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23 Cards in this Set
- Front
- Back
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what is the chemical used to fix proteins, prevent autolysis and decomposition?
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Formaldehyde
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What is used to cut paraffin sections which are hundreds of microns? (Rotary microtome/vibratome/cryostat/ultramicrotome)
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Rotary microtome
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What is used to cut live/fixed brain tissue which are not embedded? (Rotary microtome/vibratome/cryostat/ultramicrotome)
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Vibratome
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What is used to cut frozen brain (fixed blade and cuts tissue sliced off slowly) (Rotary microtome/vibratome/cryostat/ultramicrotome)
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Cryostat
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What is used to section embedded in epoxy resin - a diamond knife semi thin sections 1 ultrathin section (Rotary microtome/vibratome/cryostat/ultramicrotome)
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Ultramicrotome
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What is the stain used to detect DNA in living and fixed neurones? (DAPI/Nissl/Golgi/Nauta silver stain)
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DAPI
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What is the stain used to detect neurone and glia in the brain? (DAPI/Nissl/Golgi/Nauta silver stain)
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Nissl
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What is the stain used to detect neurones in the brain? (DAPI/Nissl/Golgi/Nauta silver stain)
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Golgi
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What is the stain used to detect degenerating axon? (DAPI/Nissl/Golgi/Nauta silver stain)
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Nauta silver stain
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Nissl stain selectively labels neurons and gives an indication of the __________________ of neurons in different parts of the brain
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cytoarchetecture
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The disadvantage of using a nissl stain is that it only labels neurons including and surrounding the nucleus. What is not labelled?
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Neutrites and axon terminals
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The advantage of the golgi stain is that it stains both the ________ and the _______ of the neurons
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cell body
neuritis |
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Golgi suggested that neurites of different cells were fused together to form a _______ ___________
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continuous reticulumn
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Cajal neurone doctrine argued that nuerons were like other cells in the body and were the ________ _____ of the brain
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elementary unit
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What is the limit of a light microscope?
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0.1micrometers
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What is the space between the neurons?
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0.02micrometers
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What is the resolution of electron microscope?
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o.1nanometer
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The electron microscope revealed the exsistence of synapses and the ultrastructure such as
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the mitochondria
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What is immunohistochemistry?
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The manufacture of antibodies to the candidate protein to visualise the effect on the section of the brain tissue and their production of protein
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Fluoresence histochemistry reveals ______ neurons in the brain
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dopaminergic
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Neurons uses ________ to transport substances from the cell body to the axon terminal
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microtubules
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What is the term used for the movement of substances from the soma to the axon terminals?
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anterograde transport
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What is the term used for the movement of substances from the axon terminals to the soma?
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retrograde transport
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