Plasmid

Addition of the Plasmid vs the Expression of the Added Plasmid Introduction The earliest example of genetic engineering was performed by Gregor Mendel in 1865, when Mendel performed artificial selection on a population of pea plants in the monastery where he lived (http://www.sciencegroup.org.uk/ifgene/history.htm). Once the technological advancements of the 20th century was introduced to the science field, genetic engineering took on a whole new light. Scientist could now transform organisms in ways that no one ever could. E. coli is the perfect test subject for this lab because, as stated by Pearson schools, “E. coli reproduce very rapidly; a single microscopic cell can divide to form a visible colony with millions of cells overnight. Like…

To start this experiment, two microcentrifuge tubes were exposed to different ingredients. One was labeled “+DNA,” the other with “-DNA.” They were both filled with different bacteria. More specifically, the “+DNA” tube had pGFP in it and included a plasmid. Included on that plasmid were two genes: gfp (gene for green fluorescent protein) and an ampicillin resistant gene. The gfp gene makes cells glow and the resistant gene fights against ampicillin, which kills cells. This plasmid DNA was…

Introduction Plasmids, circular pieces of DNA that can replicate independently from the host cell’s DNA, have been extensively used to transform cells in biological research to study the effects of particular genes of interest (Lau et al., 2013). A plasmid’s basic components include a replication origin, a DNA marker, and a multiple cloning site, which are sufficient for the plasmid to replicate itself and help the transformed cell to exhibit characteristics indicating successful…

Abstract Plasmids are circular clusters of DNA that carry valuable genes that help a host survive. The process of transformation can occur and the genetic material of the cell can be altered with a plasmid. However, the cell must be competent so it has the ability to take in DNA from the environment in order for transformation to occur properly. The E.Coli was treated with CaCl2 in order for the cell to be competent. The presence or absence of ampicillin is used to test the E.Coli’s competence…

coli, have their DNA in the form of a plasmid. A plasmid is a circular ring of DNA. The plasmid intended to transform the E. coli colonies for the purposes of this study was the pGLO plasmid. The pGLO plasmid contains three main genes: one for GFP (green fluorescent protein), one for ampicillin (an antibiotic) resistance, and one that codes for the sugar arabinose (Bassiri 2011). The process used to transform the E. coli bacteria with the pGLO plasmid was by means…

limited time constraints and having lab only once a week this lab was broken up into five periods, the last one specifically to go over the results. Day one consisted of extraction of plasmid DNA, which we used Resuspension Buffer to break up our pellet after removing the supernatant (liquid) after centrifuging our overnight culture. Then Lysis Buffer was used to break down the membrane of the cells so that we could then add Neutralization Buffer to bring our sample from basic to neutral pH.…

A plasmid is circular, relatively small in size, double stranded as a DNA molecule, and is physically distinct from the chromosomal DNA of a cell. Innately found in prokaryotic bacterial cells, plasmids can sometimes be found in eukaryotes as well 1. Plasmids are self-replicating; therefore, when a plasmid is formed, infinite copies of the plasmid can be created if the plasmid is grown in bacteria. 2 Because of the size of plasmids, which can range anywhere from one thousand base pairs up to…

Introduction: In this lab experimentation, the group conducted Deoxyribonucleic acid isolation and restriction analysis on a plasmid from Escherichia coli cells. Plasmids are small circular DNA that are in the bacterium cells. Escherichia coli is a gram- negative bacterium that is known for variable reaction to antibiotics, and can be genetically manipulated. The gram- negative bacterium, Escherichia coli can be genetically manipulated by extracting a certain plasmid that allows it to resist…

bacterium cell walls more permeable and a heat shocking method to introduce the pGLO plasmid in the E.coli bacterium so that they may exhibit ampicillin resistance. The Goal of the experiment was to observe whether or not, given one of the four specific conditions, the pGLO plasmid would be able to transcribe itself into the E.coli bacterium. If the cells were successful in up-taking the plasmid, then they would inherently become resistant to ampicillin, be able to grow into colonies, and…

and F′lac+ donors Abstract The process in which DNA from a donor cell is transferred through physical contact into a recipient cell is known as conjugation. In the experiment, sexduction, a cross between F′ and F- plasmids, was demonstrated by mixing the donor and recipient, diluting them and using selective plating methods. The plasmids from the donor, recipient and transconjugant were isolated and analyzed using FIGE gel Electrophoresis. The data that was acquired consisted of 12 red…