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52 Cards in this Set
- Front
- Back
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What does the ELISA for HIV detect |
HIV antibodies |
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In the ELISA for HIV, what will indicate a positive test result |
Blue color in the wells |
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Complete name for HIV |
HUMAN IMMUNODEFICIENCY VIRUS |
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ELISA is an acronym that means |
ENZYME LIMITED IMMUNOSORBENT ASSAY |
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What end result would u have in the ELISA for HIV if u forgot the secondary antibody |
Possible false negative |
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What result would u see in the negative control wells if u failed to wash them |
False positive |
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Components in order for ELISA test procedure |
Antigen, pt sample, second antibody, enzyme substrate |
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What do we call inanimate objects that are contaminated w/ pathogenic microbes |
Fomite |
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What is term for first pt to become ill in an epidemic |
Index case |
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Name 2 ways infectious diseases are transmitted |
Ingested and inhalation |
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Define common source epidemic |
When many people are infected from one common source |
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Define propagated transmission |
When an infection is transmitted by person to person |
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Define disinfectant |
Germicidal agent used on inanimate object |
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Define antiseptic |
Germicidal agent used on living tissues |
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Define Use-Dilution |
Standard procedure used to measure the effectiveness of disinfectants |
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In the disinfectant lab why do u put nails in water first |
-do same thing to controls that u do to other tubes - just to see if water alone does anything |
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In disinfectant lab why do u put nails on paper |
To dry culture on the nails |
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LB |
Help heal and give nutrients |
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Arabinose |
Makes it glow |
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Typically E-coli will die in presence of |
Penicillin |
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Define bacteria transformation |
When bacteria picks up DNA from environment |
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What is the process by which bacterial cells pick up DNA from the environment and make use of the genes it carries |
Transformation |
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What must be done to the Ecoli cells in order to allow transformation |
Use cacL2 transformation solution and heat shock for it |
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What is commercial name of the plasmid used in the transformation kit |
pGLo plasmid |
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araC |
Regulatory molecule for arabinose promoter |
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bla |
Ampicillin resistance |
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GFP |
Provides glow under fluorescent light |
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Ori |
Necessary for DNA replication |
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On which plate is it possible to see Ecoli cells that HAVE taken up the vector |
LB, LB + Amp, LB+ Amp +Ara |
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On which plate can u see glowing colonies |
LB + Amp + Ara |
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On which plate can u see cells that HAVE NOT taken up the vector |
LB |
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Histoplasma Capsulatum- very common in Ohio River Valley |
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Saccharomyces cereviseae- called baker's yeast and brewer's yeast |
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Trichinella spiralis- infections are associated w/ numerous eosinophilis in the blood |
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Nacator americanus head- new world hookworm |
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Giardia lamblia trophozoite- causes diarrhea |
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Penicillium notatum- produces antibiotic |
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Tick- an arachnid, not an insect |
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Schistosoma mansoni- adult female lives in a groove along the adult male's body |
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Toxoplasma gondii trophozoites- oocyst & pseudocyst stages infect humans |
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Candida albicans- forms pseudohyphae |
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Ascaris lumbricoides- adults often measure about 30 cm long |
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Rhizopus- produces anchoring rhizoids |
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Flea- laterally flattened, wingless insect |
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T. Saginata- pigs and cattle are hosts |
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Plasmodium falciparum- 4 species infect humans |
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Enterobius vermicularis- causes most common worm infection of children in US |
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Lice- dorsally-ventrally flattened, wingless insect |
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Trichomonas vaginalis- cause STD |
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Pneumocystis jiroveci- causes most frequent opportunistic infection of AIDS pt |
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Incidence rate formula |
Have to write cases/people |
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Point prevalence formula |
Have to write cases/people |
