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10 Cards in this Set

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binds to and unwinds DNA molecules so that the other proteins can do their job and replicate
DNA helicase
a DNA-bound initiator attracts an enzyme called ___(1), which catalyzes the localized unwinding of the double helix.
(1) DNA helicase
This complex enzyme adds nucleotides, one after the other, to the end of a growing DNA strand...
DNA Polymerase III
Polymerase only operates in the _' -> _' direction.
5' -> 3' direction
find the origin of replication and begin the process of replication during S phase
Initiator proteins
bind single-stranded DNA and help prevent the strands from re-annealing before they are replicated
single-stranded binding proteins
synthesizes an RNA strand to ‘prime’ DNA synthesis. The RNA strand is complementary to the template DNA strand and provides a 3’ end that can be elongated by DNA polymerase.
RNA primase
binds deoxynucleoside triphosphates (dNTPs) and adds them to the RNA primer and the growing DNA chain. DNA polymerase uses the sequence of bases in the template strand to determine which dNTP to add next.
DNA Polymerase
These enzymes function to unravel or untangle DNA strands. They can add or remove positive or negative supercoils.
Topoisomerase/DNA gyrase
joins two DNA strands into one. ____(1) creates a covalent phosphodiester bond between DNA strands by joining the 5’ phosphate from one strand to the 3’ hydroxyl of another strand.
(1) DNA ligase