Assignment #2: The Replication of DNA in Escherichia Coli
Due Date: Feb 8, 2016 at 9:00 AM CST
1a. A procedure in which a small amount of DNA material is immersed in concentrated solution, cesium chloride (CsCl), and soon after placed in centrifuge until the point where equilibrium is achieved is called density-gradient centrifugation. Contrasting techniques involving diffusion and sedimentation form a persisting CsCl concentration gradient. Furthermore, the concentration gradient and pressure of centrifugal force results in increased density. And when solution density corresponds to the buoyant density, DNA reaches a more balanced state.
1b. The generation time refers to the length required for a single cell to split apart into two separate cells. …show more content…
Isotopes are elements that have the same number of protons, but differ in the number of neutrons. Also, isotopes demonstrate the same chemical properties, but vary in atomic mass.
2a. The density difference between the initial DNA (at zero generation) and the DNA after one generation is 0.007 gm. cm-3. Between DNA N14 and N15, the density difference is seen being halved; this is indicated as a half-labeled “hybrid” molecule. Attributed to the results, it is possible for the conservative-based model of DNA replication to be removed.
2b. The DNA density distribution is positioned at the midpoint in the midst of the N14 and N15 DNA density after one generation. Compared to after 2.5 generations, the DNA density distribution lies mainly towards the N14 DNA density, where a small amount remains situated at the midpoint. Attributed to the results, it is possible for the dispersive-based model of DNA replication to be removed.
2c. Besides 0, 0.3, and 0.7, there are no other generations where the high-density DNA exists.
3a. The nitrogenous base is the component of a DNA nucleotide which contains
Due Date: Feb 8, 2016 at 9:00 AM CST
1a. A procedure in which a small amount of DNA material is immersed in concentrated solution, cesium chloride (CsCl), and soon after placed in centrifuge until the point where equilibrium is achieved is called density-gradient centrifugation. Contrasting techniques involving diffusion and sedimentation form a persisting CsCl concentration gradient. Furthermore, the concentration gradient and pressure of centrifugal force results in increased density. And when solution density corresponds to the buoyant density, DNA reaches a more balanced state.
1b. The generation time refers to the length required for a single cell to split apart into two separate cells. …show more content…
Isotopes are elements that have the same number of protons, but differ in the number of neutrons. Also, isotopes demonstrate the same chemical properties, but vary in atomic mass.
2a. The density difference between the initial DNA (at zero generation) and the DNA after one generation is 0.007 gm. cm-3. Between DNA N14 and N15, the density difference is seen being halved; this is indicated as a half-labeled “hybrid” molecule. Attributed to the results, it is possible for the conservative-based model of DNA replication to be removed.
2b. The DNA density distribution is positioned at the midpoint in the midst of the N14 and N15 DNA density after one generation. Compared to after 2.5 generations, the DNA density distribution lies mainly towards the N14 DNA density, where a small amount remains situated at the midpoint. Attributed to the results, it is possible for the dispersive-based model of DNA replication to be removed.
2c. Besides 0, 0.3, and 0.7, there are no other generations where the high-density DNA exists.
3a. The nitrogenous base is the component of a DNA nucleotide which contains