Pacific Biosciences is the manufacturer that develops comprehensive solution for the used if scientist that stimulate the field of genomics. They also stimulate the field of genomics, improve in science and its research and create positive impact through globally. PacBio also contribute in resolving complex genetic challenges by revolutionized the methods. PacBio tried to develop a new novel technology that is able to push the boundaries of the sequencing in order to overcome the challenges in the field of genomics. PacBio RS II is a Single Molecule Real-Time (SMRT) DNA Sequencing System that gives the highest accuracy and longest read length of the available sequencing technology. PacBio RS II was commercially released in April 2013. PacBio
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In this new technology, there are two approaches involve which are phospholinked nucleotides and Zero Waveguide (ZMW). The sequencing begins with a single DNA polymerase is immobilized at the bottom of a reaction cell and the reaction called a ZMW (Zero Mode Waveguide). DNA is replicated by enzymes called DNA polymerase which is efficiently duplicate the entire genomes in minutes by reading the DNA and sequentially building a complementary with matching building blocks called nucleotides. DNA polymerase bound to the DNA template is anchored to the bottom glass surface of ZMW. In this sequencing, a phospholinked dNTP is used and each dNTP contains a different fluorophore. During sequence, a single labelled dNTP enters the polymerase. In order to visualize polymerase activity, a different coloured fluorescent label is attached to the four nucleotides, A, C, G and T. Fast bowling nucleotides carry their fluorescent label on the terminal phosphate rather than the base. Indeed, through this innovation, the enzyme cleaves away the fluorescent label as part of the incorporation process.
In detection and sequence determination, the fluorescence signals for each ZMW are collected. Data is collected as a movie of the sequential signals and each individual signal is measured as a short pulse of light. Laser light travelling through the glass into the ZMW illuminates only the lower 30 nm of it since the ZMW dimensions are smaller than the wavelength of the light. This allows the selective excitation and identification of light emitted from nucleotides recruited base elongations that are being held by the polymerase for fractions of
In this new technology, there are two approaches involve which are phospholinked nucleotides and Zero Waveguide (ZMW). The sequencing begins with a single DNA polymerase is immobilized at the bottom of a reaction cell and the reaction called a ZMW (Zero Mode Waveguide). DNA is replicated by enzymes called DNA polymerase which is efficiently duplicate the entire genomes in minutes by reading the DNA and sequentially building a complementary with matching building blocks called nucleotides. DNA polymerase bound to the DNA template is anchored to the bottom glass surface of ZMW. In this sequencing, a phospholinked dNTP is used and each dNTP contains a different fluorophore. During sequence, a single labelled dNTP enters the polymerase. In order to visualize polymerase activity, a different coloured fluorescent label is attached to the four nucleotides, A, C, G and T. Fast bowling nucleotides carry their fluorescent label on the terminal phosphate rather than the base. Indeed, through this innovation, the enzyme cleaves away the fluorescent label as part of the incorporation process.
In detection and sequence determination, the fluorescence signals for each ZMW are collected. Data is collected as a movie of the sequential signals and each individual signal is measured as a short pulse of light. Laser light travelling through the glass into the ZMW illuminates only the lower 30 nm of it since the ZMW dimensions are smaller than the wavelength of the light. This allows the selective excitation and identification of light emitted from nucleotides recruited base elongations that are being held by the polymerase for fractions of