Separation of plasma proteins by molecular exclusion chromatography
Sebastian Prisacariu
140013826
Introduction
The components of blood is broken down into 3 parts. This breakdown is consisted of: Plasma which is a yellowish fluid that helps transport blood cells around the body and contains a small supply off proteins, platelets which are blood clotting agent, red blood cells which is a carry for oxygen and remover off carbon dioxide and white cells which is the bodies defence from infection. The plasma makes up about 55% of the overall volume of blood and is mainly made up of water which contains important proteins like albumin which maintains fluid balance (Nall, 2013) and globulin which serves as antibodies and helps with transport (Clinn, …show more content…
When comparing this result to those found in Figure 1 from the elution of the blood serum, the profile shown for Iron (III) Chloride is what I would expect. The peak off the elution profile is fraction number 3 which falls in between the highest fraction number of protein which is 2 and the highest fraction number of sodium ions which is 5 according to Figure 1. This can be justified by looking at the molecular weight off all three. The molecular weight of Iron (III) chloride is 162.20 g/mol (Anon, 2002) which is above that of a single sodium ion which is 22 g/mol (Burrows et al., 2013) and below the molecular weight of either of the proteins found in the blood serum, Albumin and Globulin (WebMD, 2014). This in conclusion justifies Iron (III) Chloride position in the elution …show more content…
and Price, G. (2013). Chemistry³. Oxford: Oxford University Press, p.22
Clinn, S. (1990). Sex hormone binding globulin: origin, function and clinical significance. - PubMed - NCBI. [online] Ncbi.nlm.nih.gov. Available at: http://www.ncbi.nlm.nih.gov/pubmed/2080856 [Accessed 8 Oct. 2015].
Discoveriesinmedicine.com, (2006). Chromatography - used, first, blood, body, plant, uses, history, The First Chromatograph, Ion-Exchange Chromatography, Paper chromatography, Martin and Synge, Gas chromatography. [online] Available at: http://www.discoveriesinmedicine.com/Bar-Cod/Chromatography.html [Accessed 28 Sep. 2015].
Healthcare, G.E. (2013) ‘SephadexTM G-25 media and prepacked formats’, GE Healthcare Bio-Sciences AB, Data file , pp. 1–11.
Nall, R. (2013). Role of Albumin | LIVESTRONG.COM. [online] LIVESTRONG.COM. Available at: http://www.livestrong.com/article/488109-role-of-albumin/ [Accessed 8 Oct. 2015].
Not, D.O. and Vacutainer, B.D. (2009) ‘Centrifuge tubes Cryovials Vacutainer tubes ( containing either no additive or a clot activator ) No additive : e . g . BD Vacutainer Plus tube with clear BD Hemogard closure , 3 ml , clear top (# 366703 ) Centrifuge tubes Cryovials Benchtop centrifuge ( ’, Blood, 44(0), pp.
Sebastian Prisacariu
140013826
Introduction
The components of blood is broken down into 3 parts. This breakdown is consisted of: Plasma which is a yellowish fluid that helps transport blood cells around the body and contains a small supply off proteins, platelets which are blood clotting agent, red blood cells which is a carry for oxygen and remover off carbon dioxide and white cells which is the bodies defence from infection. The plasma makes up about 55% of the overall volume of blood and is mainly made up of water which contains important proteins like albumin which maintains fluid balance (Nall, 2013) and globulin which serves as antibodies and helps with transport (Clinn, …show more content…
When comparing this result to those found in Figure 1 from the elution of the blood serum, the profile shown for Iron (III) Chloride is what I would expect. The peak off the elution profile is fraction number 3 which falls in between the highest fraction number of protein which is 2 and the highest fraction number of sodium ions which is 5 according to Figure 1. This can be justified by looking at the molecular weight off all three. The molecular weight of Iron (III) chloride is 162.20 g/mol (Anon, 2002) which is above that of a single sodium ion which is 22 g/mol (Burrows et al., 2013) and below the molecular weight of either of the proteins found in the blood serum, Albumin and Globulin (WebMD, 2014). This in conclusion justifies Iron (III) Chloride position in the elution …show more content…
and Price, G. (2013). Chemistry³. Oxford: Oxford University Press, p.22
Clinn, S. (1990). Sex hormone binding globulin: origin, function and clinical significance. - PubMed - NCBI. [online] Ncbi.nlm.nih.gov. Available at: http://www.ncbi.nlm.nih.gov/pubmed/2080856 [Accessed 8 Oct. 2015].
Discoveriesinmedicine.com, (2006). Chromatography - used, first, blood, body, plant, uses, history, The First Chromatograph, Ion-Exchange Chromatography, Paper chromatography, Martin and Synge, Gas chromatography. [online] Available at: http://www.discoveriesinmedicine.com/Bar-Cod/Chromatography.html [Accessed 28 Sep. 2015].
Healthcare, G.E. (2013) ‘SephadexTM G-25 media and prepacked formats’, GE Healthcare Bio-Sciences AB, Data file , pp. 1–11.
Nall, R. (2013). Role of Albumin | LIVESTRONG.COM. [online] LIVESTRONG.COM. Available at: http://www.livestrong.com/article/488109-role-of-albumin/ [Accessed 8 Oct. 2015].
Not, D.O. and Vacutainer, B.D. (2009) ‘Centrifuge tubes Cryovials Vacutainer tubes ( containing either no additive or a clot activator ) No additive : e . g . BD Vacutainer Plus tube with clear BD Hemogard closure , 3 ml , clear top (# 366703 ) Centrifuge tubes Cryovials Benchtop centrifuge ( ’, Blood, 44(0), pp.