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9 Cards in this Set

  • Front
  • Back

1. Protein Synthesis

A. Transcription


B. one gene at a time


C. three enzymes needed


D. occurs in the nucleus

A. Transcription

1. DNA scribes to mRNA


2. one gene at a time


3. three enzymes needed


4. occurs in the nucleus

B. Translation

1. rRNA translates mRNA using tRNA


2. polypeptide formed from translation


3. amino acid chain assembled on rRNA


4. occurs on the ribosome(rRNA)


5. RER shapes polypeptide into protein

C. Transcription and tanslation

1. is your genotype becoming your phenotype

II. DNA Biotechnology

A. PCR


B. Gene Cloning


C. GMO


D Gene therapy(ex vivo)

A. PCR

1. Polymerase chain reaction


2. copies genes (amplifies them)


3. identifies mutations, daddys and criminals

B. Gene Cloning

1. enzyme cuts out human gene of interest


2. same enzyme cuts vector's genome


3. human gene spliced into vector


4. ligase anneals/link gene to vector's genome


5. vector made to multiple


6. gene is cloned and....


7. in many cases pharmaceuticals are made


(transcription and translation performed)

C. GMO

1. any organism with a foreign gene


2. some processed similar to gene cloning


3. many mass produced foods


4. pest resistant, freeze resistant, more protein, etc

D. Gene Therapy(ex vivo)

1. bone marrow, stem cell removed


2. healthy, correct gene spliced into vector


3. vector usually has retrovirus qualities


4. vector used to "infect" bone marrow stem cells


5. BMSC placed back into patient


6. hopes that new gene synthesizes correct protein