ESKAPE pathogens are six organisms that bring a huge threat to human, because they are drug-resistance. Scientists have not found antibiotics that are effective since the pathogens are multi-drug resistance. Multi-drug resistance is top three on global public health that are mostly caused by nosocomial infections (hospital-acquired infection caused by bacteria, fungal and viral, parasites and other pathogens). The six pathogens are Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumonia, Acinetobacter baumannii, Pseudomonas aeruginosa, and several species of Enterobacter. Instead of using these ESKAPE pathogens in classroom laboratories that are dangerous to human, we use their safe relatives that does not bring a huge risk to the human life. Safe relatives have almost the same anatomy and physiology features to their ESKAPE counterparts without causing harm to the human. The safe
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The purpose of this experiment was to make copies of DNA. Each student has tube containing Taq polymerase, dNTP’s, polymerase buffer, 27F primer, 1492R primer and di water. We mixed isolated bacteria by using a toothpick into 5 μL di of sterile water. Add 1 μL of the mixed colony with DI water into the tube. While performing this experiment we did a gram stain of the same colony. The last thing we did was gel electrophoresis. The purpose of this experiment was to separate DNA, according to its size. For this part of an experiment, we mixed 5 μL of sample with 1 μL of iodine so that the DN A will not float. Instead it will stay inside the well. Then transfer 5 μL of the mixture to designated well. Turn on the gel electrophoresis for 10 mins until it finishes running. After it finishes running send the results to sequencing company. At last take the result from sequencing company and Nucleotide BLAST it to get the genus and the species of the
The purpose of this experiment was to make copies of DNA. Each student has tube containing Taq polymerase, dNTP’s, polymerase buffer, 27F primer, 1492R primer and di water. We mixed isolated bacteria by using a toothpick into 5 μL di of sterile water. Add 1 μL of the mixed colony with DI water into the tube. While performing this experiment we did a gram stain of the same colony. The last thing we did was gel electrophoresis. The purpose of this experiment was to separate DNA, according to its size. For this part of an experiment, we mixed 5 μL of sample with 1 μL of iodine so that the DN A will not float. Instead it will stay inside the well. Then transfer 5 μL of the mixture to designated well. Turn on the gel electrophoresis for 10 mins until it finishes running. After it finishes running send the results to sequencing company. At last take the result from sequencing company and Nucleotide BLAST it to get the genus and the species of the