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20 Cards in this Set
- Front
- Back
What are the 5 steps of tissue preparation?
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Fixation, Dehydration & Clearing, Embedding, Sectioning, Mounting & Staining
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What occurs during dehydration and clearing?
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- Replace tissue H2O with ALCOHOL
- Make it transparent with XYLENE |
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What does HEMATOXYLIN stain, and what colour does it stain?
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- Blue
- Nucleus (nucleic ACIDS), acidic parts of cytoplasm, cartilage matrix |
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What does EOSIN stain, and what colour?
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- Pink
- COLLAGEN, basic parts of cytoplasm |
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How does HISTOCHEMISTRY monitor a chemical reaction?
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Monitor formation of insoluble precipitate with a certain colour
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What is the most common histochemical reaction?
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- Schiff (PAS) reagent
- MAGENTA precipitate - Stains glycogen and carbohydrates |
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What is autoradiography?
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Macromolecules labeled with radioactive isotope, visualized with overlay of film
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What is freeze-fracture method used for?
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To visualize macromolecules of INTERNAL aspects of bilayer membranes.
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What is meant by PROTOPLASM?
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Cytoplasm + Karyoplasm -> living substance of cell
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What are ribosomes made of?
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Proteins + mRNA
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Where is SER to be found?
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Cells that make steroids, cholesterol, triglycerides, detox.
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What characterizes the golgi apparatus?
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- 3 faces (cis, medial, trans)
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What characterizes the cis face?
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- Close to nucleus, synthesis of CARBOHYDRATES
- Coatomer PII |
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What characterizes the medial face?
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- Modification of protiens
- Coatomer PI to trans face/export |
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What characterizes the trans face?
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- Sorting of proteins
- Clathrin |
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What differentiates an endosome from a lysosome?
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Endosome has no clathrin or surface receptors - internal vesicle.
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What is Tay-Sachs disease?
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- Little/no HexA is produced
- HexA breaks fatty gangliosides - Gangliosides build and damage nerve cells |
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What are the 2 types of Tay-Sachs?
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1. Classical/infantile; NO HexA
2. Late-Onset (LOTS); lower than normal |
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What are peroxisomes?
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- Self-replicating!
- H2O2, catabolizes long chained fatty assids, detox, killing microorgs |
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Which cells have high amounts of mitochondria?
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- Glandular cells (hormone producing)
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