13

what three durham tubes are used

Back 13

glucose

lactose

manitol

Front 14

Durham tubes are consider _______ tests

Back 14

fermentation

Front 15

To determine mixed acid in a bacteria after positive Glucose one would use

Back 15

Methyl red

Front 16

To determine if there was butanediol fermentation after positive glucose one would use

Back 16

Voges proskauer tes

Front 17

If either MR or VP positive other test should be

Back 17

negative

Front 18

to test bacteria to see if they use _______ as sole carbon source you would peform the _______ test

Back 18

citrate

citrate

Front 19

Organism that debrated citrate must use the medias ammonium salts which causes media to become _________ turning the media _______

Back 19

alkaline

blue

Front 20

the oxidase tests determines if bacteria use _____ as terminal electron acceptor or can use_____and other sources

Back 20

O2

O2

Front 21

Oxidase test turns media a blue color if

Back 21

O2 primary acceptor

Front 22

The catalase test determines if bacteria contain enzyme catalase which allows for __________. This test is useful as ____________ and __________ do not have the enzyme which can damage bacteria.

Back 22

reduction of hydrogen peroxide

Strict Aerobes and Aerotolerant

Front 23

bacteria that reduce nitrates is determined by end product _______ or _______.

Back 23

gas or nitrate

Front 24

Bacteria that use nitrate respiration are a a specific _________ respiration

Back 24

anerobic

Front 25

Bacteria that uses starch produce ______ that degrade amylases that degrade the starch molecule into molecules of maltose, glucose, and dextrin

Back 25

amalyse

Front 26

starch degradation is used by adding _______ complexes

Back 26

Iodine

Front 27

The casein test determines if bacteria produce ______ which degrade protein molecules such as ______ into peptides and amino acids

Back 27

proteases

casein

Front 28

_______ this is multiple test medium that detects the production of ______ and ______ and determine if organism is ________ or _____.

Back 28

Indole

Hydrogen sulfide

motile

non motile

Front 29

Indole is the result of the breakdown of _________

Back 29

tryptophan

Front 30

When Kovac's reagent added to sims tube turns media black it means bacteria degraded ______ causing _____________ to be released which will combine with ferrous salts to produce a _______ preticipate in the tube.

Back 30

cysteine

hydrogen sulfate

black

Front 31

This agar allows release of fatty acids from Tributyrin via lipase activity which results in lower PH of ago to produce dark blue participate

Back 31

Spirt Blue Agar

Front 32

This medium is used to test for fat hydrolysis

Back 32

Spirit blue

Front 33

This is a differential media that allows one to distinguish different types of bacteria

Back 33

EMB

Front 34

Media is _______ to for 15 min at 121C to ensure sterile

Back 34

autoclaved

Front 35

If media needs vitamin and cannot be autoclaved it is _________

Back 35

filtered

Front 36

compounds that kill or inhibit microorganisms

Back 36

Antimicobials

Front 37

These are antimicrobials, usually of low moleccular weight, produced by microorganisms that inhibit or kill other organisms

Back 37

Antibiotics

Front 38

Antibiotics that are chemically altered are called

Back 38

Semi-synthetics

Front 39

The _________ method is used to determine the sensitivity of resistance of a bacterium

Back 39

Kirby Bauer

Front 40

How is kirby bauer method done

Back 40

bacteria streaked onto Mueller-hinton medium and then paper discs containing different antibiotics

Front 41

What is the no growth zone found on the Kirby Bauer plates called

Back 41

Zone of inhibition

Front 42

This is produced by some gram - bacteria, is a waste product of animal metabolism and is used to distinguish different bacterias.

Back 42

Urea

Front 43

Urea production causes ph to become _______ and color of tube to turn ______.

Back 43

Alkaline

pink

Front 44

This ensures that no contaminating microorganisms are introduced into cultures or culture materials and microbiologist is not contaminated by cultures that are being manipulated

Back 44

aseptic technique

Front 45

first step in aseptic technique

Back 45

work area disenfection

Front 46

Broth to broth transfer method

Back 46

heat loop, tube mouth flamed, culture innoculated, tube mouth flamed, cap replaced,

Loop sterilized and then work area

Front 47

Fungi secrete ______ that break down poly saccahrides

Back 47

exoenzymes

Front 48

These are fungi that form colonies composed of microscopic, rounded, intertwining fialments called ________. Two structures of fungi _____

and _______.

Back 48

Hyphae

Septate

coenocytic

Front 49

Yeasts do not form _____. The primary mode of reporduction is the ________

Back 49

Hyphae

Saccharomyces budding

Front 50

When yeast cells do not separate they are called

Back 50

pseudohyphae

Front 51

Some pathogenic fungi are

Back 51

dimorphic

Front 52

Fungi form symbiotic association with algae and cyanobacteria called

Back 52

lichens

Front 53

_______ account for 98% of fungal partner

Back 53

Ascomycetes