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30 Cards in this Set
- Front
- Back
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What ingredients are in media?
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water, amino acids, vitamins, growth nutrients
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What are the two types of media? Describe.
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1. broth- liquid form, test tubes
2. agar- w/ other ingredients to form a solid medium (slants and plates) |
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How hot is the autoclave? Pressure?
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121 degrees C; 15 psi
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How long do you keep glassware in the autoclave? Media?
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30 minutes; 20 minutes
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what is the general medium we use to grow organisms?
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Tryptic soy: agar or broth (TSA or TSB)
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How do you make TSA?
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40 grams of powder to 1 liter of water
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What does epidemiology mean?
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study of factors affecting the health and illness of populations
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What three things does spreading disease depend on?
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1. Pathogen
2. Host/Carrier 3. Environment |
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What is a carrier? A source?
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organism that has a pathogen but shows no symptoms; has pathogen and shows symptoms
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What is biosafety?
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Containment of an organism
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Describe 4 levels of biosafety. What is our lab considered? What do we actually use?
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1. Biosafety level 1- nonpathogenic, aseptic technique
2. BSL2- pathogenic, may harm you-possible antibiotic use 3. BSL3- infectious agent; wear masks keep door closed; tuberculosis 4. BSL4- most infectious, not many in US |
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What are the three rules for general biosafety?
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1. Containment
2. Protect yourself 3. Protect organism |
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What does septic mean? Aspetic?
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contamination or infection; NO contamination
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Describe 4 levels of biosafety. What is our lab considered? What do we actually use?
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1. Biosafety level 1- nonpathogenic, aseptic technique
2. BSL2- pathogenic, may harm you-possible antibiotic use 3. BSL3- infectious agent; wear masks keep door closed; tuberculosis 4. BSL4- most infectious, not many in US |
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What are the three rules for general biosafety?
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1. Containment
2. Protect yourself 3. Protect organism |
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What does septic mean? Aspetic?
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contamination or infection; NO contamination
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Describe 4 levels of biosafety. What is our lab considered? What do we actually use?
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1. Biosafety level 1- nonpathogenic, aseptic technique
2. BSL2- pathogenic, may harm you-possible antibiotic use 3. BSL3- infectious agent; wear masks keep door closed; tuberculosis 4. BSL4- most infectious, not many in US |
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What are the three rules for general biosafety?
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1. Containment
2. Protect yourself 3. Protect organism |
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What does septic mean? Aspetic?
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contamination or infection; NO contamination
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Microorganisms are ubiquitous. What does ubiquity mean?
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They are found everywhere
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What is an opportunistic pathogen?
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An opportunistic infection is an infection caused by pathogens that usually do not cause disease in a healthy immune system. A compromised immune system, however, presents an "opportunity" for the pathogen to infect.
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What is similar in all organisms in a colony?
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same size, shape, and color
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What are the 5 categories of colony morphology?
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1. shape
2. margin 3. elevation 4. texture 5. pigmentation |
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4 different growths on slants. Which ones are non-motile?
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1. filiform- nonmotile
2. friable- nonmotile 3. spreading edge 4. translucent |
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4 different growths on broths.
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1. pellicle
2. sediment 3. spreading edge 4. flocculent |
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What does aerotolerance mean?
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how organism tolerants oxygen
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What are the three temperature-mediated factors? Explain each.
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1. psychophile- grows in temps <15C
2. mesophile- grows in temps 15C-40C 3. thermophile- grows in temps >40C |
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What are the 4 categories of oxygen requirements. Explain each.
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1. aerobe- uses oxygen to grow
2. anaerobe- does not grow in presence of oxygen 3. facultative- grows with or without oxygen 4. microaerophile- requires O2 at a specific atmospheric concentration |
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What is an obligate aerobe?
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Can't grow at all without oxygen
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What does FTM stand for? What does it consist of?
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Fluid Thyoglycollate Medium; consists of yeast extract, pancreatic digest of casein, sodium thyoglycollate acid, L-cystine, agar, and methylene blue
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