E. Coli Bacteria Transformation

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Introduction Bacteria is a small prokaryotic that is a single celled organism. There are many kinds of bacteria that are used daily in the medical or research field. Escherichia coli is a bacteria that is found in the intestines that is not harmful and is used by many laboratories where they perform many experiments with it. Researchers value Escherichia coli for its genetic simplicity (it has about 4400 genes, versus the 30,000 in a human cell) and for its fast growth (Feltman). One of the research that is very popular with Escherichia coli is bacteria transformation.
Transformation, in biology, one of several processes by which genetic material in the form of “naked” deoxyribonucleic acid (DNA) is transferred between microbial cells ("Transformation | Biology"). In this experiment the main focus is to demonstrate and explore how to transform bacteria with new DNA so that is expresses different genetic information. This is done by affecting the bacteria transformation of E coli bacteria by using heat shock and incubation. The hypothesis for this study is that the one with no antibiotic or dilution there would be tremendous amount of E. Coli bacteria and with antibiotic (ampicillin) the amount of E. Coli will be lower however if the E. Coli is diluted the amount of bacteria count will be lower because the E. Coli is resistant to ampicillin.
Methods …show more content…
First step is to determine the total mass of plasmid DNA used. Then, calculate the total volume of cell suspension prepared. Next, calculate the fraction of the total cell suspension that was spread on the plate. Next, determine the mass of plasmid DNA in cell suspension (total mass of plasmid DNA x fraction spread). Final and fifth step is to determine the number of colonies per μg of plasmid DNA (number of colonies observed/step 4) .Below are the example calculations for both T10 and T10d to demonstrate the difference in

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