DISCUSSION
6.1 FTIR SPECTROSCOPY
It was observed from the FTIR graph that there was no shifting of peaks of metronidazole i,e., characteristic peak of the amine group (N-H streching) present in imidazole nucleus at 1030 cm-1 and presence of NH2 group at 3298 cm-1 in formulation. Hence, there was no interaction between drug and polymer.
6.2 METRONIDAZOLE MICROSPHERES
6.2.1 Optimization of speed (rpm) for preparation of microspheres
Metronidazole microspheres were prepared using emulsion solvent evaporation technique. For optimization of stirring speed, the microspheres were prepared at various speed such as at 1250, 1500, 1750 and 2000 rpm. Out of these, at 1500 rpm gave the formation of well formed microspheres with maximum encapsulation …show more content…
Vulcanization of rubber creates more disulfide bonds between chains, so that the chains tighten and makes rubber harder and less extensible.
The tensile strength of the various batches of natural rubber latex intravaginal ring containing drug in various concentration was estimated. The addition of drug in intravaginal ring used is inversely proportional to the tensile strength i.e., IVR containing 190 mg, 210mg drug and microsphere containing 190 mg of drug shows 0.438 KgF/cm2, 0.419 KgF/cm2, 0.309 KgF/cm2 and 0.218 KgF/cm2 of tensile strength respectively. This is due to addition of drug in IVR leads to reduction of natural rubber latex volume causing less disulfide bond formation.
B] Folding …show more content…
Hence 5 h time and 1000C temperature optimized for formation of further IVR batches. The folding endurance of the various batches of natural rubber latex intravaginal ring containing drug in various concentration was estimated. The addition of drug in intravaginal ring used is inversely proportional to the folding endurance i.e., IVR containing 190 mg, 210 mg drug and microsphere containing 190 mg of drug shows 336, 310, 241 and 174 folding endurance respectively. This is happened due to addition of drug in IVR leads to reduction of natural rubber latex volume in the ring causing less availability of rubber latex for disulfide bond formation.
6.3.2 In vitro drug release
The drug release from batch R2 i.e. IVR containing 210 mg drug, showed more release (42.17%) in 12 h as compared to other batches (Table 22). Subsequently, the slow release was obtained due to the leaching action. These rings showed a large initial burst of drug release in 12 h which slowly decreases over 24 h and to 120 h. The 12 h burst, representing release of the metronidazole initially present at the surface of the ring. During vulcanization insoluble film of rubber entrap the suspended drug particles, this his leads to decrease in penetration of dissolution fluid and leaching out of drug resulting in slow drug release. The drug release
6.1 FTIR SPECTROSCOPY
It was observed from the FTIR graph that there was no shifting of peaks of metronidazole i,e., characteristic peak of the amine group (N-H streching) present in imidazole nucleus at 1030 cm-1 and presence of NH2 group at 3298 cm-1 in formulation. Hence, there was no interaction between drug and polymer.
6.2 METRONIDAZOLE MICROSPHERES
6.2.1 Optimization of speed (rpm) for preparation of microspheres
Metronidazole microspheres were prepared using emulsion solvent evaporation technique. For optimization of stirring speed, the microspheres were prepared at various speed such as at 1250, 1500, 1750 and 2000 rpm. Out of these, at 1500 rpm gave the formation of well formed microspheres with maximum encapsulation …show more content…
Vulcanization of rubber creates more disulfide bonds between chains, so that the chains tighten and makes rubber harder and less extensible.
The tensile strength of the various batches of natural rubber latex intravaginal ring containing drug in various concentration was estimated. The addition of drug in intravaginal ring used is inversely proportional to the tensile strength i.e., IVR containing 190 mg, 210mg drug and microsphere containing 190 mg of drug shows 0.438 KgF/cm2, 0.419 KgF/cm2, 0.309 KgF/cm2 and 0.218 KgF/cm2 of tensile strength respectively. This is due to addition of drug in IVR leads to reduction of natural rubber latex volume causing less disulfide bond formation.
B] Folding …show more content…
Hence 5 h time and 1000C temperature optimized for formation of further IVR batches. The folding endurance of the various batches of natural rubber latex intravaginal ring containing drug in various concentration was estimated. The addition of drug in intravaginal ring used is inversely proportional to the folding endurance i.e., IVR containing 190 mg, 210 mg drug and microsphere containing 190 mg of drug shows 336, 310, 241 and 174 folding endurance respectively. This is happened due to addition of drug in IVR leads to reduction of natural rubber latex volume in the ring causing less availability of rubber latex for disulfide bond formation.
6.3.2 In vitro drug release
The drug release from batch R2 i.e. IVR containing 210 mg drug, showed more release (42.17%) in 12 h as compared to other batches (Table 22). Subsequently, the slow release was obtained due to the leaching action. These rings showed a large initial burst of drug release in 12 h which slowly decreases over 24 h and to 120 h. The 12 h burst, representing release of the metronidazole initially present at the surface of the ring. During vulcanization insoluble film of rubber entrap the suspended drug particles, this his leads to decrease in penetration of dissolution fluid and leaching out of drug resulting in slow drug release. The drug release