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6 Cards in this Set
- Front
- Back
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What terminates DNA synthesis? |
The addition of a ddNTP |
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Why do ddNTPs terminate synthesis? |
They have a 3' H instead of a 3' OH |
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Ingredients for Sanger Sequencing? |
4 ddNTPs DNA Template Primer DNA Polymerase |
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Where does the radioactive label attach to? |
The 5' end |
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Ratio of ddNTPs to dNTPs? |
1 ddNTP/50 dNTP |
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How are the fragments separated/visualized? |
Separated or denatured by polyacrylamide gel and detected by flourescent dyes or autoradiography |
