Chapter 13 Review: Effector Response Cell: Cell And Ab Mediated Immunity

Front 1

4 Functions of Ab

Back 1

Toxin neutralization

Opsonization

Complement fixation

Ab-dependent cell cytotox

Front 2

What Ig Fix complement

Back 2

IgG and IgM

Front 3

What Ig mediated ADCC

Back 3

IgG

Front 4

What is the function of IgE

Back 4

release Inflam. molecules from granulocytes to kill parasites

Front 5

What is the funciton of IgA

Back 5

Major Ig in bodily secretions; block entry of bacteria and toxins to the blood stream

Front 6

What is the role of FcR (3)

Back 6

• Phagocytosis of Ab-Ag complexes by Macrophages
  
• Transcytosis of Ab through epithelial layers
  
• Lysis of Ab-bound NK cells (ADCC)
  

Front 7

T/F ITAM is activating

Back 7

T; ITIM inhibiting

Front 8

3 Effector CElls

Back 8

NK cells - have both innate and adaptive

CD4

CD8

Front 9

Effector cells express higher levels of ____

Back 9

Cell-adhesion molecules

Front 10

T/F Effector cells traffic the same as non effector cells

Back 10

False

Front 11

2 Methods of CTL induced death

Back 11

Fas-FasL

Granzyme

Front 12

Steps of perforin mediated CTL (5)

Back 12

1. TCR-MHC recognition
2. formation of CTL/Target conjugates and immune synapse formation
3. Granule release
4. Dissociation of the CTL from Target
5. Death of target

Fas-FasL is similar

Front 13

T/F NK cells also use Perforin or Fas/FasL

Back 13

T

Front 14

What do NK cells target*

Back 14

Cells that have lose MHC 1 - Ubiq. Self protein

Front 15

2 structural groups of NK cells

Back 15

1) Lectin-like

2) Ig Like

Front 16

Similarities between TC and NKC

Back 16

1. Both express invariant TCR
2. Share common markers
3. Exhibit both Cytotoxic and helper functions
4. Both use Fas-FasL

Front 17

How to measure Cytotox?

Back 17

1) MLR (Mixed Lymphocyte Reaction)

2) CML (cell mediated lympholysis)

{CML uses radioactivity/fluor.}

Front 18

Blocking FCGR3 and CD23 would affect?

Back 18

Opsonization and ADCC - FCGR3

NOT Complement and neutralization - Not FCG dependent

Blocking CD23 has no effect

CD23 is inhib, if anything increases

Front 19

T/F Activation of FcR always leads to internaliztion and destruction

Back 19

F; FCGR2/CD23 is inhib

Front 20

T/F Only CTLs use perforin

Back 20

F; NK cells can as well

Front 21

What R do human NK cells express?

Back 21

KIR

Front 22

What do mitomycin do?

Back 22

blocks proliferation of lymphocytes

Front 23

NOTE: Make sure to determine whether you are looking at PROLIFERATION ASSAYS OR LYSIS CAPABILITY

Front 24

How can you test whether a mouse has Th and Tc cells specific for influenza

Back 24

1. For Tc - CML by incubating spleen from infected mouse ,with mouse with flu-infected syngenic cells
2. For Th - incubate spleen from infected mouse, with APCs; meausure IL-2

Front 25

What signal is stronger in NK cells killing or anti-killing

Back 25

Anti

Front 26

How do NK cells kill

Back 26

They down-regulate MHC 1 which no longer stimulate the INHIB R

Take off the brakes

Front 27

T/F Target cells of Killing express Fas

Back 27

T; Fas L on T cell

Front 28

The stimulation of CD40 on APC by CD40L on Th1 or Th17 leads to what?

Back 28

increased 80/86; licensing

Front 29

TCR activation facilitaes conformational changes in _____ which increases affinity for ______

Back 29

LFA-1

ICAM-1

Front 30

What method is used for Proliferation of T cells?

What method is used for Cytotox. of T cells?

What method is used for T Cell response?

Back 30

Thymadine uptake

Cr release

fluorescent MHC-tetramer

Front 31

What is the role of many complexes of MHC on the fluorescent tool

Back 31

Inreases avidity

Front 32

Steps of MHC-Tetramer

Back 32

1. Attach MHC1 with specific peptide
2. Attach to biotin
3. add to strepavidin (fluorescent)
4. if the MHC1-TCR happens it will fluoresce (use flow)

Front 33

Major cytokine produced by NKC

Back 33

IFN-G

Front 34

What cytokines stimualte by NK cells*?

Back 34

IFN-a, IL-2, IL-12

Happens early

Front 35

What happens when NK cell is presented with Ag in presence of MHC1

Back 35

Inhib reigns supreme and there is no killing

Front 36

Evidence for NK memory*

Back 36

1. NK1.1 is a flow marker for NK cells
   
2. When in contact with infected cell they proliferate
   
3. Repeat infections are fast, efficeint, and long lived
   

Transfer to immunodef. mice provides protection!

Front 37

When we compare survival percentage of NK cells what seem to be true

(Further evidence of memory)

Back 37

10,000 memory had more robust response than 100,000 naive

Front 38

T/F NK cells and CTLs have common markers like CD2, IL-2RB; share common killing pathways, and have similar precursors

Back 38

T

Front 39

Do NK develop in the thymus? NKT?

Back 39

NK - NO

NKT - Yes

Front 40

Do NKT cells have elements of TCR? NK?

Back 40

NKT - YEs

NK - no

Front 41

NKT more like T cell in terms of markers or NK ?

Back 41

NK

Front 42

What presents to NKT?

Back 42

CD1d

Front 43

Activation of FCG leads to what?

Back 43

Activation of cytokine production/ inc. phagocytic activity/lysis by Granuloctyte/MAC/NK

Lyn --> SYK --> effector function