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11 Cards in this Set

  • Front
  • Back

Citrate utilization

Green agar slant


1 loop stab to the bottom of tube then streak slant

Starch Hydrolysis

Starch plate


1 loopful


Streak a single line on plate

Phenol Red sugar fermentation

Red media with Durham tube


Add one loopful to broth

Nitrate Reduction

Nitrate Broth


Add 1 loopful to broth

Methyl Red

Pale yellow broth


Add 1 loopful to broth

How to get results of Citrate Utilization test.

Look at tube


Deep blue means positive


Green mean negative

How to get results of starch hydrolysis test

Add grams iodine to plate put over a white piece of paper


If positive it will have a halo around it


If negative no halo

How to get results of phenol red test

Look for color change to yellow and if there is gas in Durham tube


If positive for acid color will be yellow


If positive for gas the Durham tube will be filled with gas


If negative there will be no color change or gas in Durham tube

How to get results of nitrate reduction test.

3 drops of nitrate A and 3 drops of nitrate B let sit for 15 minutes periodically swirling


If positive color change will be red


If negative color will be pale yellow

How to get results of vp test

Pipette 1ml from mrvp tube into a new empty tube


Add 18 drops of barrettes A & B


If positive color will be Deep red


If negative color will be brown

How to get results of mr test

Add 3 drops of methyl red


If positive color will be red


If negative color will not change