Is-Page 3 Part 2

Front 1

other examples of secondary/peripheral organs

Back 1

1. tonsils

2. appendix

3. peyer’s patches of intestine

4. mucosal-associated lymphoid tissue (MALT)

5. cutaneous-associated lymphoid tissue (CALT)

Front 2

rapid and convenient technique for generating immunophenotypic data of cells

Back 2

flow cytometry

Front 3

flow cytometry is useful for the evaluation of:

Back 3

1. leukemia

2. lymphoma

3. immunodeficiency

Front 4

flow cytometry is based on______derived from the suspension of particles of a flowing stream of fluid

Back 4

light scattering

Front 5

flow cytometry is based on______derived from the suspension of particles of a flowing stream of fluid

Back 5

light scattering

Front 6

fluidics used by flow cytometry where cells are suspended

Back 6

isotonic saline (sheath fluid)

Front 7

flow cytometry is based on______derived from the suspension of particles of a flowing stream of fluid

Back 7

light scattering

Front 8

fluidics used by flow cytometry where cells are suspended

Back 8

isotonic saline (sheath fluid)

Front 9

laser light source of flow cytometry

Back 9

solid state diode lasers

Front 10

indicator of cell size

Back 10

Forward Angle/Light Scatter (FSC)

Front 11

indicator of cell size

Back 11

Forward Angle/Light Scatter (FSC)

Front 12

indicator of granularity/intracellular complexity

Back 12

Right Angle/Side Scatter (SSC)

Front 13

ALL

Back 13

↑CD 10

Front 14

AML

Back 14

Î CD 34

Front 15

AML

Back 15

Î CD 34

Front 16

most common fluorescent labeled antibodies

Back 16

fluorescein isothiocyanate (FITC)

Front 17

added to quantify cell subpopulation or cell components

Back 17

fluorescent labeled antibodies

Front 18

added to quantify cell subpopulation or cell components

Back 18

fluorescent labeled antibodies

Front 19

identifies surface and cytoplasmic antigen expression

Back 19

fluorescent labeled antibodies

Front 20

signals in FSC is detected by

Back 20

photodiode

Front 21

signals in FSC is detected by

Back 21

photodiode

Front 22

signals of fluorescence is detected by

Back 22

photomultiplier tubes

Front 23

specimen for flow cytometry

Back 23

1. Whole Blood

2. Bone marrow

3. Body fluid aspirates

4. tissue sample

Front 24

anticoagulant of choice in flow cytometry

Back 24

1. EDTA

2. Heparin

3. Acid citrate dextrose

Front 25

what is remove in the specimen prior to testing in flow cytometry

Back 25

RBCs

Front 26

uses Ficoll-Hypaque solution

Back 26

density gradient solution

Hint 26

contains sodium diatrizoate and polysaccharide

Front 27

uses ammonium chloride

Back 27

erythrocyte lysing agent