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96 Cards in this Set

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-pertain to the multiplication of cells rather than the size of cell


- growing microbes accumulates into colonies

Microbial growth

Growth reqts- Physical



Temperature


Extreme cold

Pschrophiles

Growth reqts- PhysicalTemperature Room temperature

Mesophiles

Growth reqts- PhysicalTemperature Extreme heat

Thermophiles


(Hyperthermophiles or extreme thermophiles)

Growth reqts- Physical Temperature Can grow well in refrigirator temperature

Pychotrophs

Growth reqts- Physical


Lowest temperature that the species can growth

Minimum growth temperature

Growth reqts- Physical



Best temperature for growth

Optimum

Growth reqts- Physical



Highest temperature possible for growth

Maximum

Growth reqts- Physical



PH


Most bacteria growth at pH _?_ to __?_

pH 6.5 to pH 7.5

Growth reqts- Physical



pH ??????



Responsible for preserved fermented food


Ex.. Pickles and cheese

pH 4

Growth reqts- Physical



pH


Give the 3 bacteria grow at pH 5 to 6

Mold, fungi and yeast

Tolerant to acidity

Acidophiles

Growth reqts- Physical



Importance for the maintenance of the medium

Osmotic pressure

Growth reqts- Physical



Osmotic loss of water, shinkage of the cell's cytoplasm

Plasmolysis

Growth reqts- physical



Requires high salt concentration for growth

Extreme halophiles o obligate halophiles

Growth reqts- physical



Do not require salt but can growth at 12-15℅ salt concentration

Facultative halophiles

Growth reqts- chemical



Obtain this from proteins, lipids and carbohydrates

Chemoheterotrophs

Growth reqts- chemical



From carbon dioxide

Chemoheterotrophs and photoautotrophs

Growth reqts- chemical


For the synthesis of cell structure



Nitrogen and sulfur

Protein synthesis

Growth reqts- chemical For the synthesis of cell structure



Nitrogen and phosphorus


ATP, DNA, and RNA

Growth reqts- chemical For the synthesis of cell structure



Sulfur

Sulfur containing amino, thiamine, biotin

Growth reqts- chemicalFor the synthesis of cell structure



Phosphorus

Nucleic acid and phospholipid

Growth reqts- chemical



Give some example of trace element.


Functions as confactor

Small amount of iron, copper, molebdenum, and zinc

Growth reqts- chemical



Require oxygen to live

Obligates aerobes

Growth reqts- chemical



Utilize oxygen to growth but can still survive in the absence of oxygen

Facultative anaerobes

Growth reqts- chemical



Unable to survive in the presence of oxygen

Obligate anaerobes

Growth reqts- chemical



Cannot use oxygen for growth but can tolerate it

Aerotolerant anaerobes

Growth reqts- chemical



Aerobic but do not require oxygen to grow in low concentrations of oxygen

Microaerophiles

Growth reqts- chemical



Grow in high CO2 concentration

Capnophiles

Growth reqts- chemical


Peroxide


__?____- convert water to oxygen

Catalase

Growth reqts- chemical



Peroxide


___?___- convert it to oxygen

Peroxidase

Nutrient materials prepared from the lab for the grow of microorganism



Ex: nutrient agar

Culture medium

Microbes that are introduced into a culture medium to initiate gowth

Inoculum

Microbes that grow and multiply in a culture medium

Culture

@ complex polysaccharide derived fro marine alga (japanese isinglas- gelidium cartilagineum)



@ used as thickener

Agar

For organisms requiring high protein diet

Milk agar

For organism requiring hemoglobin




( hemoglobin require this organism)


( joevelyn require this to eat)

Chocolate agar

Determine the abilty of an organism to ferment lactose

Mac conkey agar

Same use as that of Mac Conkey, differentiates of coliform bacteria

Eosin methylene blue (EMB)

Identification of species that destroy RBC (streptococcus)

Blood agar plate (BAP)

Selective for gram (-) so will allow gram (+) to grow

Colestin nalidixic acid (CNA) agar

Selective for fungi pH 5.6

Sabouraud dextrose agar

Selective media



Modified chocolate agar w/c selective for neisseria gonorrhoeae

Thayer- Martin Agar

4 phases of bacterial growth

Lag phase


Log phase aka exponential phase


Stationary phase


Death phase aka death phase

Proteobacteria- largest group of bacteria


- from the greek god proteus



Give 5 classes of proteobacteria?

@Alphaproteobacteria- agriculturally important bacteria


@Betaproteobacteria


@Gamaproteobacteria


@Deltaproteobacteria- important conributors for sulfur cycle


@epsilonproteobacteria

Most abundant organism in the ocean

Pelagibacter ubique

Causing ehrlichiosis


Manifested flu like

Ehrlichia

ID test for agglutination of rickettsia infection

Well felix test

Epidemic typus

Rickettsia prowazekii

Endemic typus

Rickettsia typhi

Rocky mountain spotted fever

Rickettsia ricketsii

Causes cat- scratch disease

Bartonella henselae

Causes brucellosis


Aka undulant fever

Brucella

Causes brucellosis


Aka undulant fever

Brucella

Most common infectious bacteria in the world

Wolbachia

Sulfur - oxidizing bacteria

Thiobacillus

Usually used as a demo slide

Spirillum volutans

Cause whooping cough

Bordetella pertussi

Gonococcal gonnorhea

Neisseria gonorrhoeae

Meningococcal meningitis

Neisseria meningitidis

Causes tularemia

Francisella tularensis

Causes conjunctivitis

Moraxella lacunata

Causes legionellosis

Legionellales burnetti

Causes Q fever

Coxiella burneti

Causative agent for cholera




Manifestation ; rise water stool

Vibrio cholerae

Transmission by raw or undercooked shellfish

Vibrio parahemolyticus

Most common inhabitant of human intestinal tract



Usually non- pathogenic

Escherichia coli

System to differentiate the serovars

Kauffman- white scheme

Substance obtained from the heme faction needed for respiration

X factor

Cofactor

V factor

Causes abortion in domestic animals

Campylobacter fetus

Causes foodborne intestinal disease



Culture in skirrow's agar

Campylobacter jejuni

Causes peptic ulcer

Helicobacter

Nice to know...

Tetanus

Clostridium tetani

Botulism

Clostridium botulinum

Gas gangrene

Clostridium perfringes

Causes serious diarrhea; enterocolitis

Clostridium dificile


DOC metronodazole


Cigar shaped discovered in the red sea



Name means


"Guest at the banquet of a fish"

Epulospicium

Anthrax

Bacillus anthracis

Best known microbial insect pathogen

Bacillus thuringiensis

Causes of food poisoning in stachy food

Bacillus cereus

Cause toxic shock syndrome

Staphylococcus

Scarlet fever

Streptococcus pyogenes

Neonatal sepsis

Streptococcus agalactiae

Pneumococcal pneumonia

Streptococcus pnemoniae

Dental caries

Streptococcus mutans

Found in large number in human tool

Enterococcus

Contaminate daily product



Manifestation listeriosis

Listeria

Diptheria ( grayish membrane)

Corynebacteria diphtheria

Primary causes of acne

Propionibacterium acne

Causs the most common form of vaginitis

Gardnerella

Syphilis

Treponema palladium

Lyme disease; relapsing fever

Borrelia burdorferi

Leptospirosis

Lestospira interrogana

Growth reqts- chemical



Normal molecular oxygen that has been boosted, thus highly reactive

Singlet oxygen