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17 Cards in this Set
- Front
- Back
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Gram stain |
Positive- purple Negative- red crystal violet - red counterstain |
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Endospore |
white shining back light in cell |
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acid fast |
lots of mycolic acid |
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oxidase |
oxidise phenylene diamine, purple paper, tetramethyl-para- diphenylamine |
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catalase |
gas bubbles from breakdown of hydrogen peroxide |
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MTM |
flagella so movement from stab |
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Oxidation or fermentation of glucose |
only green if it cant ferment yellow is normal for acid production |
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acid and gas from glucose |
ferment glucose turn it yellow gas from fermentation captured |
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Mannitol salt agar MSA |
NaCL- selects for halotolerant mannitol fermenters make it yellow |
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Triple sugar iron agar TSI |
1% sucrose and 1% lactose so if good growth, them. black h2s acid yellow alkaline red,pink |
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MRVP |
Gram - red+ yellow - ferment glucose VP red band at top produces acetic acid - converted to acetylmethylcarbinol |
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Eosin methylene blue agar EMB |
lactose fermentation- causes acid, therefore pink if little lots of acid = metallic sheen |
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MacConkey Agar |
Lactose- pink if positive for fermentation |
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citrate |
citrase breaks down citrate into, oxalo acid and acetic. makes it Prussian blue for co2 production for positive |
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urease |
will go hot pink if positive |
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starch hydrolysis |
amylase,maltase- iodine leaves clearing hole- blue = nothing |
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anaerobic/aerobic |
paraffin, growth shows anerobic |
