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55 Cards in this Set

  • Front
  • Back

What are the microorganisms that are of concern in food microbiology?

Bacteria and Fungi (yeast and mold)

Describe how bacteria looks like. Shape? Size?

Spherical, rod-shaped or spiral


1-4 micrometers

What's the dehydrated state of bacteria? Describe how it works.

Spores (endospores). They can survive even under heat, without nutrients, difficult to destroy. When favorable environment comes again, they reactivate.

What's the size and shape of the yeasts?

~20 micrometers, more complex organism, spherical or ellipsoidal

What are yeast's positive function?

Leavening of dough, fermentation of alcohol

What are mold's appearance like?

Larger and more complex than yeasts.


The structure is a multicellular filament called hyphae

When can mold be functional?

Mold-ripened cheese production

What does microorganisms do to food?


Describe in detail

Microorganisms are essentially an enzyme factory: they degrade food systems enzymatically, some even specialize in a substrate they attack: CHO, protein, lipid.

What's the result of the degradation of microorganism on food?

Change in texture,


Off-flavor


Gases, acids, polysaccharide, or alcohol production.

Does microbials prefer high or low moistured environment?

High. At low moisture, they are less able to grow.

Which of the three microorganisms has the least affinity to moisture? (Though they all really love moisture)

Molds

What are the four categories of microbial according to affinity for temperature? What's their favored growing temperature?

mesophilic 20-45


thermophilic >45, some up to 82


psychrophilic <10


psychrotrophic 4: fridge T

What are the two categories of microbial according to need for oxygen?

obligate: strictly aerobic or anaerobic


facultative: survive in either one

What are the two categories of foodborne illnesses? Describe them.

Food infection: Microorganism established itself in intestine and caused the infection


Food intoxication: The toxin produced by the microorganism than causes illnesses.


Some combine both.

Salmonellosis caused by?


Salmonella genus bacteria

Listeriosis caused by?

Listeria monocytogenes

Campylobacteriosis caused by?

Campylobacter genus, primarily C.jejuni

How fast is the onset of food infection and foot intoxication?

Minutes to hours


Days to weeks

What are the three major food-borne infections?

Salmonellosis


Listeriosis


Campulobacteriosis

What's the oxygen requirement state for Clostridium botulinum?

Obligate anaerobe.

What's the acidity affinity for Clostridium botulinum?


What's the line differentiating low-acid and high-acid food?

Likes low acid (more basic) more concern in low-acid canned food.


pH 4.6: Below 4.6, Clostridium cannot reproduce in vegetative state.

What's the reason for botulism to happen?

Clostridium botulinum, producer of botulinum toxin, botox.

What's the occurrence of botulism?


How dangerous?

Very rare


Usually lethal

What's the main vehicle of transmission of Staphylococcal enterotoxins?

Food handlers.

How serious is the intoxication of Staphylococcal enterotoxins?

Not so serious, since only act on intestines.

Where are mycotoxins produced from?

From molds.

What's a hepatotoxin?


Name one

Toxin that damages the liver


Aflatoxin, from Aspergillus flavus.


Liver carcinogens.

What's the most-know enterohemorrhagic toxin- producing strain of the enteral coliform bacteria?

Escherichia coli O157:H7

What are the examples of food intoxication?

Aflatoxin (mycotoxins), Aspergiullus flavus


Staphylococcal enterotoxin, Staphylococcus


Botulinum toxin, Clostridium botulinum

What causes combined infection and intoxication

Escherichia coli O157:H7


(shiga-toxin-producing E.coli: STEC)

What are the risky foods f E.coli O157:H7?

Unpasteurized milk, raw milk cheese, raw meat, etc.

What's the possible outcome disease of STEC infection? Occurrence? Symptoms?

5-10%, hemolytic uremic syndrome. Lysis of RBC,


Kidney failure, permanent kidney damage, death.

How prevalent is STEC?

90 annual death in US. 250,000 illnesses.

How prevalent is norovirus?

Almost half of the outbreak of foodborne illness.

How is norovirus transmitted?

Very easily from person to person. Through food or water

How serious is the norovirus infection?

Not so serious.

Which viruses can be transmitted in food or water?

Hepatitis A, poliovirus, etc.

How prevalent is protozoan parasites diseases?

Death of toxoplasmosis is the second leading cause of death.


From Toxiplasma gondii

What is the surviving environment for T.gondii?

Can survive outside a host in dormant form cysts


or in eggs: oocysts.


How is T.gondii transmitted?

In contaminated food or water.


In warm-blooded animal, esp. cats


In uncooked meat.

How serious is toxoplasmosis?

Serious for weakened immuned people and infants, and transmitted easily in pregnancy.

What are the disease caused by Protozoan parasites?

toxoplasmosis


amoebic dysentery.

What is dysentery?

inflammation of the intestine, causing diarrhea with blood.

Salmonella main source of carrier?

Poultry

How prevalent is salmonellosis?

380 death in US/yr


1 million cases.


Leading cause of death from foodborne illness

Carrier of Listeria?

Chilled food, so "refrigerator disease"


Raw-milk cheese, ready-to-eat meat

How prevalent of Listeriosis?

3rd most prevalent cause of death from food poisoning.

How prevalent is Campylobacteriosis?


How serious?

Most foodborne infections worldwide


Usually not so serious, but long-term effects. for eg. 40% Guillain-Barre caused by campylobacteriosisC

Carrier of Campylobacter?

Poultry

How many E.coli serotypes?


Are they all harmful?


What's the one noted harmful E.coli strain? What does it do?

700 and more


no. microflora in intestines beneficial, but little produce toxins.


shiga-toxin-producing E.coli (STEC), toxin producing, causing RBC lysis

What's the first three most prevalent foodbornecause of death in ?

Salmonellosis


Toxoplasmosis (parasite)


Listeriosis

What's the cause of biggest number of foodborne illness outbreak?

Norovirus

Which pathogenic microorganisms travel in contaminated food and water?

Toxoplasma gondii.


Norovirus

Which pathogenic microorganisms travel in poultry?

Salmonella


Campytobacter

Which pathogenic microorganisms travel in raw milk and raw meat?

Listeriosis


E.coli O157:H7