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3 Cards in this Set

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  • Back

Gram stain procedure

1) Crystal violet x1 min --> wash with water


2) Iodine x1 min --> wash with water


3) Acetone-alcohol wash --> wash with water


4) Safranin --> wash with water

Purpose of iodine in gram stain

Serves as a mordant to bind the crystal violet

Acridine orange stain:


- Purpose


- Type of stain

- To highlight low numbers of bacteria or bacteria in a dense background of debris


- Fluorescent