Calculations-
Representative Fraction (RF): 0.92cm / 8.95cm = 0.10
Limitations-
Ruler: +/- 0.05 cm
One possible limitation for this experiment was the contamination of the chromatography paper by foreign amino acids. Any amino acids that our gloves came in contact with throughout the lab transfered over to the chromatography paper and appeared as purple spots, mostly on the border of it.
Conclusion-
The amino acids in unknown mixture A were Glycine and Valine. The amino acids in unknown mixture B were Glycine and Leucine. The amino acids in unknown mixture C were Lysine and Prolise.
Questions-
The markings from a lead/graphite pencil will not enter the mobile phase so it will remain stationary while the ink from a pen will enter the
Representative Fraction (RF): 0.92cm / 8.95cm = 0.10
Limitations-
Ruler: +/- 0.05 cm
One possible limitation for this experiment was the contamination of the chromatography paper by foreign amino acids. Any amino acids that our gloves came in contact with throughout the lab transfered over to the chromatography paper and appeared as purple spots, mostly on the border of it.
Conclusion-
The amino acids in unknown mixture A were Glycine and Valine. The amino acids in unknown mixture B were Glycine and Leucine. The amino acids in unknown mixture C were Lysine and Prolise.
Questions-
The markings from a lead/graphite pencil will not enter the mobile phase so it will remain stationary while the ink from a pen will enter the