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25 Cards in this Set

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What is the difference between gram positive and gram negative membranes

Gram positive have a thick peptidoglycan layer and no outer lipid membrane

What is lipoteichonic acid?

Teichonic acids that are covalently bound to membrane lipids

Morphology

Refers to the cells shape

Major cell morphologies

Coccus


Rod


Spirillium


Actinobacteria

Gram positive


High G-C content within genome


Largest and most complicated groups of bacteria

Genome makeup

Mycoplasma

Tenericutes - gram positive


Some of the smallest organisms capable of autonomous growth


Lack cell walls

Pleomorphic

Groups of cells of the same species are very different from each other

Lactobacillus

Firmicutes


Fermentative bacteria that produces lactic acid from lactose


Used within the food industry extensively

Streptococcus

Gram positive


Important for the production of buttermilk, silage and other products

Staphylococcus

Commonly found in humans and animals


S.aureus is associated with many pathological conditions

Bacillus, Clostridium and sporosarcina

Gram positive bacteria with low GC content

What are the three forms of anthrax

Cutaneous


Gastrointestinal


Inhalational

AB toxins

Toxins with an intracellular target that have two functionally distinct region


A has enzymatic activity (part that causes the damage)


B is responsible for host cell binding or entry of A unit into cell

What is unusual about anthrax toxin

It has three toxin subunits


2A unit and 1B unit

Extracellular components of gram negative bacteria

Capsule


Fimbriae


Flagella


Pilus

Fimbrae

Extracellular component of gram negative bacteria that allows attachment to surfaces

Pilus

Extracellular component that allows gram negative bacteria to share genetic info

Proteobacteria is sub-divided into 5 classes

Alpha


Beta


Delta


Gamma


Epsilon


Gammaproteobacteria contains many pathogen organisms such as

Escherichia


Salmonella


Shigella


Klebsiella

Ecoli morphology

Straight rods

Pseudomonad aeruginosa

Gammaproteobacteria that causes cystic fibrosis

Two ways to isolate microbes from the environment

Direct isolation


Enrichment culture

How is a most probable number calculated

Serial dilution is carried out until final tube shows no growth

Copiotrophs

Microbes that exist primarily in a resting phase with brief periods of activity

Why would a bacteria be viable but nonculturable

Bacteria is in a state of very low metabolic activity


They do not divide