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13 Cards in this Set
- Front
- Back
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DNA synthesis |
dNTPs are attached to 3’OH 2 phosphates release |
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Sanger sequencing |
Back (Definition) |
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DNA pol for sequencing needs: |
High processivity No exonuclease activity Thermostable Taq normally used
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Contrasting with PCR |
PCR- exponential increase in produce- forward and reverse strand together Sanger- linear increase- forward and reverse strands sequences separately |
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Contrasting with PCR |
PCR- exponential increase in produce- forward and reverse strand together Sanger- linear increase- forward and reverse strands sequences separately |
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Uses of Sanger in real life |
Back (Definition) |
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Contrasting with PCR |
PCR- exponential increase in produce- forward and reverse strand together Sanger- linear increase- forward and reverse strands sequences separately |
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|
Uses of Sanger in real life |
Back (Definition) |
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Next generation sequencing |
Back (Definition) |
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Contrasting with PCR |
PCR- exponential increase in produce- forward and reverse strand together Sanger- linear increase- forward and reverse strands sequences separately |
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|
Uses of Sanger in real life |
Back (Definition) |
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Next generation sequencing |
Back (Definition) |
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Sequence by synthesis |
Back (Definition) |
