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51 Cards in this Set
- Front
- Back
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how to calculate CFU/gram of food
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CFU counted on plate times reciprocal of dilution
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how many CFU's would be a countable plate
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30-300
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organisms that grow -5 to 20 C
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psychrophiles
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organisms that grow 20-50C
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mesophiles
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organisms that grow 50-80C
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thermophiles
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organisms that grow over 80C
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hypothermophiles
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requires high salt concentration
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halophiles
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agar with designated salt concentration
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nutrient agar
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requires a lower concentration of oxygen
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microaerophiles
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indifferent to oxygen, can grow in O2 but doesn't use O2 as a terminal electron acceptor
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aerotolerant (Streptococcus)
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Kirby-Bauer Antibiotic Susceptibility Test uses what kind of agar
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Mueller Hinton agar
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standardized inoculum for Kirby-Bauer antibiotic test
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McFarland 0.5 turbidity
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can be applied to tissue, doesn't kill endospores
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antiseptics (alcohol)
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chemical for inanimate surfaces
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disinfectants (sterilants, sporocides)
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results for blood agar plate
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beta, alpha, gamma
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is SBA differential and selective
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differential for ability to lyse RBC
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mannitol salt agar positive vs negative result
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positive is yellow, negative is red
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MSA differential and selective?
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differential for ability to ferment mannitol and selective for Staph. species
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MacConkey Agar differential and selective?
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differential based on ability to ferment lactose, selective against Gram + because of bile salts and crystal violet
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positive and negative results for MacConkey Agar
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lactose positive: pink colony, negative: no color
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catalase differential and selective
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differential for catalase to break down hydrogen peroxide to water and oxygen
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oxidase differential and selective
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differential for presence of cytochrome
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results + and - for oxidase test
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positive: purple color on cotton swab, negative: yellow
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3% KOH used for what and what results
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confirms Gram stain, positive: viscious stringy appearance (Gram -), negative: no results is Gram +
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coagulase test for what species and + and - results
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tests fibrin clot formation in Staph.
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2 asexual spores of molds
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sporangiospores, conidia
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3 sexual spores of molds
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basidiospores, ascospores, zygospores
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asexual spore of yeast
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blastospore or bud
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type of agar used to enhance growth of fungi, (pH? dextrose?)
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sabouraud dextrose agar, low pH high dextrose
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what stuff do you add in scotch tape prep for fungi
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lactophenol cotton blue
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area of clearing where phage have infected and killed bacteria
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plaque
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4 phage types
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T 1, 2, 3, 5
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breaks down peptidoglycan in cell wall
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lysozyme
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colors of Anti-A, B and D serum
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blue, yellow, white
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SIM stand for
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sulfide indole motility
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indole reagent aka
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Kovac's reagent
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+ and - results of indole production
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+ is red, - is no color change
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sulfide + and - results
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+ blackening along stab line
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citrate test for what and + and - results
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test ability to use citrate as sole carbon source, + is intense blue, - is no change and remains green
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purple broth lactose test what and + and - results
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ability to ferment lactose, + is yellow, - is no color change
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urea test what enzyme which does what and what is + results
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enzyme urease splits urea to form ammonia, postiive is red-pink color
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lysine decarboxylase test what
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ability to decarboxylate lysine to cadaverine and CO2
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+ and - results of lysine decarboxylase
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+ is purple (cadaverine produced), - is bright clear yellow (dextrose only)
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fermenation of dextrose results
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oxidative: yellow in open, fermentative, yellow in both, neither: blue/green in both
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sterile mineral oil goes in what two tests
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lysine decarboxylase and dextrose
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MRVP stand for
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methyl red voges-proskauer
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produce and maintin stable acid end products from glucose fermenation
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MR
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produce neutral end product (acetoin)
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VP
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two reagents used in VP
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agent A is alpha-naphtol in absolute alchohol, agent B is 40% KOH
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results + and - of VP
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+ is red in 15 minutes, - is no color/coppor
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+ and - for MR
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+ is red immediately, - is yellow color
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