NSCLC screening microdevice
Lung cancer has one of the highest incidence and mortality rates among all cancers worldwide. Many cases of lung cancer remain asymptomatic until the disease progresses to an advanced non-curable stage. Currently there are very few early detection techniques. Imaging tests such as CT scans, MRIs, and chest x-rays done on patients in response to other conditions, such as pneumonia or heart disease, can by accident detect lung cancers early in their asymptomatic form. These patients sometimes can respond well to treatment and may even be completely cured of lung cancer. ("Can non-small cell lung cancer be found early?")
Lung cancers are classified either as a non-small cell lung carcinoma (NSCLC) or as a small cell …show more content…
Using either a manual or automatic staining process, the immunoassay will identify the ALK target antigen on freshly prepared and fixed patient sample slides. After the localization of the antigen the antibody-antigen binding site will be made visible under a light microscope by a colored reaction. Methods to visualize will include a tertiary antibody attached to an enzyme that will find and bind to the secondary antibody which is anti-rabbit and bond to the primary. This complex with the corresponding substrate chromogen system results in a red-brown colored precipitate at the site of antibody binding. Additional amplification steps, (Fig.2 steps 4 + 5), may be added to the protocol to increase the intensity of the staining of the rabbit primary antibody to help with visualization. Multimer , followed by a wash, additional DAB and H202 are added to the slide, and finally a reagent containing copper, will amplify the red-brown color to identify the specific antibody-antigen binding sites on the captured CTCs. DAB staining with amplification vs DAB staining without amplification
("CONFIRM™ anti-ALK1 (ALK01) Primary Antibody", 2008)
After staining any presence of strong granular cytoplasmic staining of the captured CTCs will be scored as a positive result. Any staining of cells of the nervous system, glandular epithelial staining, or background stains should be ignored. Absence of strong granular cytoplasmic staining in the tumor cells will consider a negative result. ("CONFIRM™ anti-ALK1 (ALK01) Primary Antibody", 2008)
Tissue positively stained for ALK vs a negative control ("CONFIRM™ anti-ALK1 (ALK01) Primary Antibody",
Lung cancer has one of the highest incidence and mortality rates among all cancers worldwide. Many cases of lung cancer remain asymptomatic until the disease progresses to an advanced non-curable stage. Currently there are very few early detection techniques. Imaging tests such as CT scans, MRIs, and chest x-rays done on patients in response to other conditions, such as pneumonia or heart disease, can by accident detect lung cancers early in their asymptomatic form. These patients sometimes can respond well to treatment and may even be completely cured of lung cancer. ("Can non-small cell lung cancer be found early?")
Lung cancers are classified either as a non-small cell lung carcinoma (NSCLC) or as a small cell …show more content…
Using either a manual or automatic staining process, the immunoassay will identify the ALK target antigen on freshly prepared and fixed patient sample slides. After the localization of the antigen the antibody-antigen binding site will be made visible under a light microscope by a colored reaction. Methods to visualize will include a tertiary antibody attached to an enzyme that will find and bind to the secondary antibody which is anti-rabbit and bond to the primary. This complex with the corresponding substrate chromogen system results in a red-brown colored precipitate at the site of antibody binding. Additional amplification steps, (Fig.2 steps 4 + 5), may be added to the protocol to increase the intensity of the staining of the rabbit primary antibody to help with visualization. Multimer , followed by a wash, additional DAB and H202 are added to the slide, and finally a reagent containing copper, will amplify the red-brown color to identify the specific antibody-antigen binding sites on the captured CTCs. DAB staining with amplification vs DAB staining without amplification
("CONFIRM™ anti-ALK1 (ALK01) Primary Antibody", 2008)
After staining any presence of strong granular cytoplasmic staining of the captured CTCs will be scored as a positive result. Any staining of cells of the nervous system, glandular epithelial staining, or background stains should be ignored. Absence of strong granular cytoplasmic staining in the tumor cells will consider a negative result. ("CONFIRM™ anti-ALK1 (ALK01) Primary Antibody", 2008)
Tissue positively stained for ALK vs a negative control ("CONFIRM™ anti-ALK1 (ALK01) Primary Antibody",