Final Review For Micro Lab

Front 1

transformation

Back 1

uptake of DNA by competent cell

Front 2

Acinetobacter spp. ADP-1

Back 2

wild-type, can grow on POB

Front 3

POB

Back 3

p- hydroxyhenzoate

Front 4

Acinetobacter spp. ADP-6

Back 4

mutant, lacks enzyme protocatechuate oxygenase

Front 5

TENS solution

Back 5

Tris buffer- pH buffer suppress DNA
EDTA- chelating agent, removes cations
NaOH- breaks cell wall
SDS- detergent to solubilize cell membrane

Front 6

pilius

Back 6

cell-cell contact

Front 7

plasmid

Back 7

closed-circular, extrachromosomal DNA capable of self-replication

Front 8

TOL plasmid

Back 8

conjugable plasmid > breakdown of M-Toluate

Front 9

What are the two ways of DNA transfer?

Back 9

Chromosomal and plasmid transfer

Front 10

Pseudomonas putida PAW-15

Back 10

donor strain, leucine auxotroph> leu-, tol +

Front 11

Pseudomonas putida 503CA

Back 11

receptor strain, prototroph> Leu +, Tol -

Front 12

M-tol

Back 12

none should grow

Front 13

M-tol+ Leucine

Back 13

PAW-15

Front 14

GMM

Back 14

503CA

Front 15

coliphages

Back 15

sewage filtrate

Front 16

viruses

Back 16

noncellular, obligate intracellular parasites

Front 17

bacteria

Back 17

intracellular, plasma membrane, ATP metabolism, ribosomes, senistive to antibotics

Front 18

growing phages

Back 18

visualization of infection using solid media zone of clearing

Front 19

bateriophage titer

Back 19

plaque-forming units

Front 20

algae

Back 20

eukaryotic photoautotrophs, little tissue differentation, no vascular system

Front 21

phycology

Back 21

study of algae

Front 22

importantance of algae

Back 22

agar, food thickener, cosmetics, petroleum

Front 23

green algae

Back 23

terrestrial plants precursor Chlamydomonas (flagellate) Volvox (colonial) Spirogyra (filamentous) Desminds

Front 24

brown algae

Back 24

kelp, seaweed, rapid growth, use as thickener, tires

Front 25

red algae

Back 25

mostly marine, agar source

Front 26

diatoms

Back 26

complex cell walls "diatomaceous earth" - silica cell walls

Front 27

dinoflagellates

Back 27

plankton, red tides, neurotoxin

Front 28

cyanobacteria

Back 28

oxygenic photosynthetic (in thylakoids),blue green bacteria, aquatic, some fix nitrogen in heterocysts, morphology: unicellular, colonial, filamentous

Front 29

protozoa

Back 29

unicellular eukaryote, non-photosynethetic, chemohetertrophs ( except euglenoids that can be photoautotrophs) covered by unit membrane (pellicle)

Front 30

What are the two stages of protozoa?

Back 30

cysts- survival unfavorable conditions
trophozoites- feeding, reproduction

Front 31

importance of protozoa

Back 31

-important in recycling
-natural insecticides
-symbiotic association with animals (digestion in cow's rumen)
-cause human diseases
Chagas disease, Entamoeba histolytica

Front 32

Plasomodium spp.(protozoa)

Back 32

causes moust devasting form of malaria

Front 33

naturally competent cells

Back 33

Bacillius, S.pneumoniae, Acinetobacter spp.

Front 34

artifically competent cells

Back 34

E.coli very important in biotechnology

Front 35

discovery of transformation

Back 35

Griffith 1928

Front 36

Protocatechuate oxygenase

Back 36

required enzyme to breakdown POB

Front 37

GMM

Back 37

glucose minimal medium control growth

Front 38

conjugation

Back 38

unidirectional transfer of genetic material

Front 39

horizontal DNA transfer

Back 39

DNA transfer with similar species

Front 40

GMM grows?

Back 40

503CA, E. coli, no PAW-15

Front 41

M-tol + leucine grows?

Back 41

PAW- 15 (req leucine), 503CA & E. coli cannot break down m-tol

Front 42

M-toluate grows?

Back 42

none grow

Front 43

M-toluate test plate grows

Back 43

PAW-15 transferred conjugated plasmid to 503CA

Front 44

viruses

Back 44

noncellular, obligate intracellular parasites DNA or RNA (capsid)

Front 45

host range

Back 45

receptors: Bacteriophages- phages, Coliphages- E.coli

Front 46

bacteriophage infection pathways

Back 46

lysis- death of host cell
lysogeny- prophage