Micro Lab Final

Front 1

What test or characteristic will differentiate between this set of organisms?

Proteus sp. versus Salmonella typhi

Back 1

• Urea broth test
• Proteus sp. = positive results, pink color, utilizes urease and produces ammonia
• Salmonella typhi = negative results

Front 2

What test or characteristic will differentiate between this set of organisms?

Bacillus sp. versus Clostridium sp.

Back 2

• Trypticase Soy Agar
• Bacillus sp. = facultative aerobe
• Clostridium sp. = obligate anearobe

Front 3

What test or characteristic will differentiate between this set of organisms?

Escherichia sp. versus Klebsiella sp.

Back 3

• Methyl Red test
  
• Escherichia sp. =positive results for mixed acid fermentation
  
• Klebsiella sp. =negative results

Front 4

What test or characteristic will differentiate between this set of organisms?

Proteus vulgaris versus Proteus mirabilis

Back 4

• Vogues-Proskauer test
• Proteus vulgaris =negative results, no acetoin.
• Proteus mirabilis =positive results, red color change, has acetoin.

Front 5

What test or characteristic will differentiate between this set of organisms?

Escherichia coli versus Citrobacter freundii

Back 5

• Simmons Citrate test
• Escherichia coli =negative, no change
• Citrobacter freundii =positive results, blue color change, utilizes citrate

Front 6

What test or characteristic will differentiate between this set of organisms?

Streptococcus faecalis versus Staphylococcus aureaus

Back 6

• Catalase test
• Streptococcus faecalis =negative results, no catalase production
  
• Staphylococcus aureaus =positive results, produces catalase
  

Front 7

1. Give genus name
   
2. Phylum
3. Disease caused
   
4. How it's acquired
   

Back 7

1. Entamoeba histolytica

E n t a m o e b a

h i s t o l y t i c a

2. Sarcodina (amoebas)

3. Amebiasis

4. passed in feces; common in people who live in tropical areas with poor sanitary conditions

Front 8

1. Give genus name
2. Phylum
3. Disease caused
4. How it's acquired

Back 8

1. Entamoeba coli

E n t a m o e b a

c o l i

2. Sarcodina (amoebas)

3. N/A

4. passed in feces; common in people who live in tropical areas with poor sanitary conditions

Front 9

1. Give genus name
2. Phylum
3. Disease caused
4. How it's acquired
   

Back 9

1. Acanthamoeba

A c a n t h a m o e b a

2. Sarcodina (amoebas)

3. causes three main types of illness involving: --the eye (Acanthamoeba keratitis),

--the brain and spinal cord (Granulomatous Encephalitis), and infections that can spread throughout the

--entire body (disseminated infection)

4. Acanthamoeba can enter the skin through a cut, wound, or through the nostrils.

Once inside the body, amoeba can spread through the bloodstream to other parts of the body.

Acanthamoeba can also enter the eye, where it causes an infection of the cornea.

Front 10

1. Give genus name
2. Phylum
3. Disease caused
4. How it's acquired
   

Back 10

1. Giardia lamblia

G i a r d i a

l a m b l i a

2. Mastigophora

3. Giardiasis (diarrhea)

4. The main route to infection is ingestion of untreated sewage and contaminated natural waters.

Front 11

1. Give genus name
2. Phylum
3. Disease caused
4. How it's acquired
   

Back 11

1. Trichomonas vaginalis

T r i c h o m o n a s

v a g i n a l i s

2. Archezoa

3. Trichomoniasis

4. The parasite is passed from an infected person to an uninfected person during sex.

Front 12

1. Give genus name
2. Phylum
3. Disease caused
4. How it's acquired
   

Back 12

1. Trypanosoma gambiense

T r y p a n o s o m a

g a m b i e n s e

2. Euglenozoa

3. African sleeping sickness

4. the bite of an infected tsetse fly

--Mother-to-child infection

--sexual contact

Front 13

1. Give genus name
2. Phylum
3. Disease caused
4. How it's acquired
   

Back 13

1. Balantidium coli

B a l a n t i d i u m

c o l i

2. Ciliata

3. balantidiasis

4. Ingestion of contaminated water and food. Fecal to oral transmission is most common.

Front 14

1. Give genus name
2. Phylum
3. Disease caused
4. How it's acquired
   

Back 14

1. Plasmodium malariae

P l a s m o d i u m

m a l a r i a e

2. Sporozoa

3. Malaria

4. Spread to people through the bites of infected female Anopheles mosquitoes

Front 15

1. Give genus name
2. Taxa
3. Disease caused
4. How it's acquired
   

Back 15

1. Fasciola hepatica (Liver fluke)

F a s c i o l a

h e p a t i c a

2. Trematodes

3. Fascioliasis

4. People usually become infected by eating raw watercress or other water plants contaminated with immature parasite larvae.

Front 16

Helminths include members of the following taxa: cestodes are...

Back 16

tapeworms

Front 17

Helminths include members of the following taxa: nematodes are...

Back 17

roundworms

Front 18

Helminths include members of the following taxa: trematodes are...

Back 18

flukes

Front 19

1. Give genus name
2. Taxa
3. Disease caused
4. How it's acquired
   

**BE ABLE TO DRAW

Back 19

1. Taenia solium (pork worm)

T a e n i a

s o l i u m

2. Cestode

3. Taeniasis

4. Humans can become infected with these tapeworms by eating raw or undercooked pork

Front 20

1. Give genus name
2. Taxa
3. Disease caused
4. How it's acquired
   

Back 20

1. Trichinella spiralis (pork worm)

T r i c h i n e l l a

s p i r a l i s

2. Nematode

3. Trichinosis

4. Consuming under cooked pork products

Front 21

1. Give genus name
2. Taxa
3. Disease caused
4. How it's acquired
   

Back 21

1. Schistosoma mansoni

S c h i s t o s o m a

m a n s o n i

2. Trematode

3. Schistosomiasis

4. Infection occurs when your skin comes in contact with contaminated freshwater in which certain types of snails that carry schistosomes are living.

Front 22

Identify

Back 22

Aspergillus Niger

Front 23

Identify

Back 23

Aspergillus fumigatus

Front 24

Identify

Back 24

Penicillium

Front 25

Identify

Back 25

Rhizopus

Front 26

Mannitol Salt Agar (MSA)

1. Substrate
2. Enzyme
3. Product
4. Indicator
5. Change
6. Inhibitor
7. Grows

Back 26

1. Mannitol
2. Mannitol dehydrogenase
3. mixed organic acids
4. phenol red
5. Yellow below pH 6
6. Gram (-)
7. Gram (+)

Front 27

Eosin Methylene Blue Agar (EMB)

1. Substrate
2. Enzyme
3. Product
4. Indicator
5. Change
6. Inhibitor
7. Grows

Back 27

1. Lactose
2. Lactase
3. mixed organic acids
4. Methylene blue Eosin
5. metallic green sheen; Pink colonies with dark center
6. Gram (+)
7. Gram (-)

Front 28

MacConkey Agar (MAC)

1. Substrate
2. Enzyme
3. Product
4. Indicator
5. Change
6. Inhibitor
7. Grows
   

Back 28

1. Lactose
2. Lactase
3. mixed organic acids
4. Neutral Red
5. Fuschia Pink below pH 6
6. Gram (+)
7. Gram (-)

Front 29

Hekteon Agar (HEK)

1. Substrate
2. Enzyme
3. Product
4. Indicator
5. Change
6. Inhibitor
7. Grows
   

Back 29

1. Lactose
2. Lactase
3. mixed organic acids
4. Bromthymol Blue
5. greenish yellow when pH 6.5
6. N/A
7. N/A

Front 30

Phenol Red Broths

1. Substrate
2. Enzyme
3. Product
4. Indicator
5. Change
6. Inhibitor

Back 30

1. Glucose; Lactose; sucrose
2. Glucose-6; Lactase; sucrase
3. mixed organic acids
4. phenol red
5. yellow below pH 6; Purple above pH 7.5; Red at pH 7

6. Distinguish between fermenters / non of sugar

Front 31

Methyl Red

1. Substrate

2. Enzyme

3. Product

4. Indicator

5. Change

Back 31

1. Glucose
2. Glucose-6
3. mixed organic acids
4. Methyl red reagent
5. pH below 6.5 red; above 6.6 yellow

Front 32

Voges-Proskauer broth

1. Substrate
2. Enzyme
3. Product
4. Indicator
5. Change

Back 32

1. Glucose
2. Glucose-6
3. acetoin
4. Barrits A/B reagent
5. change means presents of acetoin

Front 33

Nitrate Broth

1. Substrate
2. Enzyme
3. Product

Back 33

1. nitrate
2. nitrate reductase
3. nitrite or ammonia

Front 34

Simmons Citrate Agar

1. Substrate
2. Enzyme
3. Product
4. Indicator
5. Change

Back 34

1. Citrate
2. citrate reductase
3. ammonia
4. bromthymol blue
5. changes to blue if ammonia is present

Front 35

Starch Agar

1. Substrate
2. Enzyme
3. Product
4. Indicator
5. Change

Back 35

1. Starch
2. amylase
3. glucose
4. Iodine reagent
5. purple/black color

Front 36

Urea Broth

1. Substrate
2. Enzyme
3. Product
4. Indicator
5. Change

Back 36

1. Urea
2. urease
3. ammonia
4. phenol re
5. purple if pH is 8 or above

Front 37

Skim Milk Agar

1. Substrate
2. Enzyme
3. Product
4. Indicator
5. Change
   

Back 37

1. Casein
2. caseinase
3. free amino acids
4. N/A
5. clear zone if amino acids are present

Front 38

Gelatin tube

1. Substrate
2. Enzyme
3. Product
4. Indicator
5. Change
   

Back 38

1. Gelatin
2. gelatinase
3. free amino acids
4. N/A
5. change to liquified

Front 39

Sulfur Indole Motility (SIM)

1. Substrate
2. Enzyme
3. Product
4. Indicator
5. Change

Back 39

1. Cysteine
2. cysteine desulfurase
3. Hydrogen sulfide (H2S)
4. ferric sulfate (FeSO4)
5. reacts to produce FeS color change to black

Front 40

Triple Sugar Iron Agar

1. Substrate
2. Enzyme
3. Product
4. Indicator
5. Change
   

Back 40

1. Glucose; Lactose
2. Glucose-6; Lactase
3. Mixed organic acids
4. N/A
5. test for enterobacteriacease, pseudomonads, Bacillu

Front 41

A disk diffusion test where small antimicrobic-impregnated paper disks are placed in a petri dish with pathogenic microorganisms then incubated. The antimicrobic drug moves through the agar. If the organism is susceptible to the drug, a clear zone will appear around the disk. The bigger the zone, the more effective.

This test is called ______ _____ _______.

Back 41

Kirby-Bauer Method

Front 42

If you see a streak on an EMB plate with a metallic green shean, this indicates what?

Back 42

The presence of Escherichia coli