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28 Cards in this Set
- Front
- Back
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Trypsin can cleave: |
R, K |
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Chymotrypsin can cleave: |
F, Y, W |
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Clostripain can cleave: |
R |
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Staphylococcal Protease can cleave: |
D, E |
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Elastase can cleave: |
A |
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Thermolysin can cleave: |
I, L, V (at N terminal) |
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Cyanogen Vromide can cleave: |
M |
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Hydroxylamine can cleave: |
N-G bond |
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What reagents can be used for determining N terminal? (3) |
Phenylisothiocyanate, 2,4-dinitrofluorobenzene and Dansyl Chloride |
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Carboxypeptidase A can cleave: |
All except P, R, K |
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Carboxypeptidase B can cleave |
Only R and K |
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Carboxypeptidase C can cleave: |
Any residue |
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Carboxypeptidase Y can cleave: |
Any residue |
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SDS PAGE stands for |
Sodium Dodecyl Sulfate- Polyacrylamide Gel Electrophoresis |
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SDS-PAGE separates proteins by: |
Size |
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SDS-PAGE can be used to determine a protein's ________ |
Molecular Weight |
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A type of electrophoresis that uses protein's PI to separate |
Isoelectric focusing |
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When Isoelectric focusing and SDS PAGE is combined |
2D gel electrophoresis |
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Use of electric current to move charged particles |
Electrophoresis |
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Dowex-50 is used as what? |
Cation exchanger |
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Dowex-1 is used as______ |
Anion exchanger |
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Carboxymethyl Cellulose is used as _____ |
Cation exchanger |
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Chelex-100 is used as ______ |
Cation exchanger |
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DEAE (diethyl aminoethyl cellulose is used as ______ |
Anion exchanger |
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A type of chromatography that uses charge to retard analyte |
Ion exchange chromatography |
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In size exclusion chromatography ________ elutes first |
Large molecules |
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Bio-gel and dextran/argose can be used as stationary phase for what type of chromatography? |
Size exclusion |
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At 15,000×g centrifugal spin what will be the precipitate? |
Mitochondria |
