Blood Smear View Book

Front 1

This change seen in what conditions (5)?

Back 1

spherocytes

Seen in:
Warm AIHA
Old blood
Burns
Venoms
Oxidant damage

Front 2

Seen in what conditions? (2)

Back 2

Eliptocytes

Conditions:
Hereditary ellipotcytosis
Iron deficiency

Front 3

Seen in what conditions? (4)

Back 3

Echinocytes

Conditions:
ARF
prolonged EDTA
Low pH slides
Pyruvate kinase deficiency, post splenectomy

Front 4

Seen in what conditions? (6)

Back 4

Acanthocytes

Conditions:
Liver failure
a-beta-liproporteinemia
hypothyroid
post-splenectomy
McLeod (no Kx)
MDS

Front 5

Seen in what conditions? (4)

Back 5

Target cells

Conditions:
Liver failure
Hemoglobinopathies
Asplenia
Iron deficiency

Front 6

What condition? (1)

Back 6

Bite cells

Condition:
G6PD deficiency (munching of Heinz body), may be accompanied by blister cells

Front 7

Causes? (7)

Back 7

Basophilic stippling (ribosomal RNA)

Causes:
Lead poisoning
Hemoglobinopathy
Pyrimidine 5' nucleotidase deficiency
MDS
Infection
Sideroblastic anemia
Porphyria

Front 8

causes? (3)

Back 8

Howell Jolly body (nuclear remnant)

Causes:
Post-splenectomy/asplenia
Megaloblastic anemia
MDS

Front 9

Causes (2)?

Back 9

Pappenheimer body (mitochondrial remnants)

Causes:
post-splenectomy/asplenia
Fe overload

Front 10

Causes (2)?

Back 10

Cabot ring: microtubule, remnants of the mitotic spindle

Causes:
Disordered erythropoiesis
Severe megalobastic anemia

Front 11

Crystal violet stain. What condition?

Back 11

Heinz body

Conditions:
G6PD deficiency

Front 12

Back 12

Iron deficiency anemia, post transfusion. The cells are pale with an enlarged central pallor, in sharp contrast to the transfused normochromic cells.

Front 13

Back 13

Blood film, megaloblastic anemia. Macrocytosis and a circulating megaloblast with abnormal, binucleated nucleus and open chromatin.

Front 14

Back 14

Sickle cell preparation, in sodium metabisulfate; sickle cell anemia. Even in sickle cell trait, all cells eventually sickle.

Front 15

Back 15

Blood film, sickle cell anemia. The erythrocytes are far from one another, suggesting a severe anemia. Numerous pointed sickle cells and target cells are present. Close to the nucleated red blood cell, elliptical cells are seen. These cells have a dense center, instead of the usual central pallor, suggesting that they are incipient sickle cells (500?).

Front 16

Back 16

Hemoglobin (Hb) C disease, after splenectomy. Before splenectomy, the only morphologic abnormality was the presence of target cells. After splenectomy, Howell-Jolly bodies and Hb crystals, such as those in the center, were present. Note that almost all of the Hb in this particular cell is in the dark bar, and the membrane is still visible. Some such crystals are distinctly hexagonal (1000?).

Front 17

Back 17

Homozygous β-thalassemia. A few cells contain hardly any hemoglobin (Hb), and the Hb is often precipitated at the membrane. Bizarre target cells, Howell-Jolly bodies, and poorly hemoglobinized nucleated red blood cells are seen.

Front 18

Back 18

β-Thalassemia trait. Hypochromic, microcytic red blood cells with frequent targeting. Mild anemia.

Front 19

Back 19

Hemoglobin H disease. Mild anemia and target cells.

Front 20

Back 20

Hemoglobin H preparation. Film made after incubation of blood with brilliant cresyl blue. Several red cells contain multiple small pale blue inclusions.

Front 21

Back 21

Heinz bodies. Normal red cells incubated with an oxidant drug and stained while in suspension with methyl violet; finally, an air-dried film was made and stained with Wright's stain. Purple-staining Heinz bodies are precipitates of denatured hemoglobin that tend to attach to the cell membrane.