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68 Cards in this Set

  • Front
  • Back
The three basic type of hemolysis for Streptococcus species are:
alpha (incomplete) hemolysis - greenish color
beta (complete) hemolysis - transparent
gamma (no) hemolysis - no effect
Streptococcus Group A Streptococcus or Streptococcus pyogenes

hemolysis type:__________
catalase reaction:_________
gram POSITIVE cocci in:__________
TAXO-A:__________
PYR:__________
Streptococcus Group A Streptococcus or Streptococcus pyogenes

hemolysis type: beta
catalase reaction: negative (-) [No bubble formation after adding H2O2]
gram POSITIVE cocci in: chains
TAXO-A: positive (+)
PYR: positive (+)
Streptococci pneumoniae

hemolysis type:__________
catalase reaction:__________
gram POSITIVE cocci in:__________
bile esculin:__________
Optochin disk or TAXO-P:__________
Bile solubility:__________
Streptococci pneumoniae

hemolysis type: alpha
catalase reaction: negative (-) [No bubble formation after adding H2O2]
gram POSITIVE cocci in: chains
bile esculin: negative (-) [no black pigmentation]
Optochin disk or TAXO-P: positive (+) [susceptible]
Bile solubility: positive (+)
Streptococcus Group B or Stretococcus agalactiae

hemolysis type:__________
catalase reaction:__________
gram POSITIVE cocci in:__________
CAMP TEST:__________
Hippurate hydrolysis:__________
Streptococcus Group B or Stretococcus agalactiae

hemolysis type: beta
catalase reaction: negative (-) [No bubble formation after adding H2O2]
gram POSITIVE cocci in: chains
CAMP TEST: positive (+)
Hippurate hydrolysis: positive (+)
Streptococcus viridians

hemolysis type:__________
catalase reaction:__________
gram POSITIVE cocci in:__________
TAXO-P:__________
Bile solubility:__________
PYR:__________
Streptococcus viridians

hemolysis type: alpha
catalase reaction: negative (-) [No bubble formation after adding H2O2]
gram POSITIVE cocci in: chains
TAXO-P: negative (-) [resistant]
Bile solubility: negative (-) [insoluble]
PYR: negative (-)
Streptococcus Group D

hemolysis type:__________
catalase reaction:__________
gram POSITIVE cocci in:__________
TAXO-P:__________
Bile solubility:__________
PYR:__________
6.5% NaCl:__________
Bile esculin:__________
Streptococcus Group D

hemolysis type: alpha/gamma
catalase reaction: negative (-) [No bubble formation after adding H2O2]
gram POSITIVE cocci in: chains
TAXO-P: negative (-)
Bile solubility: negative (-)
PYR: negative (-)
6.5% NaCl: negative (-)
Bile esculin: postive (+) [black pigmentation]
Enterococcus spp./Group D streptococcus

hemolysis type:__________
catalase reaction:__________
gram POSITIVE cocci in:__________
TAXO-P:__________
Bile solubility:__________
PYR:__________
6.5% NaCl:__________
Bile esculin:__________
Enterococcus spp./Group D streptococcus

hemolysis type: alpha/gamma
catalase reaction: negative (-) [No bubble formation after adding H2O2]
gram POSITIVE cocci in: chains
TAXO-P: negative (-)
Bile solubility: postive (+)
PYR: postive (+)
6.5% NaCl: postive (+)
Bile esculin: postive (+) [black pigmentation]
What organism is the common cause of bacteria meningitis in neonates (newborns)?
Streptococcus Group B or agalactiae
What organism is the common cause of epidemic meningitis?
Neisseria meningtides
What organism is the common cause of bacteria meningitis in children?
Haemophilus influenzae
Haemophilus influenza

gram NEGATIVE:_________
oxidase:_________
catalase:_________
requires which factors:_________
grow only on which media:_________
Haemophilus influenza

gram NEGATIVE: coccobacilli
oxidase: positive (+)
catalase: positive (+) [bubble formation after adding H2O2]
requires which factors: x and v factors
grow only on which media: chocolate agar
Neisseria gonorrhea

gram NEGATIVE:_________
oxidase:_________
catalase:_________
grow only on which media:_________
utitilizes which sugar:__________
Neisseria gonorrhea

gram NEGATIVE: diplococci
oxidase: positive (+)
catalase: positive (+) [bubble formation after adding H2O2]
grow only on which media: chocolate agar, MTA (Modified Thayer Martin)
utitilizes which sugar: glucose only
What 2 organisms are used for satellism testing?
Haemophilus influenza
and
Staphylococcus aureus
Staphylococcus aureus

catalase test:__________
gram POSITIVE cocci in:__________
Slide Coagulase test:__________
Tube Coagulase test:__________
Mannitol salt agar:__________
Staphylococcus aureus

catalase test: positive (+) [bubble formation after adding H2O2]
gram POSITIVE cocci in: clusters
Slide Coagulase test: positive (+) [clumps]
Tube Coagulase test: positive (+) [clumps]
Mannitol salt agar: positive (+) [yellow colored colonies]
Staphylococcus saprophyticus

catalase test:__________
gram POSITIVE cocci in:__________
Slide Coagulase test:__________
Tube Coagulase test:__________
Novobiocin results:__________
Staphylococcus saprophyticus

catalase test: positive (+) [bubble formation after adding H2O2]
gram POSITIVE cocci in: clusters
Slide Coagulase test: negative (-) [clumps]
Tube Coagulase test: negative (-) [clumps]
Novobiocin results: resistant
Sheep blood agar plates or TSA-II (Tryptic Sol Agar) with 5% SB (Sheep's Blood) is used for which type of cultures:

a. __________
b. __________
c. __________
d. __________
e. __________
f. __________
g. __________
h. __________
a. throat cultures
b. sputum cultures
c. urine cultures
d. blood cultures
e. CSF (cerebrospinal fluid)cultures
f. wound cultures
g. genital cultures
h. ear/eye cultures
What does a TSI (Triple Sugar Iron Agar) medium contain?
(From top to bottom)
1) lactose, 2) surcose, 3) glucose, and Iron
Explain a A/A (acid on acid) TSI (Triple Sugar Iron Agar) medium.
Ferments lactose and/or sucrose and glucose turning the media all yellow
Explain a A/K (acid on alkaline) TSI (Triple Sugar Iron Agar) medium.
Ferments lactose and/or sucrose and does not ferment glucose, yellow slant and alkaline butt
Explain a K/A (alkaline on acid) TSI (Triple Sugar Iron Agar) medium.
Does not ferment lactose, may ferment sucrose and ferments glucose, alkaline slant and acid butt
Explain a K/K (alkaline on alkaline) TSI (Triple Sugar Iron Agar) medium.
does not ferment lactose, sucrose or glucose. Alkaline slant and alkaline butt
Explain a black colored TSI (Triple Sugar Iron Agar) medium.
produces H2S
MSA (Mannitol Salt Agar) is classified as which type of media?
selective and differntial
MSA (Mannitol Salt Agar) is used for the identification of which organism?
Staphylococcus aureus
Enrichment broth such as GN (gram-negative) broth or Selenite is used for cultures which __________ normal flora.
supresses
What test(s) can be used to identify Salmonella or Shigella species from other Enterobacteriaceae?
a. serological testing
b. bio-chemical testing
What does IMViC stand for and which type of organisms are they use to identify?
I - Indole test
M - Methyl red test
Vi - Voges-Proskauer
C - Citrate

Identifies: Enterobacteriaceae
List the 10 genera of Enterobacteriaceae.
1) enterobacteriaceae coli (E. coli)
2) citrobacter
3) klebsiella
4) enterobacter
5) serratia
6) proteus
7) providencia
8) salmonella
9) shigella
10) yersinia
What is the color apperance of Salmonella colonies on HE (Hektoen Enteric) plates?
black centered colonies witha green halo
What is the color apperance of Salmonella colonies on XLD (Xylose Lysine and Desoxychocolate) plates?
black centered colonies witha red halo
What is the color apperance of Salmonella colonies on MaConkey plates?
clear pink colonies
What is the color apperance of Shigella colonies on HE (Hektoen Enteric) plates?
clear green colonies
What is the color apperance of Shingella colonies on XLD (Xylose Lysine and Desoxychocolate) plates?
clear red colonies
What is the color apperance of Shingella colonies on MaConkey plates?
clear pink colonies
Salmonella spp., in addition to what they show on differnt plates, what will they will also produce?
H2S
Campylobacter jejuni isolated on a campy plate

catalase test:__________
oxidase reaction:__________
gram stain:__________
motility description:__________
Campylobacter jejuni isolated on a campy plate

catalase test: postive (+)
oxidase reaction: positive (+)
gram stain: gram negative rods, s shape, or seagull shape
motility description: darting motility
List 4 rapid methods used for the identification of Enterobacteriaceae.
1) enterotube II
2) API
3) Microscan
4) Vitek
UA culture grows as follow: TSA (Tryptic Sol Agar) >100 colonies (same), MAC-NG (MaConkey agar plate - no growth), Enterococcal >100 colonies (same) PYR+ report as:
cc >or= 100,000 CFU(colony formed units)/mL Entercoccus species isolated,

ID (identify), and MIC (minimum inhibatory concentration) to follow
UA culture grows as follow: >or= than three differnt colonies on the TSA (Tryptic Sol Agar), MAC-NG (MaConkey agar plate - no growth), Enterococcal-100 colonies report as:
greater than three organisms isolated, contaminated specimen. Please recollect.
UA culture grows as follow: TSA-NG, MAC-NG, Enterococcal-NG report as:
no growth at 24 hours
TSA > 100 colonies (same), MAC > 100 colonies (same), Enterococcal-NG report as:
cc >or= 100,000 CFU (colony formed units)/mL gram-negative bacilli isolated

ID (identify) and MIC (minimum inhibatory concentration) to follow
List the three flagellates infections to humans?
1) Giardia lambia
2) Trichomonas vaginalis
3) Trypanosoma species
How do humans get flagellates infections?
1) cysts contaminated in food or water. Fecal oral route.
2) STD
3) arthropod (insect) bite
Amebic dysentery is caused by which significant pathogen?
Entamoeba histolyica
In which stage of an Entamoeba histolytica mutiplies in the intestine?
Trophozites
Which parasite is commonly identified by the anal impression-smear or cellophane tape method?
Enterobius vermicularis (pinworm)
A person becomes infected with tapeworms by eating the larvae infected in?
poorly cooked beef or pork
The permanent smear made for identifying parasites covered in lab is known as?
trichrome method (stain)
Chagas disease is caused by which parasite?
trypanosoma cruzi
List the characteristics and syndrome cause by Balantidium coli.
Ciliate protozoa, that causes dysentery-like syndrome
List the characteristics of tapeworms.
Ribbon like flatworms composted of individual segments (proglottids) each which contains both male and female sex organs. The scolex has hooklets and suckers.
List the basic structure of all protozoa.
The nucleus is well defined by a nuclear membrane, lying with cytoplasm, enclosed by a thin outer membrane
In malaria, the parasite has two life cycles, which one occurs in humans?
asexual in humans, sexual in mosquitoes
One group of round worms carried by arthtopods (insects) and enter the body by way of an insect bite is:
the filaria
What other organism is also gram-negative diplococci?
Moraxella catarrhalis
Syphilis is also know as?
Treponema pallidum
What organism produces mucoid colonies?
streptococcus pneumoniae
What organism produces an enzyme called pyrrolldonylaryl amidase?
Entercocci Group D species
What is the gram stain of Campylobacter spp.?
gram-negative curved rods
Campy plates are placed in what kinds of conditions?
Microaerophlic
Tapeworm in beef
taenia saginata
Tapeworm in pork
taenia solium
Coagulase Negative Staphlococcus (CNS)

catalase:__________
coagulase:__________
Coagulase Negative Staphlococcus (CNS)

catalase: postive (+)
coagulase: negative (-)
What organism(s) produce a swarming apperance on TSA + 5% SB?
p. mirabilis
p. vulgaris
Urinary Tract infections in young women?
Staphlococcus saprophyticus
Staphlococcus spp. are __________ to TAXO-A (Bacitracin)
resistant
An adultworm is usually considered under what species?
Taenia spp.
Another name for tapeworms is...
Intestinal cestodes