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55 Cards in this Set

  • Front
  • Back

Pathogen

An organism capable of disease

Inoculate

To transfer organisms to a medium to initiate growth

Sterile

Free of microorganisms and viruses

Ubiquity

The existence of something everywhere at the same time

Alpha hemolytic

A partial clearing of hemolysis surrounding colonies grown on blood agar plates often appears green

Beta hemolytic

A sharply defined clear zone of hemolysis surrounding colonies grown on blood agar

Catalase

An enzyme found in most aerobic organisms that breaks down H2O2 to water and oxygen

Enterococcui

Cocci found in the intestines

Gamma hemolysic

No blood cells are lysed on blood agar plate so it appears red

Glomerulonsphritis

Inflammation of the kidneys affecting the structure of the renal glomeruli (inflammation of the capillaries caused by toxins produced elsewhere in the body)

Inhibition zone

Lack bacterial growth around an antibiotic disc. The clear zone diameter is a measured in mm. It's where bacteria were killed

Inhibition zone

Lack bacterial growth around an antibiotic disc. The clear zone diameter is a measured in mm. It's where bacteria were killed

Nonsnterocci

Cocci found in other locations besides the intestines

Inhibition zone

Lack bacterial growth around an antibiotic disc. The clear zone diameter is a measured in mm. It's where bacteria were killed

Nonsnterocci

Cocci found in other locations besides the intestines

Nutrient Agar

Complex culture media nutritious with beef extract pepton (digestive protein)


17-20 mL of nutrient agar in each plate


2.5% agar is added to nutrient broth to solidify


Solid at 37 degrees Celsius

Autoclave

121 degree Celsius and 15 PSI sterilize the culture media with the temperature and with the pressure

Autoclave

121 degree Celsius and 15 PSI sterilize the culture media with the temperature and with the pressure

Moraxella catarrhails


Gram negative


Cat positive


Ox positive

Gram positive


Cat negative


Ox negative


Streptococcus

Corynebacterium and diphtheroids


Gram positive


Cat positive


Ox negative

Staphylococcus


Gram positive


Cat positive


Ox negative

Yeast


Appear gram positive


Cat positive


Ox positive

Normal biota

The organisms usually found associated with parts of the body (previously termed normal flora)

Moraxella catarrhails


Gram negative


Cat positive


Ox positive

Gram positive


Cat negative


Ox negative


Streptococcus

Corynebacterium and diphtheroids


Gram positive


Cat positive


Ox negative

Staphylococcus


Gram positive


Cat positive


Ox negative

Yeast


Appear gram positive


Cat positive


Ox positive

Encapsulation

A protective gelatinous material surrounding some bacteria that may also relate to their virulence

Oxidase

A reagent that test for cytochrome C. This reagent reacts with the air so it must be read within a minute to avoid a false positive test


Positive test=purple

Oxygen-Labile hemolysin

Hemolysin destroyed by oxygen, this is why the agar is stabbed, to check for oxygen labile hemolysins that would not be decayed otherwise

Example of beta hemolysic

Stapholoccus arueus

Purple


Green

Purple gamma


Green alpha

Beta, alpha and gamma

Beta and gamma

Escherichia coli

What are the different elevation?

Flat, conical, Craterlike

Staphylococcus epidermis

What is the log phase?

Where the bacteria is actively growing

Pseudomonas

Bacterlostatic

Prevented from growing but doesn't kill them

Serratia

Why isn't there one kind of agar medium that will support the growth of all microorganisms?

Because microorganisms are present everywhere so there is no one condition medium that will roemit the growth of all

Beta, alpha and gamma

Why do you invert agar plates when placing them in the incubator?

To prevent condensation from forming on the lid and dripping on the agar below. The moisture would permit colonies of bastetja to run together

How does the microscope zoom?

Once with the ocular lense 4x and once with the scanning lense 10x for a total of 40x

Beta and gamma

Escherichia coli

Escherichia coli

Color: goldish brown


Surface: shiny


Conskdtancy: moist


Elevation: conical

Staphylococcus epidermis

Staphylococcus epidermis

Color: pale yellow


Dull


Comical


Dry

Pseudomonas

Pseudomonas

Consistency: mucoid


Color: white/pale yellow


Surface: shiny


Elevation: flat

Serratia