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706 Cards in this Set
- Front
- Back
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What dye is used for a simple staying? |
Methylene blue |
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How long do you let a simple stain sit? |
One minute. |
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What is typically used to inoculate slant tubes? |
Inoculating loop |
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Why are plates put bottoms up? |
To prevent condensation and |
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|
What is an inanimate object that can harbor micro organisms |
Fomite |
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What power is the oil immersion |
100 X |
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What percent auger is TSA auger? |
1.5% |
|
|
What is a Gram stain used for? |
To differentiate between gram-positive and gram-negative bacteria |
|
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What is it called when you put two or more organisms on the same slide? |
Mixed culture smear |
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What is the mordant in a Gram stain?grams iodine |
Grams iodine |
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What is the primary stain used in a Gram stain? |
Crystal violet |
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What is the secondary stain in a Gram Stain stain? |
safranin |
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What is use for decolorization in a Gram stain? |
95% alcohol for 10 to 20 seconds |
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What type of stain is a Gram stain? |
Differential |
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What are the differences which are shown by a Gram stain? |
Differences in cell wall structure |
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Why does a gram-positive bacteria stained purple? |
Because of its thick peptidoglycan layer |
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What color does a gram-negative bacteria stain? |
Red |
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What does the cell wall of gram-negative bacteria consist of? |
Outer membrane of lipoprotein and lipopolysaccharide, and a thin peptidoglycan layer |
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What plating method is used to isolate your cultures? |
Streak-plate method |
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What color of colony will E. coli give you on a TSA plate? |
White |
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What color of colony will Sarratia marcescens form on TSA agar? |
Pink |
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Who developed the Gram stain? |
Hans Christian Graham |
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How old should your cultures be? |
12-24 hours |
|
|
What inoculation method is used for street plating? |
Quadrant streak method |
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|
What kind of auger is TSA? |
General purpose auger. 1.5% |
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What is the red substance produced by S. marcescens called? |
Prodigiosin |
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What is the purpose of streak plate method? |
To isolate individual colonies and species. |
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What is the significance of gram staining? |
It is a fast inexpensive way to identify cell wall structure. It may suggest effective treatment of disease. |
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What are the limitations of gram staining? |
The staining and destaining process is sensitive; results do not necessarily represent phylogenic relationship. |
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What type of media is used to determine motility? |
S I M tube |
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What does S I M stand for? |
Sulfide, Indole, and motility |
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|
Which organisms were used in our motility lab? |
E. coli and klebsiella pneumonia |
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Which organism was motile? |
E. coli |
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|
How far down the tube should and inoculating needle be placed? |
Two thirds of the way down |
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What did you do to the lids on the SIM tube to check for motility? |
Quarter turn loose |
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What species was used to demonstrate swarming motility? |
Proteus Mirabilis |
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What is the visual characteristic that demonstrate swarming motility? |
Swarming waves coming from the initial inoculation. |
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|
What type of milk would you use for demonstration of motility by E. coli? |
Wet mount. |
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What type of flagella does Proteus vulgaris have? |
Peritricious (All over) |
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What is the name of the stain you used for acid-fast staining. |
Kinyoun |
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Is mycobacterium and gram-positive or gram-negative? |
Gram-positive |
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What is the purpose for acid-fast staining? |
To check for the presence of my colic acid in the cell wall. |
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|
What characteristic of mycobacterium prevents it from getting a good Gram stain? |
The presence of my colic acid. |
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What did mycobacterium require in order to get a good stain? |
Long exposure to carbolfuchsin or the application of heat. |
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|
Why is mycobacterium smegmatis difficult to suspend in a drop of water? |
Because it has a waxy consistency. |
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|
What is used as the decolorant in an acid fest staining process |
3% acid alcohol |
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What is the counterstain in an acid-fast stain? |
Methylene blue. |
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|
What color wheel and acid-fast bacteria be? |
Read rods |
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|
What is the resting stage for bacillus and clostridium species? |
Endospores |
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|
What happens to an in the sport during favorable growth conditions? |
It converts into a vegetative cell. |
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|
When our endospores produced? |
During adverse environmental conditions such as the depletion of nutrients, UV exposure, and dehydration. |
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How many times do you flame The smear slide for endospore stain |
10 times |
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Why do you flame the slide 10 times, as opposed to five times and other smears? |
Because endospores are difficult to kill. |
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|
What die is used for Stadie end of spores? |
Brilliant green, sit for five minutes. |
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What is the secondary stain in endospore staining, and what color is it, and what will it show. |
Safranin, red, vegetative cells. |
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|
What color will endospores stain? |
Green |
|
|
What kind of stain is an acid-fast stain? |
Differential which test for my colic acid in the cell wall |
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|
What is the purpose of an indirect stain |
Determines morphology in the presence of a capsule. |
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|
What is a mold. |
A fungus made up of hyphae which form mycelium that is seen macroscopically as the fungal colony |
|
|
What is a mold. |
A fungus made up of hyphae which form mycelium that is seen macroscopically as the fungal colony |
|
|
What are the two kinds of asexual sports? |
Sporangiospores and conidia |
|
|
What is a mold. |
A fungus made up of hyphae which form mycelium that is seen macroscopically as the fungal colony |
|
|
What are the two kinds of asexual sports? |
Sporangiospores and conidia |
|
|
What are the three kinds of sexual spores? |
Zygospores, Ascospores, basidiospores |
|
|
What is a mold. |
A fungus made up of hyphae which form mycelium that is seen macroscopically as the fungal colony |
|
|
What are the two kinds of asexual sports? |
Sporangiospores and conidia |
|
|
What are the three kinds of sexual spores? |
Zygospores, Ascospores, basidiospores |
|
|
What type of sexual spore does a yeast have? |
Single asexual spore called a blastospore, or a bud |
|
|
What is a mold. |
A fungus made up of hyphae which form mycelium that is seen macroscopically as the fungal colony |
|
|
What are the two kinds of asexual sports? |
Sporangiospores and conidia |
|
|
What are the three kinds of sexual spores? |
Zygospores, Ascospores, basidiospores |
|
|
What type of sexual spore does a yeast have? |
Single asexual spore called a blastospore, or a bud |
|
|
True or false: yeasts have hyphae. |
False. They have multi cellular structures called pseudohyphae. |
|
|
What is a mold. |
A fungus made up of hyphae which form mycelium that is seen macroscopically as the fungal colony |
|
|
What are the two kinds of asexual sports? |
Sporangiospores and conidia |
|
|
What are the three kinds of sexual spores? |
Zygospores, Ascospores, basidiospores |
|
|
What type of sexual spore does a yeast have? |
Single asexual spore called a blastospore, or a bud |
|
|
True or false: yeasts have hyphae. |
False. They have multi cellular structures called pseudohyphae. |
|
|
Which is larger, yeast cells or bacteria cells? |
Yeast |
|
|
What is a mold. |
A fungus made up of hyphae which form mycelium that is seen macroscopically as the fungal colony |
|
|
What are the two kinds of asexual sports? |
Sporangiospores and conidia |
|
|
What are the three kinds of sexual spores? |
Zygospores, Ascospores, basidiospores |
|
|
What type of sexual spore does a yeast have? |
Single asexual spore called a blastospore, or a bud |
|
|
True or false: yeasts have hyphae. |
False. They have multi cellular structures called pseudohyphae. |
|
|
Which is larger, yeast cells or bacteria cells? |
Yeast |
|
|
What type of stain do you use for cell size variation? |
Simple stain with methylene blue |
|
|
What is a mold. |
A fungus made up of hyphae which form mycelium that is seen macroscopically as the fungal colony |
|
|
What are the two kinds of asexual sports? |
Sporangiospores and conidia |
|
|
What are the three kinds of sexual spores? |
Zygospores, Ascospores, basidiospores |
|
|
What type of sexual spore does a yeast have? |
Single asexual spore called a blastospore, or a bud |
|
|
True or false: yeasts have hyphae. |
False. They have multi cellular structures called pseudohyphae. |
|
|
Which is larger, yeast cells or bacteria cells? |
Yeast |
|
|
What type of stain do you use for cell size variation? |
Simple stain with methylene blue |
|
|
What type of stain do you use for microscopic examination of fungi? |
Lactophenol cotton blue |
|
|
What is a mold. |
A fungus made up of hyphae which form mycelium that is seen macroscopically as the fungal colony |
|
|
What are the two kinds of asexual sports? |
Sporangiospores and conidia |
|
|
What are the three kinds of sexual spores? |
Zygospores, Ascospores, basidiospores |
|
|
What type of sexual spore does a yeast have? |
Single asexual spore called a blastospore, or a bud |
|
|
True or false: yeasts have hyphae. |
False. They have multi cellular structures called pseudohyphae. |
|
|
Which is larger, yeast cells or bacteria cells? |
Yeast |
|
|
What type of stain do you use for cell size variation? |
Simple stain with methylene blue |
|
|
What type of stain do you use for microscopic examination of fungi? |
Lactophenol cotton blue |
|
|
What were the names of the fungi observed? |
Aspergillus species, Penicillium species, and Rhizopus species |
|
|
What is a mold. |
A fungus made up of hyphae which form mycelium that is seen macroscopically as the fungal colony |
|
|
What are the two kinds of asexual sports? |
Sporangiospores and conidia |
|
|
What are the three kinds of sexual spores? |
Zygospores, Ascospores, basidiospores |
|
|
What type of sexual spore does a yeast have? |
Single asexual spore called a blastospore, or a bud |
|
|
True or false: yeasts have hyphae. |
False. They have multi cellular structures called pseudohyphae. |
|
|
Which is larger, yeast cells or bacteria cells? |
Yeast |
|
|
What type of stain do you use for cell size variation? |
Simple stain with methylene blue |
|
|
What type of stain do you use for microscopic examination of fungi? |
Lactophenol cotton blue |
|
|
What were the names of the fungi observed? |
Aspergillus species, Penicillium species, and Rhizopus species |
|
|
How many microliters does it inoculating loop contain? |
Approximately 10 |
|
|
What is a mold. |
A fungus made up of hyphae which form mycelium that is seen macroscopically as the fungal colony |
|
|
What constitutes a countable plate? |
30-300 colonies |
|
|
What are the two kinds of asexual sports? |
Sporangiospores and conidia |
|
|
What are the three kinds of sexual spores? |
Zygospores, Ascospores, basidiospores |
|
|
What type of sexual spore does a yeast have? |
Single asexual spore called a blastospore, or a bud |
|
|
True or false: yeasts have hyphae. |
False. They have multi cellular structures called pseudohyphae. |
|
|
Which is larger, yeast cells or bacteria cells? |
Yeast |
|
|
What type of stain do you use for cell size variation? |
Simple stain with methylene blue |
|
|
What type of stain do you use for microscopic examination of fungi? |
Lactophenol cotton blue |
|
|
What were the names of the fungi observed? |
Aspergillus species, Penicillium species, and Rhizopus species |
|
|
How many microliters does it inoculating loop contain? |
Approximately 10 |
|
|
What is a mold. |
A fungus made up of hyphae which form mycelium that is seen macroscopically as the fungal colony |
|
|
What constitutes a countable plate? |
30-300 colonies |
|
|
True or false. In the poor plate method, you have colonies which are embedded into the auger. |
True. |
|
|
What are the two kinds of asexual sports? |
Sporangiospores and conidia |
|
|
What are the three kinds of sexual spores? |
Zygospores, Ascospores, basidiospores |
|
|
What type of sexual spore does a yeast have? |
Single asexual spore called a blastospore, or a bud |
|
|
True or false: yeasts have hyphae. |
False. They have multi cellular structures called pseudohyphae. |
|
|
Which is larger, yeast cells or bacteria cells? |
Yeast |
|
|
What type of stain do you use for cell size variation? |
Simple stain with methylene blue |
|
|
What type of stain do you use for microscopic examination of fungi? |
Lactophenol cotton blue |
|
|
What were the names of the fungi observed? |
Aspergillus species, Penicillium species, and Rhizopus species |
|
|
How many microliters does it inoculating loop contain? |
Approximately 10 |
|
|
What is the name of the apparatus used for mixing test tube broths? |
The vortex |
|
|
Before determining the number of colony forming units per milliliters you must first do what to the sample? |
Dilute the sample through serial dilution |
|
|
Before determining the number of colony forming units per milliliters you must first do what to the sample? |
Dilute the sample through serial dilution |
|
|
What method is used for evenly dispersing bacteria on top of Argus medium? It's just clears |
Spread plate method. |
|
|
Before determining the number of colony forming units per milliliters you must first do what to the sample? |
Dilute the sample through serial dilution |
|
|
What method is used for evenly dispersing bacteria on top of Argus medium? It's just clears |
Spread plate method. |
|
|
What tool is used to spread the culture onto the media surface? |
Hockey stick. |
|
|
Before determining the number of colony forming units per milliliters you must first do what to the sample? |
Dilute the sample through serial dilution |
|
|
What method is used for evenly dispersing bacteria on top of Argus medium? It's just clears |
Spread plate method. |
|
|
What tool is used to spread the culture onto the media surface? |
Hockey stick. |
|
|
What tool was used for precise dilution from one tube to another? |
Micropipette |
|
|
Before determining the number of colony forming units per milliliters you must first do what to the sample? |
Dilute the sample through serial dilution |
|
|
What method is used for evenly dispersing bacteria on top of Argus medium? It's just clears |
Spread plate method. |
|
|
What tool is used to spread the culture onto the media surface? |
Hockey stick. |
|
|
What tool was used for precise dilution from one tube to another? |
Micropipette |
|
|
What message involves evenly dispersing bacteria on top of the Argo's medium? |
Spread plate method. |
|
|
Before determining the number of colony forming units per milliliters you must first do what to the sample? |
Dilute the sample through serial dilution |
|
|
What method is used for evenly dispersing bacteria on top of Argus medium? It's just clears |
Spread plate method. |
|
|
What tool is used to spread the culture onto the media surface? |
Hockey stick. |
|
|
What tool was used for precise dilution from one tube to another? |
Micropipette |
|
|
What method involves evenly dispersing bacteria on top of the Argose medium? |
Spread plate method. |
|
|
Before determining the number of colony forming units per milliliters you must first do what to the sample? |
Dilute the sample through serial dilution |
|
|
What method is used for evenly dispersing bacteria on top of Argus medium? It's just clears |
Spread plate method. |
|
|
What tool is used to spread the culture onto the media surface? |
Hockey stick. |
|
|
What tool was used for precise dilution from one tube to another? |
Micropipette |
|
|
What method involves evenly dispersing bacteria on top of the Argose medium? |
Spread plate method. |
|
|
What is the spread plate method used for? |
Precise measurements. It is use greatly in research labs were consistency, reproducibility, and precise serial dilution's are needed for obtaining accurate data |
|
|
Before determining the number of colony forming units per milliliters you must first do what to the sample? |
Dilute the sample through serial dilution |
|
|
What method is used for evenly dispersing bacteria on top of Argus medium? It's just clears |
Spread plate method. |
|
|
What tool is used to spread the culture onto the media surface? |
Hockey stick. |
|
|
What tool was used for precise dilution from one tube to another? |
Micropipette |
|
|
What method involves evenly dispersing bacteria on top of the Argose medium? |
Spread plate method. |
|
|
What is the spread plate method used for? |
Precise measurements. It is use greatly in research labs were consistency, reproducibility, and precise serial dilution's are needed for obtaining accurate data |
|
|
What is agar? |
A complex polysaccharide isolated from seaweed that is the solidify an agent used in media preparation. It is usually added in a concentration of 1.5% |
|
|
Before determining the number of colony forming units per milliliters you must first do what to the sample? |
Dilute the sample through serial dilution |
|
|
What method is used for evenly dispersing bacteria on top of Argus medium? It's just clears |
Spread plate method. |
|
|
What tool is used to spread the culture onto the media surface? |
Hockey stick. |
|
|
What tool was used for precise dilution from one tube to another? |
Micropipette |
|
|
What method involves evenly dispersing bacteria on top of the Argose medium? |
Spread plate method. |
|
|
What is the spread plate method used for? |
Precise measurements. It is use greatly in research labs were consistency, reproducibility, and precise serial dilution's are needed for obtaining accurate data |
|
|
What is agar? |
A complex polysaccharide isolated from seaweed that is the solidify an agent used in media preparation. It is usually added in a concentration of 1.5% |
|
|
What is a differential medium |
A medium that causes bacteria to take on an appearance that distinguishes them from other bacteria. |
|
|
Before determining the number of colony forming units per milliliters you must first do what to the sample? |
Dilute the sample through serial dilution |
|
|
What method is used for evenly dispersing bacteria on top of Argus medium? It's just clears |
Spread plate method. |
|
|
What tool is used to spread the culture onto the media surface? |
Hockey stick. |
|
|
What tool was used for precise dilution from one tube to another? |
Micropipette |
|
|
What method involves evenly dispersing bacteria on top of the Argose medium? |
Spread plate method. |
|
|
What is the spread plate method used for? |
Precise measurements. It is use greatly in research labs were consistency, reproducibility, and precise serial dilution's are needed for obtaining accurate data |
|
|
What is agar? |
A complex polysaccharide isolated from seaweed that is the solidify an agent used in media preparation. It is usually added in a concentration of 1.5% |
|
|
What is a differential medium |
A medium that causes bacteria to take on an appearance that distinguishes them from other bacteria. |
|
|
What are examples of differential mediums? |
Mannitol salt auger, Eosin methylene blue auger. |
|
|
Before determining the number of colony forming units per milliliters you must first do what to the sample? |
Dilute the sample through serial dilution |
|
|
What method is used for evenly dispersing bacteria on top of Argus medium? It's just clears |
Spread plate method. |
|
|
What tool is used to spread the culture onto the media surface? |
Hockey stick. |
|
|
What tool was used for precise dilution from one tube to another? |
Micropipette |
|
|
What method involves evenly dispersing bacteria on top of the Argose medium? |
Spread plate method. |
|
|
What is the spread plate method used for? |
Precise measurements. It is use greatly in research labs were consistency, reproducibility, and precise serial dilution's are needed for obtaining accurate data |
|
|
What is agar? |
A complex polysaccharide isolated from seaweed that is the solidify an agent used in media preparation. It is usually added in a concentration of 1.5% |
|
|
What is a differential medium |
A medium that causes bacteria to take on an appearance that distinguishes them from other bacteria. |
|
|
What are examples of differential mediums? |
Mannitol salt auger, Eosin methylene blue auger. |
|
|
What does mannitol salt auger do? |
Differentiates staphylococcus species based on its ability to ferment mannitol. It results in lowering the pH of the medium, turning the color around the colony from pink to yellow. |
|
|
Before determining the number of colony forming units per milliliters you must first do what to the sample? |
Dilute the sample through serial dilution |
|
|
What is the exception to the staphylococcus reaction on MSA? |
Staphylococcus epidermidis does not ferment mannitol, therefore there is no color change. |
|
|
What method is used for evenly dispersing bacteria on top of Argus medium? It's just clears |
Spread plate method. |
|
|
What tool is used to spread the culture onto the media surface? |
Hockey stick. |
|
|
What tool was used for precise dilution from one tube to another? |
Micropipette |
|
|
What method involves evenly dispersing bacteria on top of the Argose medium? |
Spread plate method. |
|
|
What is the spread plate method used for? |
Precise measurements. It is use greatly in research labs were consistency, reproducibility, and precise serial dilution's are needed for obtaining accurate data |
|
|
What is agar? |
A complex polysaccharide isolated from seaweed that is the solidify an agent used in media preparation. It is usually added in a concentration of 1.5% |
|
|
What is a differential medium |
A medium that causes bacteria to take on an appearance that distinguishes them from other bacteria. |
|
|
What are examples of differential mediums? |
Mannitol salt auger, Eosin methylene blue auger. |
|
|
What does mannitol salt auger do? |
Differentiates staphylococcus species based on its ability to ferment mannitol. It results in lowering the pH of the medium, turning the color around the colony from pink to yellow. |
|
|
Before determining the number of colony forming units per milliliters you must first do what to the sample? |
Dilute the sample through serial dilution |
|
|
What is the exception to the staphylococcus reaction on MSA? |
Staphylococcus epidermidis does not ferment mannitol, therefore there is no color change. |
|
|
What does EMB auger do? |
Differentiates Graham negative rods based on the organisms ability to ferment lactose. |
|
|
What method is used for evenly dispersing bacteria on top of Argus medium? It's just clears |
Spread plate method. |
|
|
What tool is used to spread the culture onto the media surface? |
Hockey stick. |
|
|
What tool was used for precise dilution from one tube to another? |
Micropipette |
|
|
What method involves evenly dispersing bacteria on top of the Argose medium? |
Spread plate method. |
|
|
What is the spread plate method used for? |
Precise measurements. It is use greatly in research labs were consistency, reproducibility, and precise serial dilution's are needed for obtaining accurate data |
|
|
What is agar? |
A complex polysaccharide isolated from seaweed that is the solidify an agent used in media preparation. It is usually added in a concentration of 1.5% |
|
|
What is a differential medium |
A medium that causes bacteria to take on an appearance that distinguishes them from other bacteria. |
|
|
What are examples of differential mediums? |
Mannitol salt auger, Eosin methylene blue auger. |
|
|
What does mannitol salt auger do? |
Differentiates staphylococcus species based on its ability to ferment mannitol. It results in lowering the pH of the medium, turning the color around the colony from pink to yellow. |
|
|
Before determining the number of colony forming units per milliliters you must first do what to the sample? |
Dilute the sample through serial dilution |
|
|
What is the exception to the staphylococcus reaction on MSA? |
Staphylococcus epidermidis does not ferment mannitol, therefore there is no color change. |
|
|
What does EMB auger do? |
Differentiates Graham negative rods based on the organisms ability to ferment lactose. |
|
|
What color does E. coli turn on EMB auger? |
Shiny metallic green |
|
|
What method is used for evenly dispersing bacteria on top of Argus medium? It's just clears |
Spread plate method. |
|
|
What tool is used to spread the culture onto the media surface? |
Hockey stick. |
|
|
What tool was used for precise dilution from one tube to another? |
Micropipette |
|
|
What method involves evenly dispersing bacteria on top of the Argose medium? |
Spread plate method. |
|
|
What is the spread plate method used for? |
Precise measurements. It is use greatly in research labs were consistency, reproducibility, and precise serial dilution's are needed for obtaining accurate data |
|
|
What is agar? |
A complex polysaccharide isolated from seaweed that is the solidify an agent used in media preparation. It is usually added in a concentration of 1.5% |
|
|
What is a differential medium |
A medium that causes bacteria to take on an appearance that distinguishes them from other bacteria. |
|
|
What are examples of differential mediums? |
Mannitol salt auger, Eosin methylene blue auger. |
|
|
What does mannitol salt auger do? |
Differentiates staphylococcus species based on its ability to ferment mannitol. It results in lowering the pH of the medium, turning the color around the colony from pink to yellow. |
|
|
Before determining the number of colony forming units per milliliters you must first do what to the sample? |
Dilute the sample through serial dilution |
|
|
What is the exception to the staphylococcus reaction on MSA? |
Staphylococcus epidermidis does not ferment mannitol, therefore there is no color change. |
|
|
What does EMB auger do? |
Differentiates Graham negative rods based on the organisms ability to ferment lactose. |
|
|
What color does E. coli turn on EMB auger? |
Shiny metallic green |
|
|
What color do lactose fermenters other than E. coli turn on EMB auger? |
Dark colored red or pink |
|
|
What method is used for evenly dispersing bacteria on top of Argus medium? It's just clears |
Spread plate method. |
|
|
What tool is used to spread the culture onto the media surface? |
Hockey stick. |
|
|
What tool was used for precise dilution from one tube to another? |
Micropipette |
|
|
What method involves evenly dispersing bacteria on top of the Argose medium? |
Spread plate method. |
|
|
What is the spread plate method used for? |
Precise measurements. It is use greatly in research labs were consistency, reproducibility, and precise serial dilution's are needed for obtaining accurate data |
|
|
What is agar? |
A complex polysaccharide isolated from seaweed that is the solidify an agent used in media preparation. It is usually added in a concentration of 1.5% |
|
|
What is a differential medium |
A medium that causes bacteria to take on an appearance that distinguishes them from other bacteria. |
|
|
What are examples of differential mediums? |
Mannitol salt auger, Eosin methylene blue auger. |
|
|
What does mannitol salt auger do? |
Differentiates staphylococcus species based on its ability to ferment mannitol. It results in lowering the pH of the medium, turning the color around the colony from pink to yellow. |
|
|
Before determining the number of colony forming units per milliliters you must first do what to the sample? |
Dilute the sample through serial dilution |
|
|
What is the exception to the staphylococcus reaction on MSA? |
Staphylococcus epidermidis does not ferment mannitol, therefore there is no color change. |
|
|
What does EMB auger do? |
Differentiates Graham negative rods based on the organisms ability to ferment lactose. |
|
|
What color does E. coli turn on EMB auger? |
Shiny metallic green |
|
|
What color do lactose fermenters other than E. coli turn on EMB auger? |
Dark colored red or pink |
|
|
What is a selective medium? |
A medium that allows certain bacteria to grow, but is inhibitory of others. |
|
|
What method is used for evenly dispersing bacteria on top of Argus medium? It's just clears |
Spread plate method. |
|
|
What tool is used to spread the culture onto the media surface? |
Hockey stick. |
|
|
What tool was used for precise dilution from one tube to another? |
Micropipette |
|
|
What method involves evenly dispersing bacteria on top of the Argose medium? |
Spread plate method. |
|
|
What is the spread plate method used for? |
Precise measurements. It is use greatly in research labs were consistency, reproducibility, and precise serial dilution's are needed for obtaining accurate data |
|
|
What is agar? |
A complex polysaccharide isolated from seaweed that is the solidify an agent used in media preparation. It is usually added in a concentration of 1.5% |
|
|
What is a differential medium |
A medium that causes bacteria to take on an appearance that distinguishes them from other bacteria. |
|
|
What are examples of differential mediums? |
Mannitol salt auger, Eosin methylene blue auger. |
|
|
What does mannitol salt auger do? |
Differentiates staphylococcus species based on its ability to ferment mannitol. It results in lowering the pH of the medium, turning the color around the colony from pink to yellow. |
|
|
What is MS a media selective for? |
It allows for growth of staphylococcus species, but is inhibitory to Graham negative rods due to its high concentration of salt (7.5%) |
|
|
What is MS a media selective for? |
It allows for growth of staphylococcus species, but is inhibitory to Graham negative rods due to its high concentration of salt (7.5%) |
|
|
What does EMB auger select for? |
Allows for the growth of Graham negative rods, but is inhibitory to gram-positive organisms due to the Eosyn and methylene blue dyes. |
|
|
What is MS a media selective for? |
It allows for growth of staphylococcus species, but is inhibitory to Graham negative rods due to its high concentration of salt (7.5%) |
|
|
What does EMB auger select for? |
Allows for the growth of Graham negative rods, but is inhibitory to gram-positive organisms due to the Eosyn and methylene blue dyes. |
|
|
What is a complex medium? |
Medium in which the exact composition and amounts of the individual amino acid's, vitamins, growth factors and other components that make up the medium are NOT exactly known. |
|
|
What is MS a media selective for? |
It allows for growth of staphylococcus species, but is inhibitory to Graham negative rods due to its high concentration of salt (7.5%) |
|
|
What does EMB auger select for? |
Allows for the growth of Graham negative rods, but is inhibitory to gram-positive organisms due to the Eosyn and methylene blue dyes. |
|
|
What is a complex medium? |
Medium in which the exact composition and amounts of the individual amino acid's, vitamins, growth factors and other components that make up the medium are NOT exactly known. |
|
|
What is a defined medium? |
A medium in which specific chemical composition is known. |
|
|
What is MS a media selective for? |
It allows for growth of staphylococcus species, but is inhibitory to Graham negative rods due to its high concentration of salt (7.5%) |
|
|
What does EMB auger select for? |
Allows for the growth of Graham negative rods, but is inhibitory to gram-positive organisms due to the Eosyn and methylene blue dyes. |
|
|
What is a complex medium? |
Medium in which the exact composition and amounts of the individual amino acid's, vitamins, growth factors and other components that make up the medium are NOT exactly known. |
|
|
What is a defined medium? |
A medium in which specific chemical composition is known. |
|
|
Autoclave |
Machine used to sterilize media. Involves heating at 121°C for 15-20 minutes at 15 psi |
|
|
What is MS a media selective for? |
It allows for growth of staphylococcus species, but is inhibitory to Graham negative rods due to its high concentration of salt (7.5%) |
|
|
What does EMB auger select for? |
Allows for the growth of Graham negative rods, but is inhibitory to gram-positive organisms due to the Eosyn and methylene blue dyes. |
|
|
What is a complex medium? |
Medium in which the exact composition and amounts of the individual amino acid's, vitamins, growth factors and other components that make up the medium are NOT exactly known. |
|
|
What is a defined medium? |
A medium in which specific chemical composition is known. |
|
|
Autoclave |
Machine used to sterilize media. Involves heating at 121°C for 15-20 minutes at 15 psi |
|
|
What is the scientific notation used for the number of colony forming units per milliliter? |
x.y x 106th cfu/ml |
|
|
What is MS a media selective for? |
It allows for growth of staphylococcus species, but is inhibitory to Graham negative rods due to its high concentration of salt (7.5%) |
|
|
What does EMB auger select for? |
Allows for the growth of Graham negative rods, but is inhibitory to gram-positive organisms due to the Eosyn and methylene blue dyes. |
|
|
What is a complex medium? |
Medium in which the exact composition and amounts of the individual amino acid's, vitamins, growth factors and other components that make up the medium are NOT exactly known. |
|
|
What is a defined medium? |
A medium in which specific chemical composition is known. |
|
|
Autoclave |
Machine used to sterilize media. Involves heating at 121°C for 15-20 minutes at 15 psi |
|
|
What is the scientific notation used for the number of colony forming units per milliliter? |
x.y x 106th cfu/ml |
|
|
To determine the tighter, you must know the what? |
Dilution |
|
|
What's the dilution factor is calculated, take the blank and multiply it by the number of colonies counted. |
Reciprocal |
|
|
What's the dilution factor is calculated, take the blank and multiply it by the number of colonies counted. |
Reciprocal |
|
|
What is a graphical representation of the change in population size over time? |
Growth curve |
|
|
What's the dilution factor is calculated, take the blank and multiply it by the number of colonies counted. |
Reciprocal |
|
|
What is a graphical representation of the change in population size over time? |
Growth curve |
|
|
What happens during the lag phase |
Bacteria is adapting to his new |
|
|
What's the dilution factor is calculated, take the blank and multiply it by the number of colonies counted. |
Reciprocal |
|
|
What is a graphical representation of the change in population size over time? |
Growth curve |
|
|
What happens during the lag phase |
Bacteria is adapting to his new |
|
|
What happens during the logarithmic phase? |
Exponential |
|
|
What's the dilution factor is calculated, take the blank and multiply it by the number of colonies counted. |
Reciprocal |
|
|
What is a graphical representation of the change in population size over time? |
Growth curve |
|
|
What happens during the lag phase |
Bacteria is adapting to his new |
|
|
What happens during the logarithmic phase? |
Exponential |
|
|
What happens during the stationary phase |
Reproduction and death dates are equal |
|
|
What's the dilution factor is calculated, take the blank and multiply it by the number of colonies counted. |
Reciprocal |
|
|
What is a graphical representation of the change in population size over time? |
Growth curve |
|
|
What happens during the lag phase |
Bacteria is adapting to his new |
|
|
What happens during the logarithmic phase? |
Exponential |
|
|
What happens during the stationary phase |
Reproduction and death dates are equal |
|
|
What happens during the death phase |
Number of dying sales exceeds the number of newly formed |
|
|
What's the dilution factor is calculated, take the blank and multiply it by the number of colonies counted. |
Reciprocal |
|
|
What is a graphical representation of the change in population size over time? |
Growth curve |
|
|
What happens during the lag phase |
Bacteria is adapting to his new |
|
|
What happens during the logarithmic phase? |
Exponential |
|
|
What happens during the stationary phase |
Reproduction and death dates are equal |
|
|
What happens during the death phase |
Number of dying sales exceeds the number of newly formed |
|
|
To determine a growth curve, samples from actively growing culture are taken over what period of time? |
24-48 hours |
|
|
What's the dilution factor is calculated, take the blank and multiply it by the number of colonies counted. |
Reciprocal |
|
|
What is a graphical representation of the change in population size over time? |
Growth curve |
|
|
What happens during the lag phase |
Bacteria is adapting to his new |
|
|
What happens during the logarithmic phase? |
Exponential |
|
|
What happens during the stationary phase |
Reproduction and death dates are equal |
|
|
What happens during the death phase |
Number of dying sales exceeds the number of newly formed |
|
|
To determine a growth curve, samples from actively growing culture are taken over what period of time? |
24-48 hours |
|
|
What does an indirect method of measure measure? |
Turbidity of culture to track the changes as growth occurs |
|
|
What's the dilution factor is calculated, take the blank and multiply it by the number of colonies counted. |
Reciprocal |
|
|
What is a graphical representation of the change in population size over time? |
Growth curve |
|
|
What happens during the lag phase |
Bacteria is adapting to his new |
|
|
What happens during the logarithmic phase? |
Exponential |
|
|
What happens during the stationary phase |
Reproduction and death dates are equal |
|
|
What happens during the death phase |
Number of dying sales exceeds the number of newly formed |
|
|
To determine a growth curve, samples from actively growing culture are taken over what period of time? |
24-48 hours |
|
|
What does an indirect method of measure measure? |
Turbidity of culture to track the changes as growth occurs |
|
|
What is a spectro photo meter |
Tool used to measure cell turbidity by measuring the absorbance or optical density of a culture |
|
|
What's the dilution factor is calculated, take the blank and multiply it by the number of colonies counted. |
Reciprocal |
|
|
What is a graphical representation of the change in population size over time? |
Growth curve |
|
|
What happens during the lag phase |
Bacteria is adapting to his new |
|
|
What happens during the logarithmic phase? |
Exponential |
|
|
What happens during the stationary phase |
Reproduction and death dates are equal |
|
|
What happens during the death phase |
Number of dying sales exceeds the number of newly formed |
|
|
To determine a growth curve, samples from actively growing culture are taken over what period of time? |
24-48 hours |
|
|
What does an indirect method of measure measure? |
Turbidity of culture to track the changes as growth occurs |
|
|
What is a spectro photo meter |
Tool used to measure cell turbidity by measuring the absorbance or optical density of a culture |
|
|
The blank the absorbance, the greater concentration of bacteria cells |
Higher |
|
|
What's the dilution factor is calculated, take the blank and multiply it by the number of colonies counted. |
Reciprocal |
|
|
Typical bacteria growth curves are constructed by measuring ocular distance ate at a wave links of blank? |
600 nm |
|
|
What is a graphical representation of the change in population size over time? |
Growth curve |
|
|
What happens during the lag phase |
Bacteria is adapting to his new |
|
|
What happens during the logarithmic phase? |
Exponential |
|
|
What happens during the stationary phase |
Reproduction and death dates are equal |
|
|
What happens during the death phase |
Number of dying sales exceeds the number of newly formed |
|
|
To determine a growth curve, samples from actively growing culture are taken over what period of time? |
24-48 hours |
|
|
What does an indirect method of measure measure? |
Turbidity of culture to track the changes as growth occurs |
|
|
What is a spectro photo meter |
Tool used to measure cell turbidity by measuring the absorbance or optical density of a culture |
|
|
The blank the absorbance, the greater concentration of bacteria cells |
Higher |
|
|
What's the dilution factor is calculated, take the blank and multiply it by the number of colonies counted. |
Reciprocal |
|
|
Typical bacteria growth curves are constructed by measuring ocular distance ate at a wave links of blank? |
600 nm |
|
|
What two ways can I direct account to be obtained |
Coulter counter or a special slide called hemocytometer. |
|
|
What is a graphical representation of the change in population size over time? |
Growth curve |
|
|
What happens during the lag phase |
Bacteria is adapting to his new |
|
|
What happens during the logarithmic phase? |
Exponential |
|
|
What happens during the stationary phase |
Reproduction and death dates are equal |
|
|
What happens during the death phase |
Number of dying sales exceeds the number of newly formed |
|
|
To determine a growth curve, samples from actively growing culture are taken over what period of time? |
24-48 hours |
|
|
What does an indirect method of measure measure? |
Turbidity of culture to track the changes as growth occurs |
|
|
What is a spectro photo meter |
Tool used to measure cell turbidity by measuring the absorbance or optical density of a culture |
|
|
The blank the absorbance, the greater concentration of bacteria cells |
Higher |
|
|
What's the dilution factor is calculated, take the blank and multiply it by the number of colonies counted. |
Reciprocal |
|
|
Typical bacteria growth curves are constructed by measuring ocular distance ate at a wave links of blank? |
600 nm |
|
|
What two ways can I direct account to be obtained |
Coulter counter or a special slide called hemocytometer. |
|
|
What is a hemocytometer |
A slide which contains a grid that allows a person to count the number of cells in a known volume of fluid. |
|
|
What is a graphical representation of the change in population size over time? |
Growth curve |
|
|
What happens during the lag phase |
Bacteria is adapting to his new |
|
|
What happens during the logarithmic phase? |
Exponential |
|
|
What happens during the stationary phase |
Reproduction and death dates are equal |
|
|
What happens during the death phase |
Number of dying sales exceeds the number of newly formed |
|
|
To determine a growth curve, samples from actively growing culture are taken over what period of time? |
24-48 hours |
|
|
What does an indirect method of measure measure? |
Turbidity of culture to track the changes as growth occurs |
|
|
What is a spectro photo meter |
Tool used to measure cell turbidity by measuring the absorbance or optical density of a culture |
|
|
The blank the absorbance, the greater concentration of bacteria cells |
Higher |
|
|
What's the dilution factor is calculated, take the blank and multiply it by the number of colonies counted. |
Reciprocal |
|
|
Typical bacteria growth curves are constructed by measuring ocular distance ate at a wave links of blank? |
600 nm |
|
|
What two ways can I direct account to be obtained |
Coulter counter or a special slide called hemocytometer. |
|
|
What is a hemocytometer |
A slide which contains a grid that allows a person to count the number of cells in a known volume of fluid. |
|
|
What is a coulter counter |
Apparatus for accounting and sizing particles suspended in electrolytes |
|
|
What is a graphical representation of the change in population size over time? |
Growth curve |
|
|
What happens during the lag phase |
Bacteria is adapting to his new |
|
|
What happens during the logarithmic phase? |
Exponential |
|
|
What happens during the stationary phase |
Reproduction and death dates are equal |
|
|
What happens during the death phase |
Number of dying sales exceeds the number of newly formed |
|
|
To determine a growth curve, samples from actively growing culture are taken over what period of time? |
24-48 hours |
|
|
What does an indirect method of measure measure? |
Turbidity of culture to track the changes as growth occurs |
|
|
What is a spectro photo meter |
Tool used to measure cell turbidity by measuring the absorbance or optical density of a culture |
|
|
The blank the absorbance, the greater concentration of bacteria cells |
Higher |
|
|
What's the dilution factor is calculated, take the blank and multiply it by the number of colonies counted. |
Reciprocal |
|
|
Typical bacteria growth curves are constructed by measuring ocular distance ate at a wave links of blank? |
600 nm |
|
|
What two ways can I direct account to be obtained |
Coulter counter or a special slide called hemocytometer. |
|
|
What is a hemocytometer |
A slide which contains a grid that allows a person to count the number of cells in a known volume of fluid. |
|
|
What is a coulter counter |
Apparatus for accounting and sizing particles suspended in electrolytes |
|
|
What is a Kirby-Bauer antibiotic susceptibility test |
A standardize procedure using disc diffusion to determine the susceptibility of bacterial strain to an antibody. |
|
|
What is a graphical representation of the change in population size over time? |
Growth curve |
|
|
What happens during the lag phase |
Bacteria is adapting to his new |
|
|
What happens during the logarithmic phase? |
Exponential |
|
|
What happens during the stationary phase |
Reproduction and death dates are equal |
|
|
What happens during the death phase |
Number of dying sales exceeds the number of newly formed |
|
|
To determine a growth curve, samples from actively growing culture are taken over what period of time? |
24-48 hours |
|
|
What does an indirect method of measure measure? |
Turbidity of culture to track the changes as growth occurs |
|
|
What is a spectro photo meter |
Tool used to measure cell turbidity by measuring the absorbance or optical density of a culture |
|
|
The blank the absorbance, the greater concentration of bacteria cells |
Higher |
|
|
What's the dilution factor is calculated, take the blank and multiply it by the number of colonies counted. |
Reciprocal |
|
|
Typical bacteria growth curves are constructed by measuring ocular distance ate at a wave links of blank? |
600 nm |
|
|
What two ways can I direct account to be obtained |
Coulter counter or a special slide called hemocytometer. |
|
|
What is a hemocytometer |
A slide which contains a grid that allows a person to count the number of cells in a known volume of fluid. |
|
|
What is a coulter counter |
Apparatus for accounting and sizing particles suspended in electrolytes |
|
|
What is a Kirby-Bauer antibiotic susceptibility test |
A standardize procedure using disc diffusion to determine the susceptibility of bacterial strain to an antibody. |
|
|
The Kirby-Bauer antibiotics susceptibility test is a blank procedure to ensure accurate and reproducible results. |
Highly controlled |
|
|
What is a graphical representation of the change in population size over time? |
Growth curve |
|
|
What happens during the lag phase |
Bacteria is adapting to his new |
|
|
What happens during the logarithmic phase? |
Exponential |
|
|
What happens during the stationary phase |
Reproduction and death dates are equal |
|
|
What happens during the death phase |
Number of dying sales exceeds the number of newly formed |
|
|
To determine a growth curve, samples from actively growing culture are taken over what period of time? |
24-48 hours |
|
|
What does an indirect method of measure measure? |
Turbidity of culture to track the changes as growth occurs |
|
|
What is a spectro photo meter |
Tool used to measure cell turbidity by measuring the absorbance or optical density of a culture |
|
|
The blank the absorbance, the greater concentration of bacteria cells |
Higher |
|
|
What's the dilution factor is calculated, take the blank and multiply it by the number of colonies counted. |
Reciprocal |
|
|
Typical bacteria growth curves are constructed by measuring ocular distance ate at a wave links of blank? |
600 nm |
|
|
What two ways can I direct account to be obtained |
Coulter counter or a special slide called hemocytometer. |
|
|
What is a hemocytometer |
A slide which contains a grid that allows a person to count the number of cells in a known volume of fluid. |
|
|
What is a coulter counter |
Apparatus for accounting and sizing particles suspended in electrolytes |
|
|
What is a Kirby-Bauer antibiotic susceptibility test |
A standardize procedure using disc diffusion to determine the susceptibility of bacterial strain to an antibody. |
|
|
The Kirby-Bauer antibiotics susceptibility test is a blank procedure to ensure accurate and reproducible results. |
Highly controlled |
|
|
Which media is used when performing the Kirby-Bauer antibiotic susceptibility test? |
Mueller-Hinton auger |
|
|
What is a graphical representation of the change in population size over time? |
Growth curve |
|
|
What happens during the lag phase |
Bacteria is adapting to his new |
|
|
What happens during the logarithmic phase? |
Exponential |
|
|
What happens during the stationary phase |
Reproduction and death dates are equal |
|
|
What happens during the death phase |
Number of dying sales exceeds the number of newly formed |
|
|
To determine a growth curve, samples from actively growing culture are taken over what period of time? |
24-48 hours |
|
|
What does an indirect method of measure measure? |
Turbidity of culture to track the changes as growth occurs |
|
|
What is a spectro photo meter |
Tool used to measure cell turbidity by measuring the absorbance or optical density of a culture |
|
|
The blank the absorbance, the greater concentration of bacteria cells |
Higher |
|
|
What's the dilution factor is calculated, take the blank and multiply it by the number of colonies counted. |
Reciprocal |
|
|
Typical bacteria growth curves are constructed by measuring ocular distance ate at a wave links of blank? |
600 nm |
|
|
What two ways can I direct account to be obtained |
Coulter counter or a special slide called hemocytometer. |
|
|
What is a hemocytometer |
A slide which contains a grid that allows a person to count the number of cells in a known volume of fluid. |
|
|
What is a coulter counter |
Apparatus for accounting and sizing particles suspended in electrolytes |
|
|
What is a Kirby-Bauer antibiotic susceptibility test |
A standardize procedure using disc diffusion to determine the susceptibility of bacterial strain to an antibody. |
|
|
The Kirby-Bauer antibiotics susceptibility test is a blank procedure to ensure accurate and reproducible results. |
Highly controlled |
|
|
Which media is used when performing the Kirby-Bauer antibiotic susceptibility test? |
Mueller-Hinton auger |
|
|
What is the specific pH and uniform depth of the mueller-Hinton agar. |
PH 7.2-7.4; Depp (4MM) |
|
|
What is a graphical representation of the change in population size over time? |
Growth curve |
|
|
What happens during the lag phase |
Bacteria is adapting to his new |
|
|
What happens during the logarithmic phase? |
Exponential |
|
|
What happens during the stationary phase |
Reproduction and death dates are equal |
|
|
What happens during the death phase |
Number of dying sales exceeds the number of newly formed |
|
|
To determine a growth curve, samples from actively growing culture are taken over what period of time? |
24-48 hours |
|
|
What does an indirect method of measure measure? |
Turbidity of culture to track the changes as growth occurs |
|
|
What is a spectro photo meter |
Tool used to measure cell turbidity by measuring the absorbance or optical density of a culture |
|
|
The blank the absorbance, the greater concentration of bacteria cells |
Higher |
|
|
Standardized inoculum in Kirby-Bauer antibiotic susceptibility test is equivalent to? |
McFarland 0.5. Turbidity, which is approximately 1.5×10 to the eighth bacteria per milliliter |
|
|
Standardized inoculum in Kirby-Bauer antibiotic susceptibility test is equivalent to? |
McFarland 0.5. Turbidity, which is approximately 1.5×10 to the eighth bacteria per milliliter |
|
|
What is zone of inhibition? |
Area where bacteria has not grown. The size of the zone depends on how effective the antibiotic is at stopping the growth of bacteria. |
|
|
Standardized inoculum in Kirby-Bauer antibiotic susceptibility test is equivalent to? |
McFarland 0.5. Turbidity, which is approximately 1.5×10 to the eighth bacteria per milliliter |
|
|
What is zone of inhibition? |
Area where bacteria has not grown. The size of the zone depends on how effective the antibiotic is at stopping the growth of bacteria. |
|
|
What size messed the zone of inhibition be to be considered susceptible? |
Greater than 13mm. |
|
|
Standardized inoculum in Kirby-Bauer antibiotic susceptibility test is equivalent to? |
McFarland 0.5. Turbidity, which is approximately 1.5×10 to the eighth bacteria per milliliter |
|
|
What is zone of inhibition? |
Area where bacteria has not grown. The size of the zone depends on how effective the antibiotic is at stopping the growth of bacteria. |
|
|
What size messed the zone of inhibition be to be considered susceptible? |
Greater than 13mm. |
|
|
Define antiseptic and give two examples |
Chemical agent that can be applied to tissue. Does not kill in the spores; example: alcohol and Betadine |
|
|
Standardized inoculum in Kirby-Bauer antibiotic susceptibility test is equivalent to? |
McFarland 0.5. Turbidity, which is approximately 1.5×10 to the eighth bacteria per milliliter |
|
|
What is zone of inhibition? |
Area where bacteria has not grown. The size of the zone depends on how effective the antibiotic is at stopping the growth of bacteria. |
|
|
What size messed the zone of inhibition be to be considered susceptible? |
Greater than 13mm. |
|
|
Define antiseptic and give two examples |
Chemical agent that can be applied to tissue. Does not kill in the spores; example: alcohol and Betadine |
|
|
Define disinfectants and give example |
Chemical agents applied to in eight objects which are more harsh. Somebody destroyed endospores. Example: ethylene oxide |
|
|
Standardized inoculum in Kirby-Bauer antibiotic susceptibility test is equivalent to? |
McFarland 0.5. Turbidity, which is approximately 1.5×10 to the eighth bacteria per milliliter |
|
|
What is zone of inhibition? |
Area where bacteria has not grown. The size of the zone depends on how effective the antibiotic is at stopping the growth of bacteria. |
|
|
What size messed the zone of inhibition be to be considered susceptible? |
Greater than 13mm. |
|
|
Define antiseptic and give two examples |
Chemical agent that can be applied to tissue. Does not kill in the spores; example: alcohol and Betadine |
|
|
Define disinfectants and give example |
Chemical agents applied to in eight objects which are more harsh. Somebody destroyed endospores. Example: ethylene oxide |
|
|
Define sanitizer |
Agent if used to reduce microbial growth but do not kill all microbes. Used in the food industry to clean getting a Quitman |
|
|
Standardized inoculum in Kirby-Bauer antibiotic susceptibility test is equivalent to? |
McFarland 0.5. Turbidity, which is approximately 1.5×10 to the eighth bacteria per milliliter |
|
|
What is zone of inhibition? |
Area where bacteria has not grown. The size of the zone depends on how effective the antibiotic is at stopping the growth of bacteria. |
|
|
What size messed the zone of inhibition be to be considered susceptible? |
Greater than 13mm. |
|
|
Define antiseptic and give two examples |
Chemical agent that can be applied to tissue. Does not kill in the spores; example: alcohol and Betadine |
|
|
Define disinfectants and give example |
Chemical agents applied to in eight objects which are more harsh. Somebody destroyed endospores. Example: ethylene oxide |
|
|
Define sanitizer |
Agent if used to reduce microbial growth but do not kill all microbes. Used in the food industry to clean getting a Quitman |
|
|
Define bacteriostatic |
Agent that inhibits the growth of bacteria, but does not kill them. |
|
|
Standardized inoculum in Kirby-Bauer antibiotic susceptibility test is equivalent to? |
McFarland 0.5. Turbidity, which is approximately 1.5×10 to the eighth bacteria per milliliter |
|
|
What is zone of inhibition? |
Area where bacteria has not grown. The size of the zone depends on how effective the antibiotic is at stopping the growth of bacteria. |
|
|
What size messed the zone of inhibition be to be considered susceptible? |
Greater than 13mm. |
|
|
Define antiseptic and give two examples |
Chemical agent that can be applied to tissue. Does not kill in the spores; example: alcohol and Betadine |
|
|
Define disinfectants and give example |
Chemical agents applied to in eight objects which are more harsh. Somebody destroyed endospores. Example: ethylene oxide |
|
|
Define sanitizer |
Agent if used to reduce microbial growth but do not kill all microbes. Used in the food industry to clean getting a Quitman |
|
|
Define bacteriostatic |
Agent that inhibits the growth of bacteria, but does not kill them. |
|
|
Define bacteriocidal |
Agent that kills bacterial |
|
|
Standardized inoculum in Kirby-Bauer antibiotic susceptibility test is equivalent to? |
McFarland 0.5. Turbidity, which is approximately 1.5×10 to the eighth bacteria per milliliter |
|
|
What is zone of inhibition? |
Area where bacteria has not grown. The size of the zone depends on how effective the antibiotic is at stopping the growth of bacteria. |
|
|
What size messed the zone of inhibition be to be considered susceptible? |
Greater than 13mm. |
|
|
Define antiseptic and give two examples |
Chemical agent that can be applied to tissue. Does not kill in the spores; example: alcohol and Betadine |
|
|
Define disinfectants and give example |
Chemical agents applied to in eight objects which are more harsh. Somebody destroyed endospores. Example: ethylene oxide |
|
|
Define sanitizer |
Agent if used to reduce microbial growth but do not kill all microbes. Used in the food industry to clean getting a Quitman |
|
|
Define bacteriostatic |
Agent that inhibits the growth of bacteria, but does not kill them. |
|
|
Define bacteriocidal |
Agent that kills bacterial |
|
|
What is a bacteriophage |
Virus that infects bacteria; most common biological entity on earth. Commonly called a phage. |
|
|
What is the genetic material that bacterial phages contain in our Protane capsid |
ssRNA, DSR and A, ssDNA, dsRNA |
|
|
What is the genetic material that bacterial phages contain in our Protane capsid |
ssRNA, DSR and A, ssDNA, dsRNA |
|
|
What are estimated to be the most widely distributed and diverse entities in the biosphere |
Phages |
|
|
What is the genetic material that bacterial phages contain in our Protane capsid |
ssRNA, DSR and A, ssDNA, dsRNA |
|
|
What are estimated to be the most widely distributed and diverse entities in the biosphere |
Phages |
|
|
What is one of the densest natural resources for phages and other viruses? |
Seawater. |
|
|
What is the genetic material that bacterial phages contain in our Protane capsid |
ssRNA, DSR and A, ssDNA, dsRNA |
|
|
What are estimated to be the most widely distributed and diverse entities in the biosphere |
Phages |
|
|
What is one of the densest natural resources for phages and other viruses? |
Sea water |
|
|
Up to what percentage of marine bacteria may be infected by phages? |
70% |
|
|
What are lysozymes |
Enzymes which destroys cell walls and are found in saliva, human milk, and mucus. Also found an egg whites. |
|
|
What are lysozymes |
Enzymes which destroys cell walls and are found in saliva, human milk, and mucus. Also found an egg whites. |
|
|
Which bacteria are typically more susceptible to the effects of lysozyme? |
Gram-positive |
|
|
What are lysozymes |
Enzymes which destroys cell walls and are found in saliva, human milk, and mucus. Also found an egg whites. |
|
|
Which bacteria are typically more susceptible to the effects of lysozyme? |
Gram-positive |
|
|
What prevents the last design from reaching the peptidoglycan layer in Graham negative bacteria? |
Outer membrane. |
|
|
What are lysozymes |
Enzymes which destroys cell walls and are found in saliva, human milk, and mucus. Also found an egg whites. |
|
|
Which bacteria are typically more susceptible to the effects of lysozyme? |
Gram-positive |
|
|
What prevents the last design from reaching the peptidoglycan layer in Graham negative bacteria? |
Outer membrane. |
|
|
Which gram-positive bacteria is the exception, and is not sensitive to the effects of lights is on? |
Staphylococcus aureus |
|
|
What are lysozymes |
Enzymes which destroys cell walls and are found in saliva, human milk, and mucus. Also found an egg whites. |
|
|
Which bacteria are typically more susceptible to the effects of lysozyme? |
Gram-positive |
|
|
What prevents the last design from reaching the peptidoglycan layer in Graham negative bacteria? |
Outer membrane. |
|
|
Which gram-positive bacteria is the exception, and is not sensitive to the effects of lights is on? |
Staphylococcus aureus |
|
|
What, in the cell Wall of staphylococcus aureus makes it less sensitive to lysosome? |
Teichoic acid |
|
|
What are lysozymes |
Enzymes which destroys cell walls and are found in saliva, human milk, and mucus. Also found an egg whites. |
|
|
Which bacteria are typically more susceptible to the effects of lysozyme? |
Gram-positive |
|
|
What prevents the last design from reaching the peptidoglycan layer in Graham negative bacteria? |
Outer membrane. |
|
|
Which gram-positive bacteria is the exception, and is not sensitive to the effects of lights is on? |
Staphylococcus aureus |
|
|
What, in the cell Wall of staphylococcus aureus makes it less sensitive to lysosome? |
Teichoic acid |
|
|
What type of tests maybe used to determine the presence of a particular antigen |
serological |
|
|
What are lysozymes |
Enzymes which destroys cell walls and are found in saliva, human milk, and mucus. Also found an egg whites. |
|
|
Which bacteria are typically more susceptible to the effects of lysozyme? |
Gram-positive |
|
|
What prevents the last design from reaching the peptidoglycan layer in Graham negative bacteria? |
Outer membrane. |
|
|
Which gram-positive bacteria is the exception, and is not sensitive to the effects of lights is on? |
Staphylococcus aureus |
|
|
What, in the cell Wall of staphylococcus aureus makes it less sensitive to lysosome? |
Teichoic acid |
|
|
What type of tests maybe used to determine the presence of a particular antigen |
serological |
|
|
What are antigens made of? |
Primarily proteins but may also be carbohydrates, lipids, nucleic acid's. |
|
|
What are lysozymes |
Enzymes which destroys cell walls and are found in saliva, human milk, and mucus. Also found an egg whites. |
|
|
Which bacteria are typically more susceptible to the effects of lysozyme? |
Gram-positive |
|
|
What prevents the last design from reaching the peptidoglycan layer in Graham negative bacteria? |
Outer membrane. |
|
|
Which gram-positive bacteria is the exception, and is not sensitive to the effects of lights is on? |
Staphylococcus aureus |
|
|
What, in the cell Wall of staphylococcus aureus makes it less sensitive to lysosome? |
Teichoic acid |
|
|
What type of tests maybe used to determine the presence of a particular antigen |
serological |
|
|
What are antigens made of? |
Primarily proteins but may also be carbohydrates, lipids, nucleic acid's. |
|
|
What is the purpose of a catalase test |
Differentiates bacteria based on the presence of enzyme catalase which breaks down hydrogen peroxide into water and oxygen. |
|
|
What are lysozymes |
Enzymes which destroys cell walls and are found in saliva, human milk, and mucus. Also found an egg whites. |
|
|
Which bacteria are typically more susceptible to the effects of lysozyme? |
Gram-positive |
|
|
What prevents the last design from reaching the peptidoglycan layer in Graham negative bacteria? |
Outer membrane. |
|
|
Which gram-positive bacteria is the exception, and is not sensitive to the effects of lights is on? |
Staphylococcus aureus |
|
|
What, in the cell Wall of staphylococcus aureus makes it less sensitive to lysosome? |
Teichoic acid |
|
|
What type of tests maybe used to determine the presence of a particular antigen |
serological |
|
|
What are antigens made of? |
Primarily proteins but may also be carbohydrates, lipids, nucleic acid's. |
|
|
What is the purpose of a catalase test |
Differentiates bacteria based on the presence of enzyme catalase which breaks down hydrogen peroxide into water and oxygen. |
|
|
What is a catalase test used to differentiate between? |
Streptococcus and staphylococcus |
|
|
What are lysozymes |
Enzymes which destroys cell walls and are found in saliva, human milk, and mucus. Also found an egg whites. |
|
|
Which bacteria are typically more susceptible to the effects of lysozyme? |
Gram-positive |
|
|
What prevents the last design from reaching the peptidoglycan layer in Graham negative bacteria? |
Outer membrane. |
|
|
Which gram-positive bacteria is the exception, and is not sensitive to the effects of lights is on? |
Staphylococcus aureus |
|
|
What, in the cell Wall of staphylococcus aureus makes it less sensitive to lysosome? |
Teichoic acid |
|
|
What type of tests maybe used to determine the presence of a particular antigen |
serological |
|
|
What are antigens made of? |
Primarily proteins but may also be carbohydrates, lipids, nucleic acid's. |
|
|
What is the purpose of a catalase test |
Differentiates bacteria based on the presence of enzyme catalase which breaks down hydrogen peroxide into water and oxygen. |
|
|
What is a catalase test used to differentiate between? |
Streptococcus and staphylococcus |
|
|
What indicates a positive catalase test? |
Bubbling |
|
|
What are lysozymes |
Enzymes which destroys cell walls and are found in saliva, human milk, and mucus. Also found an egg whites. |
|
|
Oxidase test |
Differentiates bacteria based on presence of cytochrome C oxidase. |
|
|
Which bacteria are typically more susceptible to the effects of lysozyme? |
Gram-positive |
|
|
What prevents the last design from reaching the peptidoglycan layer in Graham negative bacteria? |
Outer membrane. |
|
|
Which gram-positive bacteria is the exception, and is not sensitive to the effects of lights is on? |
Staphylococcus aureus |
|
|
What, in the cell Wall of staphylococcus aureus makes it less sensitive to lysosome? |
Teichoic acid |
|
|
What type of tests maybe used to determine the presence of a particular antigen |
serological |
|
|
What are antigens made of? |
Primarily proteins but may also be carbohydrates, lipids, nucleic acid's. |
|
|
What is the purpose of a catalase test |
Differentiates bacteria based on the presence of enzyme catalase which breaks down hydrogen peroxide into water and oxygen. |
|
|
What is a catalase test used to differentiate between? |
Streptococcus and staphylococcus |
|
|
What indicates a positive catalase test? |
Bubbling |
|
|
What are lysozymes |
Enzymes which destroys cell walls and are found in saliva, human milk, and mucus. Also found an egg whites. |
|
|
Oxidase test |
Differentiates bacteria based on presence of cytochrome C oxidase. |
|
|
What are the results of an oxidase test? |
Purple is positive; yellow is negative |
|
|
Which bacteria are typically more susceptible to the effects of lysozyme? |
Gram-positive |
|
|
What prevents the last design from reaching the peptidoglycan layer in Graham negative bacteria? |
Outer membrane. |
|
|
Which gram-positive bacteria is the exception, and is not sensitive to the effects of lights is on? |
Staphylococcus aureus |
|
|
What, in the cell Wall of staphylococcus aureus makes it less sensitive to lysosome? |
Teichoic acid |
|
|
What type of tests maybe used to determine the presence of a particular antigen |
serological |
|
|
What are antigens made of? |
Primarily proteins but may also be carbohydrates, lipids, nucleic acid's. |
|
|
What is the purpose of a catalase test |
Differentiates bacteria based on the presence of enzyme catalase which breaks down hydrogen peroxide into water and oxygen. |
|
|
What is a catalase test used to differentiate between? |
Streptococcus and staphylococcus |
|
|
What indicates a positive catalase test? |
Bubbling |
|
|
What are lysozymes |
Enzymes which destroys cell walls and are found in saliva, human milk, and mucus. Also found an egg whites. |
|
|
Oxidase test |
Differentiates bacteria based on presence of cytochrome C oxidase. |
|
|
What are the results of an oxidase test? |
Purple is positive; yellow is negative |
|
|
What is the purpose of 3% KOH |
Verifies Gram stain. |
|
|
Which bacteria are typically more susceptible to the effects of lysozyme? |
Gram-positive |
|
|
What prevents the last design from reaching the peptidoglycan layer in Graham negative bacteria? |
Outer membrane. |
|
|
Which gram-positive bacteria is the exception, and is not sensitive to the effects of lights is on? |
Staphylococcus aureus |
|
|
What, in the cell Wall of staphylococcus aureus makes it less sensitive to lysosome? |
Teichoic acid |
|
|
What type of tests maybe used to determine the presence of a particular antigen |
serological |
|
|
What are antigens made of? |
Primarily proteins but may also be carbohydrates, lipids, nucleic acid's. |
|
|
What is the purpose of a catalase test |
Differentiates bacteria based on the presence of enzyme catalase which breaks down hydrogen peroxide into water and oxygen. |
|
|
What is a catalase test used to differentiate between? |
Streptococcus and staphylococcus |
|
|
What indicates a positive catalase test? |
Bubbling |
|
|
What are lysozymes |
Enzymes which destroys cell walls and are found in saliva, human milk, and mucus. Also found an egg whites. |
|
|
Oxidase test |
Differentiates bacteria based on presence of cytochrome C oxidase. |
|
|
What are the results of an oxidase test? |
Purple is positive; yellow is negative |
|
|
What is the purpose of 3% KOH |
Verifies Gram stain. |
|
|
What are the results of 3% KOH? |
Positive is stringy viscous on Graham negative; negative no reaction on gram-positive |
|
|
Which bacteria are typically more susceptible to the effects of lysozyme? |
Gram-positive |
|
|
What prevents the last design from reaching the peptidoglycan layer in Graham negative bacteria? |
Outer membrane. |
|
|
Which gram-positive bacteria is the exception, and is not sensitive to the effects of lights is on? |
Staphylococcus aureus |
|
|
What, in the cell Wall of staphylococcus aureus makes it less sensitive to lysosome? |
Teichoic acid |
|
|
What type of tests maybe used to determine the presence of a particular antigen |
serological |
|
|
What are antigens made of? |
Primarily proteins but may also be carbohydrates, lipids, nucleic acid's. |
|
|
What is the purpose of a catalase test |
Differentiates bacteria based on the presence of enzyme catalase which breaks down hydrogen peroxide into water and oxygen. |
|
|
What is a catalase test used to differentiate between? |
Streptococcus and staphylococcus |
|
|
What indicates a positive catalase test? |
Bubbling |
|
|
What are lysozymes |
Enzymes which destroys cell walls and are found in saliva, human milk, and mucus. Also found an egg whites. |
|
|
Oxidase test |
Differentiates bacteria based on presence of cytochrome C oxidase. |
|
|
What are the results of an oxidase test? |
Purple is positive; yellow is negative |
|
|
What is the purpose of 3% KOH |
Verifies Gram stain. |
|
|
What are the results of 3% KOH? |
Positive is stringy viscous on Graham negative; negative no reaction on gram-positive |
|
|
0F tubes are used to determine what? |
If I microorganism confirm it and/or oxidize a carbohydrate. |
|
|
Which bacteria are typically more susceptible to the effects of lysozyme? |
Gram-positive |
|
|
What prevents the last design from reaching the peptidoglycan layer in Graham negative bacteria? |
Outer membrane. |
|
|
Which gram-positive bacteria is the exception, and is not sensitive to the effects of lights is on? |
Staphylococcus aureus |
|
|
What, in the cell Wall of staphylococcus aureus makes it less sensitive to lysosome? |
Teichoic acid |
|
|
What type of tests maybe used to determine the presence of a particular antigen |
serological |
|
|
What are antigens made of? |
Primarily proteins but may also be carbohydrates, lipids, nucleic acid's. |
|
|
What is the purpose of a catalase test |
Differentiates bacteria based on the presence of enzyme catalase which breaks down hydrogen peroxide into water and oxygen. |
|
|
What is a catalase test used to differentiate between? |
Streptococcus and staphylococcus |
|
|
What indicates a positive catalase test? |
Bubbling |
|
|
What are lysozymes |
Enzymes which destroys cell walls and are found in saliva, human milk, and mucus. Also found an egg whites. |
|
|
Oxidase test |
Differentiates bacteria based on presence of cytochrome C oxidase. |
|
|
What are the results of an oxidase test? |
Purple is positive; yellow is negative |
|
|
What is the purpose of 3% KOH |
Verifies Gram stain. |
|
|
What are the results of 3% KOH? |
Positive is stringy viscous on Graham negative; negative no reaction on gram-positive |
|
|
0F tubes are used to determine what? |
If I microorganism confirm it and/or oxidize a carbohydrate. |
|
|
What is a positive result on OF tubes |
Media turns from green to yellow. |
|
|
Which bacteria are typically more susceptible to the effects of lysozyme? |
Gram-positive |
|
|
What prevents the last design from reaching the peptidoglycan layer in Graham negative bacteria? |
Outer membrane. |
|
|
Which gram-positive bacteria is the exception, and is not sensitive to the effects of lights is on? |
Staphylococcus aureus |
|
|
What, in the cell Wall of staphylococcus aureus makes it less sensitive to lysosome? |
Teichoic acid |
|
|
What type of tests maybe used to determine the presence of a particular antigen |
serological |
|
|
What are antigens made of? |
Primarily proteins but may also be carbohydrates, lipids, nucleic acid's. |
|
|
What is the purpose of a catalase test |
Differentiates bacteria based on the presence of enzyme catalase which breaks down hydrogen peroxide into water and oxygen. |
|
|
What is a catalase test used to differentiate between? |
Streptococcus and staphylococcus |
|
|
What indicates a positive catalase test? |
Bubbling |
|
|
What are lysozymes |
Enzymes which destroys cell walls and are found in saliva, human milk, and mucus. Also found an egg whites. |
|
|
Oxidase test |
Differentiates bacteria based on presence of cytochrome C oxidase. |
|
|
What are the results of an oxidase test? |
Purple is positive; yellow is negative |
|
|
What is the purpose of 3% KOH |
Verifies Gram stain. |
|
|
What are the results of 3% KOH? |
Positive is stringy viscous on Graham negative; negative no reaction on gram-positive |
|
|
0F tubes are used to determine what? |
If I microorganism confirm it and/or oxidize a carbohydrate. |
|
|
What is a positive result on OF tubes |
Media turns from green to yellow. |
|
|
With oil in the 0F tubes, what color is positive for |
Yellow |
|
|
Which bacteria are typically more susceptible to the effects of lysozyme? |
Gram-positive |
|
|
What prevents the last design from reaching the peptidoglycan layer in Graham negative bacteria? |
Outer membrane. |
|
|
Which gram-positive bacteria is the exception, and is not sensitive to the effects of lights is on? |
Staphylococcus aureus |
|
|
What, in the cell Wall of staphylococcus aureus makes it less sensitive to lysosome? |
Teichoic acid |
|
|
What type of tests maybe used to determine the presence of a particular antigen |
serological |
|
|
What are antigens made of? |
Primarily proteins but may also be carbohydrates, lipids, nucleic acid's. |
|
|
What is the purpose of a catalase test |
Differentiates bacteria based on the presence of enzyme catalase which breaks down hydrogen peroxide into water and oxygen. |
|
|
What is a catalase test used to differentiate between? |
Streptococcus and staphylococcus |
|
|
What indicates a positive catalase test? |
Bubbling |
|
|
What is the Kirby-Bauer antibiotic susceptibility test used for? |
To determine the susceptibility of a bacterial strain to an antibiotic |
|
|
What is the Kirby-Bauer antibiotic susceptibility test used for? |
To determine the susceptibility of a bacterial strain to an antibiotic |
|
|
What type of agar is used for a Kirby Bauer test? |
Mueller-Hinton agar |
|
|
What is the Kirby-Bauer antibiotic susceptibility test used for? |
To determine the susceptibility of a bacterial strain to an antibiotic |
|
|
What type of agar is used for a Kirby Bauer test? |
Mueller-Hinton agar |
|
|
What is the pH of Mueller-Hinton agar |
7.2-7.4 |
|
|
What is the Kirby-Bauer antibiotic susceptibility test used for? |
To determine the susceptibility of a bacterial strain to an antibiotic |
|
|
What type of agar is used for a Kirby Bauer test? |
Mueller-Hinton agar |
|
|
What is the pH of Mueller-Hinton agar |
7.2-7.4 |
|
|
What makes the Kirby-Bauer test highly controlled? |
Specific media used, standardized inoculum, and known potency of antibiotic disks |
|
|
What is the Kirby-Bauer antibiotic susceptibility test used for? |
To determine the susceptibility of a bacterial strain to an antibiotic |
|
|
What type of agar is used for a Kirby Bauer test? |
Mueller-Hinton agar |
|
|
What is the pH of Mueller-Hinton agar |
7.2-7.4 |
|
|
What makes the Kirby-Bauer test highly controlled? |
Specific media used, standardized inoculum, and known potency of antibiotic disks |
|
|
What size zone of inhibition indicates susceptible? |
Greater than 14 mm |
|
|
What is the Kirby-Bauer antibiotic susceptibility test used for? |
To determine the susceptibility of a bacterial strain to an antibiotic |
|
|
What type of agar is used for a Kirby Bauer test? |
Mueller-Hinton agar |
|
|
What is the pH of Mueller-Hinton agar |
7.2-7.4 |
|
|
What makes the Kirby-Bauer test highly controlled? |
Specific media used, standardized inoculum, and known potency of antibiotic disks |
|
|
What size zone of inhibition indicates susceptible? |
Greater than 14 mm |
|
|
What does zone of inhibition between 10-14 indicate? |
Intermediate susceptibility |
|
|
What is the Kirby-Bauer antibiotic susceptibility test used for? |
To determine the susceptibility of a bacterial strain to an antibiotic |
|
|
What type of agar is used for a Kirby Bauer test? |
Mueller-Hinton agar |
|
|
What is the pH of Mueller-Hinton agar |
7.2-7.4 |
|
|
What makes the Kirby-Bauer test highly controlled? |
Specific media used, standardized inoculum, and known potency of antibiotic disks |
|
|
What size zone of inhibition indicates susceptible? |
Greater than 14 mm |
|
|
What does zone of inhibition between 10-14 indicate? |
Intermediate susceptibility |
|
|
What does the zone of inhibition less than 10 indicate |
Resistant to the antibiotic |
|
|
What is the Kirby-Bauer antibiotic susceptibility test used for? |
To determine the susceptibility of a bacterial strain to an antibiotic |
|
|
What type of agar is used for a Kirby Bauer test? |
Mueller-Hinton agar |
|
|
What is the pH of Mueller-Hinton agar |
7.2-7.4 |
|
|
What makes the Kirby-Bauer test highly controlled? |
Specific media used, standardized inoculum, and known potency of antibiotic disks |
|
|
What size zone of inhibition indicates susceptible? |
Greater than 14 mm |
|
|
What does zone of inhibition between 10-14 indicate? |
Intermediate susceptibility |
|
|
What does the zone of inhibition less than 10 indicate |
Resistant to the antibiotic |
|
|
What was the name of the dispenser used to place the antibiotic disks up on the medium? |
Bumblebee |
|
|
What is the name of the gaseous disinfectant which may destroy endospores? |
Ethylene oxide |
