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43 Cards in this Set

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COMPOUND LIGHT MICROSCOPE
AN INSTRUMENT WITH TWO SETS OF LENSES THAT USES VISIBLE LIGHT AS A SOURCE OF ILLUMINATION
MICROMETER
A UNIT OF MEASUREMENT EQUAL TO 1026m
FLUORESCENCE
THE ABILITY OF A SUBSTANCE TO GIVE OFF LIGHT OF ONE COLOR WHEN EXPOSED TO THE LIGHT OF ANOTHER COLOR
FLAGELLUM (pl. FLAGELLA)
A THIN APPENDAGE FROM THE SURFACE OF A CELL; USED FOR CELLULAR LOCOMOTION; COMPOSED OF FLAGELLIN IN PROKARYOTIC CELLS, COMPOSED OF NINE PAIRS PLUS TWO MICROTUBULES IN EUKARYOTIC CELLS
CONDENSER
A LENSE SYSTEM LOCATED BELOW THE MICROSCOPE STAGE THAT DIRECTS LIGHT RAYS THROUGH THE SPECIMEN
ENDOSPORE
A RESTING STRUCTURE FORMED INSIDE SOME BACTERIA
OBJECTIVE LENSES
IN A COMPOUND LIGHT MICROSCOPE, THE LENSES CLOSEST TO THE SPECIMEN
SCANNING ELECTRON MICROSCOPE (SEM)
AN ELECTRON MICROSCOPE THAT PROVIDES THREE-DIMENSIONAL VIEWS OF THE SPECIMEN MAGNIFIED 1,000 - 10,000x
COUNTERSTAIN
A SECOND STAIN APPLIED TO A SMEAR, PROVIDES CONTRAST TO THE PRIMARY STAIN
GRAM-POSITIVE BACTERIA
BACTERIA THAT RETAIN THE CRYSTAL VIOLET COLOR, AFTER DECOLORIZING WITH ALCOHOL; THEY STAIN DARK PURPLE
RESOLUTION
THE ABILITY TO DISTINGUISH FINE DETAIL WITH A MAGNIFYING INSTRUMENT; ALSO CALLED RESOLVING POWER
ACIDIC DYE
A SALT IN WHICH THE COLOR IS IN THE NEGATIVE ION; USED FOR NEGATIVE STAINING
TRANSMISSION ELECTRON MICROSCOPE (TEM)
AN ELECTRON MICROSCOPE THAT PROVIDES HIGH MAGNIFICATIONS(10,000-100,000x)OF THIN SECTIONS OF A SPECIMEN
FIXING
(1) IN SLIDE PREPARATION, THE PROCESS OF ATTACHING A SPECIMEN TO A SLIDE (2)REGARDING CHEMICAL ELEMENTS, COMBINING ELEMENTS SO THAT A CRITICAL ELEMENT CAN ENTER THE FOOD CHAIN
NANOMETER (nm)
A MEASUREMENT EQUAL TO 1029m, 1023mm
BRIGHTFIELD MICROSCOPE
A MICROSCOPE THAT USES VISIBLE LIGHT FOR ILLUMINATION; THE SPECIMENS ARE VIEWED AGAINST A WHITE BACKGROUND
FLUORESCENCE MICROSCOPE
A MICROSCOPE THAT USES AN ULTRAVIOLET LIGHT SOURCE TO ILLUMINATE SPECIMENS THAT WILL FLUORESCE
PHASE-CONTRAST MICROSCOPE
A COMPOUND LIGHT MICROSCOPE THAT ALLOWS EXAMINATION OF STRUCTURES INSIDE CELLS THROUGH THE USE OF A SPECIAL CONDENSER
BASIC DYE
A SALT THAT THE COLOR IS IN THE POSITIVE ION; USED FOR BACTERIAL STAINS
NEGATIVE STAINING
A PROCEDURE THAT RESULTS IN COLORLESS BACTERIA AGAINST A STAINED BACKGROUND
DIFFERENTIAL INTERFERENCE CONTRAST (DIC) MICROSCOPE
AN INSTRUMENT THAT PROVIDES A THREE-DIMENSIONAL, MAGNIFIED IMAGE
ELECTRON MICROSCOPE
A MICROSCOPE THAT USES ELECTRONS INSTEAD OF LIGHT TO PRODUCE AN IMAGE
GRAM-POSITIVE CELL WALL
THE CELL WALL OF MOST GRAM-POSITIVE BACTERIA, CONSISTING OF PEPTIDOGLYCAN AND TEICHOIC ACIDS
CONFOCAL MICROSCOPY
A LIGHT MICROSCOPE THAT USES FLUORESCENT STAINS AND LASER TO MAKE TWO- AND THREE-DIMENSIONAL IMAGES
SCANNED PROBE MICROSCOPY
MICROSCOPIC TECHNIQUE USED TO OBTAIN IMAGES OF MOLECULAR SHAPES, TO CHARACTERIZE CHEMICAL PROPERTIES, AND TO DETERMINE TEMPERATURE VARIATIONS WITHIN A SPECIMEN
OCULAR LENSE
IN A COMPOUND LIGHT MICROSCOPE, THE LENSE CLOSEST TO THE VIEWER; ALSO CALLED THE EYEPIECE
REFRACTIVE INDEX
THE RELATIVE VELOCITY WITH WHICH LIGHT PASSES THROUGH A SUBSTANCE
FLUORESCENT-ANTIBODY (FA) TECHNIQUE
A DIAGNOSTIC TOOL USING ANTIBODIES LABELED WITH FLUOROCHROMES AND VIEWED THROUGH A FLUORESCENCE MICROSCOPE; ALSO CALLED IMMUNOFLUORESCENCE
SIMPLE STAIN
A METHOD OF STAINING MICROORGANISMS WITH A SINGLE BASIC DYE
CAPSULE
AN OUTER, VISCOUS COVERING ON SOME BACTERIA, COMPOSED OF A POLYSACCHARIDE OR POLYPEPTIDE
TOTAL MAGNIFICATION
THE MAGNIFICATION OF A MICROSCOPIC SPECIMEN, DETERMINED BY MULTIPLYING THE OCULAR LENS MAGNIFICATION BY THE OBJECTIVE LENS MAGNIFICATION
MORDANT
A SUBSTANCE ADDED TO A STAINING SOLUTION TO MAKE IT STAIN MORE INTENSELY
DARKFIELD MICROSCOPE
A MICROSCOPE THAT HAS A DEVICE TO SCATTER LIGHT FROM THE ILLUMINATOR SO THAT THE SPECIMEN APPEARS WHITE AGAINST A BLACK BACKGROUND
DIFFERENTIAL STAIN
A STAIN THAT DISTINGUISHES OBJECTS ON THE BASIS OF REACTIONS TO THE STAINING PROCEDURE
ACID-FAST STAIN
A DIFFERENTIAL STAIN USED TO IDENTIFY BACTERIA THAT ARE NOT DECOLORIZED BY ACID-ALCOHOL
GRAM STAIN
A DIFFERENTIAL STAIN THAT CLASSIFIES BACTERIA INTO TWO GROUPS, GRAM-NEGATIVE AND GRAM-POSITIVE
GRAM-NEGATIVE BACTERIA
BACTERIA THAT LOSE THE CRYSTAL VIOLET COLOR AFTER DECOLORIZATION BY ALCOHOL; THEY STAIN RED AFTER TREATMENT WITH SAFRANIN
STAINING
COLORIZING WITH A DYE TO VIEW THROUGH A MICROSCOPE OR TO VISUALIZE SPECIFIC STRUCTURES
DECOLORIZING AGENT
A SOLUTION USED IN THE PROCESS OF REMOVING A STAIN
ANTIBODY
A PROTEIN PRODUCED BY THE BODY IN RESPONSE TO AN ANTIGEN, AND CAPABLE OF COMBINING SPECIFICALLY WITH THAT ANTIGEN
GRAM-NEGATIVE CELL WALL
THE CELL WALL OF A GRAM-NEGATIVE BACTERIA, A PEPTIDOGLYCAN LAYER SURROUNDED BY A LIPOPOLYSACCHARIDE OUTER MEMBRANE
SMEAR
A THIN FILM OF MATERIAL CONTAINING MICROORGANISMS, SPREAD OVER THE SURFACE OF A SLIDE
VIRULENCE
THE DEGREE OF PATHOGENICITY OF A MICROORGANISM