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Gram stain procedure
1) Crystal violet x1 min --> wash with water
2) Iodine x1 min --> wash with water
3) Acetone-alcohol wash --> wash with water
4) Safranin --> wash with water
Purpose of iodine in gram stain
Serves as a mordant to bind the crystal violet
Acridine orange stain:
- Purpose
- Type of stain
- To highlight low numbers of bacteria or bacteria in a dense background of debris
- Fluorescent
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