Med Micro Lab Practical

Front 1

a medium that contains living microbes

Back 1

culture

Front 2

a culture that contains a single species

Back 2

pure culture

Front 3

without contamination of yourself, others, the environment, the source culture, or the medium being inoculated

Back 3

aseptic

Front 4

what are the seven general techniques that improve your chances of making an aseptic transfer?

Back 4

1.) minimize the potential of contamination

2.) be organized

3.) place all media tubes in a test tube rack when not in use whether they are sterile or not

4.) take your time

5.) never hold a tube culture by it's cap

6.) hold the inoculating loop or needle like a pencil in your dominant hand and relax

7.) adjust your bunsen burner so its flame has an inner and outer cone

Front 5

where should you write on your petri plates?

Back 5

the base

Front 6

used to grow microbes when fresh cultures or large numbers of cells are required

Back 6

broths

Front 7

generally used to grow stock cultures that can be refrigerated after incubation and maintained for several weeks

Back 7

agar slants

Front 8

typically used for obtaining isolation of species, differential testing, and quantifying bacterial densities

Back 8

plated media

Front 9

instruments usually used for transfers

Back 9

inoculating loops or needles

Front 10

what is the concern with BSL-2 organisms?

Back 10

aerosol production

Front 11

what do you label with?

Back 11

your name, the date, the medium, and the organism's name

Front 12

what are two important things to remember about aseptic technique? (according to class)

Back 12

1.) exclusion

2.) heat

Front 13

group of microorganisms that are visible to the naked eye

Back 13

colony

Front 14

what are the 6 aspects of colony morphology?

Back 14

1.) color

2.) shape

3.) size

4.) texture

5.) elevation

6.) margin-edge of colony

Front 15

a microbial culture consisting of two or more species

Back 15

mixed culture

Front 16

the colony origin

Back 16

colony-forming unit (CFU)

Front 17

way to separate bacteria

Back 17

streak plate

Front 18

functions of a streak plate?

Back 18

1.) separate bacteria to form isolated colonies (pure culture)

2.) check purity (colony morphology)