Hdm Tbl 9 - Humoral Immunity

Front 1

Light Chains have _ _ _ arrangement, and Heavy Chains have _ _ _ _ Arrangement

Back 1

LC: VJC, HC : VDJC

Front 2

CCL19/CCL21 do what?

Back 2

Chemokines, attract immature B-Cells to Lymph Node.

Front 3

_______ attracts B-Cells into Lymph Node Primary Follicle

Back 3

CXCL13

Front 4

Follicular Dendritic Cells produce ____, which provides a signal for immature B-Cells to _______.

Back 4

BAFF -> provides signal for immature (naive) B-Cells to access Follicular Dendritic Cells holding pathogenic antigens.

Front 5

4 Steps of B-Cell Maturation

Back 5

1. CCL19/21 attract immature B-Cellls into Lymph Node.

2. CXCL13 attracts B-Cell into Primary Follicle of Lymph Node.

3. Follicular Dendritic cells and *cytokines* drive B-Cells to mature. BAFF signal causes immature B-Cells to access FDCs and survive.

4. Mature B-Cells exit and circulate in blood/lymph/secondary lymphoid tissues.

Front 6

T-Cell Dependent Pathway is specifically for ________ antigens.

Back 6

Protein

Front 7

What do mature, inactivated B-Cells have?

Back 7

Specificity for a certain antigen.

Front 8

What triggers Mature B-Cell Activation?

Back 8

Antigen presentation within the Primary Follicle to a B-Cell, using a Follicular Dendritic Cell.

Front 9

Step 1 of B-Cell Activation: Dendritic Cells present Antigen inside Primary Follicle to B-Cell and outside Primary follicle to ______.

Back 9

CD4+ Helper T-Cell, triggering differentiation into Tfh Helper T-Cell.

Front 10

Outside of Primary Follicle, a second Dendritic Cell processes an antigen and presents it to a _______. It then secretes _______ and ________ to trigger differentiation of a ______.

Back 10

CD4+ Helper T-Cell

IL-4 and IL-21.

These cytokines trigger Helper T-Cell -> Tfh T-Cell.

Front 11

STEP 2 of B-Cell Activation: Activated B-Cell ______ primary follicle and forms a ________ with _________. It then ______ the primary follicle.

Back 11

Activated B-Cell exits primary follicle, forms a conjugate with Tfh Helper T-Cell, and re-enters the Primary Follicle.

Front 12

STEP 3 of B-Cell Activation: Tfh T-Cell and B-Cell conjugate releases constant ________. This signal is __________ for B-Cell activation, and triggers ________ formation.

Back 12

CD40.

CD40 is co-stimulatory ligand for B-Cell activation.

Triggers Germinal Center Formation.

Front 13

Germinal Center: Also called the _____ _____ _____.

Back 13

Secondary Lymphoid Follicle

Front 14

Germinal Center Mantle Zone: ______ activate _______'s that demonstrate specificity for the original peptide antigen.

Back 14

Tfh T-Cells activate Naive B-Cells that have specific BCRs in the mantle zone, letting them enter the Dark Zone.

Front 15

Germinal Center Dark Zone: B-Cells termed _______ undergo proliferation and __________ __________.

Back 15

B-Cells termed CentroBLASTS undergo proliferation and SOMATIC HYPERMUTATION.

Front 16

Germinal Center Mantle Zone: Full of _____ and ______.

Back 16

Tfh and differentiated B-Cells.

Front 17

Somatic Hypermutation: Tfh secretes ______ which induces ______ to trigger Somatic Hypermutation. This causes ________.

Back 17

Occurs in the DARK ZONE of the Germinal Center

Tfh secretes CD40 cytokine, which induces AID (activation-induced cytidine deaminase) to induce random point mutations in heavy and light chain CDR 1-3, seeking better affinity for the antigen which triggered the Germinal Center to form in the first place.

Front 18

Germinal Center Dark Zone: Affinity Maturation

Back 18

Centroblasts (dark zone B-Cells) that are specific for the antigen of interest undergo Somatic Hypermutation, and only the highest-affinity BCRs survive.

Front 19

CD19, CD21, CD81

Back 19

Co-Receptors for B-Cell Activation, engage antigen upon encountering it in Secondary Lymphoi