Introduction:
In many cases in industry, it is necessary to separate a mixture or solution. In environmental testing, they look for PCB’s in oil, and pesticides in ground water. In the food industry, they can test additives to determine concentrations of preservatives, vitamins, proteins, and amino acids.2 Chromatography is the process of separating a mixture, and has many different applications throughout the world. There are a variety of ways to conduct chromatography including column chromatography, paper chromatography, and thin layer chromatography. The particular experiment conducted and discussed is paper chromatography. This experiment will use chromatography to test and identify unknown pen inks based on tests done on known pen inks.
Understanding the history of chromatography can help us understand different uses of the technique. A Russian botanist invented chromatography to separate and distinguish plant pigment. Mikhail Semenovich Tsvett trickled a mixture of dissolved pigments through a glass tube filled with a tight calcium carbonate …show more content…
Other methods, such as laser desorption, anionic dyeing, and capillary electrophoresis are used in forensics, however for this experiment paper chromatography worked well because of its simplicity, low cost, and ability to be adjusted. Throughout this lab, the group will test various solutions to conduct paper chromatography with, and then will collectively decide on the best test method. Initially, all group members tested a standard 2:1 1-proponal to water solution, with a polarity of 5.81. The group then tested four different solutions as described in the procedure, expecting the 2:1 1-proponal to ethanol solution to have the best chromatogram based on polarity. The group had decided before the experiment that a lower polarity in the mobile phase solution would not take the pen mark as far, and thus giving us a tighter
In many cases in industry, it is necessary to separate a mixture or solution. In environmental testing, they look for PCB’s in oil, and pesticides in ground water. In the food industry, they can test additives to determine concentrations of preservatives, vitamins, proteins, and amino acids.2 Chromatography is the process of separating a mixture, and has many different applications throughout the world. There are a variety of ways to conduct chromatography including column chromatography, paper chromatography, and thin layer chromatography. The particular experiment conducted and discussed is paper chromatography. This experiment will use chromatography to test and identify unknown pen inks based on tests done on known pen inks.
Understanding the history of chromatography can help us understand different uses of the technique. A Russian botanist invented chromatography to separate and distinguish plant pigment. Mikhail Semenovich Tsvett trickled a mixture of dissolved pigments through a glass tube filled with a tight calcium carbonate …show more content…
Other methods, such as laser desorption, anionic dyeing, and capillary electrophoresis are used in forensics, however for this experiment paper chromatography worked well because of its simplicity, low cost, and ability to be adjusted. Throughout this lab, the group will test various solutions to conduct paper chromatography with, and then will collectively decide on the best test method. Initially, all group members tested a standard 2:1 1-proponal to water solution, with a polarity of 5.81. The group then tested four different solutions as described in the procedure, expecting the 2:1 1-proponal to ethanol solution to have the best chromatogram based on polarity. The group had decided before the experiment that a lower polarity in the mobile phase solution would not take the pen mark as far, and thus giving us a tighter